Fe<Subscript>3</Subscript>O<Subscript>4</Subscript>@PAM@NTA-Ni<Superscript>2+</Superscript> Magnetic Composite Nanoparticles for Highly Specific Separation of His-tagged Proteins

A facile approach has been developed to synthesize Fe₃O₄@PAM (polyacrylamide) nanoparticles (NPs) with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe₃O₄ NPs aqueous suspension. Nitrilotriacetic acid (NTA) was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni²⁺ ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins (His-tagged GFP). The high magnetic property of Fe₃O₄@PAM@NTA-Ni²⁺ allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity (42 μg protein/mg of NPs). The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale.     

不同用量底肥对果蔗产量和品质的影响研究初报

试验结果表明:300 kg/hm2的底肥对果蔗出苗、株高、茎径、单株重、有效茎、多数商品性状和工艺性状具有正效应,对分蘖、蔗汁抽出率和抗螟性有轻微负效应,故其比不施底肥的增产24.52%,达极显著水平,是本试验中对果蔗效果最佳的底肥用量;600 kg/hm2的底肥对果蔗株高、茎径、单株重、部份商品性状、工艺性状和抗螟性有正效应,对出苗无影响,对分蘖、有效茎、多数商品性状有轻微负效应,故其比对照增产20.87%,达极显著水平,是本试验中对果蔗综合性状效果最好的底肥用量;900 kg/hm2的底肥对果蔗分蘖、株高、茎径、单株重、有效茎、少数商品性状、工艺性状和抗螟性具有正效应,对出苗、多数商品性状有负效应,故其增产显著,是本试验中对果蔗效果较好的底肥用量;1200 kg/hm2的底肥对果蔗分蘖、株高、茎径、单株重、田问锤度有轻微正效应,对其他性状则为负效应,是本试验中效果最差、唯一减产的底肥用量.     

First principles modeling of disorder scattering in graphene

It is important to investigate impurity scattering phenomena when modeling graphene nanoscale devices, as impurities are invariably present in any realistic system and can significantly influence graphene carrier transport. We present a short review of quantum transport where density functional theory (DFT) is carried out within the nonequilibrium Green’s function formalism (NEGF), focusing on a recent extension of this framework in the form of nonequilibrium vertex correction (NVC) that captures random graphene impurity scattering in a systematic fashion. Our results show that disorder effects significantly alters the electronic and transport properties of graphene devices. We argue that disorder effects should not be ignored if one were to model graphene nanoscale devices in realistic situations, including arriving at fundamental electronic properties such as Ohm’s law.     

高浓度超稠油乳化废水预处理工艺与实践

超稠油在加工过程中产生大量高浓度乳化废水,常规炼油污水处理场难以处理并导致外排水严重超标.以某稠油加工企业高浓度超稠油乳化废水为研究对象,对其物理化学性质,沉降、破乳、絮凝条件进行了系统研究,并在大量试验的基础上开发出预处理工艺.相应装置建成后运行平稳高效,废水得到高效的处理与污油资源回收.     

玻璃容器造型的计算机辅助设计

本文采用Bézier曲线和B样条曲线以及自动绘图技术,对玻璃容器造型进行计算机辅助设计,并对三种样条曲线作了分析和比较,给出了计算机程序框图,这将有利于模具的设计和制造。     

Modeling and finite element analysis of rod and wire steel rolling process

Two thermomechanical coupled elastic-plastic finite element （FE） models were developed for predicting the 12-pass continuous rolling process of GCrl 5 rod and wire steel. The distances between stands in the proposed models were set according to the actual values, and the billets were shortened in the models to reduce the calculation time. To keep the continuity of simulation, a technique was developed to transfer temperature data between the meshes of different models in terms of nodal parameters by interpolation functions. The different process variables related to the rolling process, such as temperature, total equivalent plastic strain, equivalent plastic strain rate, and contact friction force, were analyzed. Also, the proposed models were applied to analyze the reason for the occurrence of an excessive spread in width. Meanwhile, it was also utilized to assess the influence of the roll diameter change on the simulated results such as temperature and rolling force. The simulated results of temperature are found to agree well with the measured results.     

IR—UT钢水升温工艺实验

以苏州钢铁厂钢包为原型，通过水模和中频感应炉实验，确定了ＩＲ－ＵＴ－１６型精炼装置的设备参数和工艺参数，证实了采用Ａｌ或Ｓｉ－Ｃａ－Ｂａ－Ａｌ作发热剂进行钢水吹氧升温的可行性，钢水成分可满足小方坯连铸的要求。     

染料敏化太阳能电池对电极的研究进展

简要说明了染料敏化太阳能电池中对电极的作用,指出对电极是染料敏化太阳能电池的重要研究方向。详细介绍了各类对电极的特点和制备工艺,通过对比发现,铂对电极性能最好,但高成本限制了它的应用;价格低廉、性能较高的碳和聚合物对电极的发展前景广阔。最后论述了对电极的发展方向和国内外的研究现状。     

The h-p version of the finite element method

The paper is the second in the series of two analyzing the h-p version of the finite element method. It generalizes the results of the first part (Guo, Babuska 1986) and presents computational results.Supported by the NSF Grant DMS-8315216Partially supported by ONR Contract N00014-85-K-0169     

A π-extended tetrathiafulvene derivative: Synthesis and photoluminescence properties

Solid-state emissive organic materials have received increasing attention due to their prospective applications in large-area. Here, a novel π-extended tetrathiafulvene derivative DPD with donor-π-donor structure has been newly developed to produce the prominent characteristic of aggregation-induced enhanced emission (AIEE) that exhibits higher fluorescence quantum efficiency in solid film compare to its solutions. The AIEE mechanism of DPD was studied in detail through photophysical investigations and can be dominant attributed to the formed excimer state of DPD and J-aggregates in solid state.