Pathogen-group specific association between CXCR1 polymorphisms and subclinical mastitis in dairy heifers

The chemokine (C-X-C motif) receptor 1 (CXCR1) gene encodes the homonymous receptor for interleukin 8 (IL8) on polymorphonuclear neutrophilic leucocytes (PMNL). Binding causes migration from blood to milk, activation and prolonged survival of PMNL, a crucial process in the innate immune defence of the bovine mammary gland against invading mastitis-causing pathogens. The main objective of this study was to screen the entire coding region of the CXCR1 gene for polymorphisms and to analyse their association with udder health of dairy heifers. One-hundred-and-forty Belgian Holstein heifers originating from 20 commercial dairy farms were genotyped by DNA sequencing. Detailed phenotypic data on udder health was available including quarter bacteriological culture results and somatic cell count (SCC) in early lactation and composite milk SCC during first lactation. In total, 16 polymorphisms (including 8 missense mutations) were detected. Polymorphism c.980A>G was associated with pathogen-group specific IMI: heifers with genotype AG were less likely to have an IMI due to major mastitis pathogens compared with heifers with genotype GG but did not have less IMI by coagulase-negative staphylococci, so-called minor pathogens. CXCR1 genotype was neither associated with quarter SCC in early lactation nor with composite SCC during lactation. Although mastitis susceptibility is influenced by many factors, some genetic polymorphisms potentially have major effects on udder health of heifers, as was shown here. These results trigger us to further study the relationship between CXCR1 polymorphisms and mastitis susceptibility in both observational and experimental trials.     

Electronic Current Distribution Calculation for a Ni‐YSZ Solid Oxide Fuel Cell Anode

A simulation of a nickel‐yttrium stabilized zirconium oxide (Ni‐YSZ) solid oxide fuel cell cermet anode was used to determine the electronic current distribution within the percolating networks of nickel particles distributed in the electrode. The anode is simulated via a Monte–Carlo percolation model and current distribution is calculated via a relaxation algorithm. Nickel particle current densities are reported as a ratio to the total anode current density allowing results to be applied to any anode current density. Calculated current distributions were drastically affected by the volume percent of nickel as well as anode porosity. Experiments were performed to determine failure current densities of thin nickel wires to establish the relationship between critical current densities and surface area or volume of the wires. Both reducing and oxidizing environments were used for these measurements over a temperature range up to 800 °C.     

Long-term personal exposure to traffic-related air pollution among school children, a validation study

Several recent studies suggest an association between long-term exposure to traffic-related air pollution and health. Most studies use indicators of exposure such as outdoor air pollution or traffic density on the street of residence. Little information is available about the validity of these measurements as an estimate of long-term personal exposure to traffic-related air pollution. In this pilot study, we assessed outdoor and personal exposure to traffic-related air pollution in children living in homes on streets with different degree of traffic intensity. The personal exposure of 14 children aged 9-12 years to 'soot', NO(x) (NO and NO(2)) was assessed in Amsterdam between March and June 2003. Each child's personal exposure was monitored during four repeated 48-h periods. Concurrently, in- and outdoor NO(x) measurements were carried out at the school and at the home of each participating child. Measurements were supplemented by a questionnaire on time activity patterns and possible indoor sources. Flow-controlled battery operated pumps in a made-to-fit backpack were used to sample personal exposure to 'soot', determined from the reflectance of PM(2.5) filters. Exposure to NO(x) was assessed using Ogawa passive samplers. Children living near busy roads were found to have a 35% higher personal exposure to 'soot' than children living at an urban background location, despite that all children attended the same school that was located away from busy roads. Smaller contrasts in personal exposure were found for NO (14%), NO(2) (15%) and NO(x) (14%). This finding supports the use of 'living near a busy road' as a measure of exposure in epidemiological studies on the effects of traffic-related air pollution in children.     

Social Exclusion in Later Life: A Systematic Review of the Literature

In the last decade, there has been a growing awareness regarding social exclusion. Considering the ageing population and the likelihood of older people being socially excluded, the aims of this article are to: (1) review existing studies concerning social exclusion in later life; and (2) identify how environmental and life-course perspectives are presented in studies focusing on social exclusion in later life. A systematic review in seven scientific databases was conducted to explore the peer-reviewed evidence. In total, 26 articles were included and analysed. Findings describe the variety of methods, conceptualisation, dimensions and measures used in this recent area of research. Determinants of social exclusion in later life are discussed and life-course and environmental perspectives are examined. The discussion highlights the complex character of the concept and measurement of social exclusion, and the presence of general and age-specific dimensions of social exclusion in later life. The time and context relativity and the need for life-course and environmental perspectives on social exclusion in later life are discussed. Finally, future directions of research are discussed.     

Effect of Product Microstructure and Process Parameters on Modified Atmosphere Packaged Bread

The objective of this study was to examine bread microstructure, oxygen diffusion properties, and gas exchange between bread and headspace after packaging in modified atmosphere (MA). Breads were produced at laboratory scale, and industrially produced breads were included as a reference. X-ray microtomography was applied to characterize the microstructure of the bread samples. For each sample type, oxygen diffusivity was calculated based on microstructural parameters. The samples for gas analyses were packaged under MA using vacuum compensation, using two vacuum strengths during the air evacuation step. The total porosity of all laboratory samples was above 75%, except for the side and bottom crusts which had a lower porosity (<70%). The porosities of the crumb and the side crust of the commercial bread were 80 and 76%, respectively. The connectivity density of the crumbs of the laboratory and commercial breads was 8 and 10 mm^?3, respectively. The crust showed a larger resistance to oxygen diffusion than the crumb, but for both bread regions, the diffusivity was maximally ten times smaller than that of oxygen in air. When considering all data obtained from the gas analysis in the headspace immediately after packaging, oxygen levels of 0.1 ± 0.1% for strong vacuum and 3.8 ± 2.9% for weak vacuum were obtained, which differed significantly (p < 0.05). The results of the laboratory samples corresponded well to those of industrially baked breads. It was concluded that vacuum strength during packaging determines gas transport in bread after packaging.     

Neutralized electrolyzed water efficiently reduces Fusarium spp. in vitro and on wheat kernels but can trigger deoxynivalenol (DON) biosynthesis

Fusarium Head Blight (FHB), caused by a blend of Fusarium species, is a destructive fungal disease of wheat and other small grain cereals. FHB has become an important issue in food and feed industry. Moreover, the majority of FHB pathogens have the ability to synthesize a range of mycotoxins. Although several physical and chemical control measures can be taken to control these fungi in the field, research is needed to provide new techniques for control during storage and transport of cereals. Mounting evidence shows that electrolyzed oxidizing water (EOW) has antimicrobial activity and might be a useful alternative for conventional control measures. The objective of the present work, was to investigate the influence of EOW on outgrowth and germination of Fusarium spp. and deoxynivalenol (DON) production. Both an in vitro and in vivo approach were pursued. In a first approach, a screening of the main FHB causing species was conducted. Secondly, the effect of EOW on Fusarium graminearum and the effect on DON biosynthesis was investigated using a trichothecene knockout mutant. These experiments showed an increase in DON levels upon sub lethal amendments of EOW to F. graminearum spores. In addition, the reactive oxygen species H₂O₂ was shown to govern this induction.Finally, the work was validated on a laboratory scale via an in vivo assay using wheat grains in which the Fusarium outgrowth was measured. The present work demonstrates that EOW has potential to control Fusarium spp. in wheat grains during transport and storage although sub lethal concentrations can result in increased DON biosynthesis.     

Silhouette-based multi-sensor smoke detection

Fire is one of the leading hazards affecting everyday life around the world. The sooner the fire is detected, the better the chances are for survival. Today’s fire alarm systems, such as video-based smoke detectors, however, still pose many problems. In order to accomplish more accurate video-based smoke detection and to reduce false alarms, this paper proposes a multi-sensor smoke detector which takes advantage of the different kinds of information represented by visual and thermal imaging sensors. The detector analyzes the silhouette coverage of moving objects in visual and long-wave infrared registered (~aligned) images. The registration is performed using a contour mapping algorithm which detects the rotation, scale and translation between moving objects in the multi-spectral images. The geometric parameters found at this stage are then further used to coarsely map the silhouette images and coverage between them is calculated. Since smoke is invisible in long-wave infrared its silhouette will, contrarily to ordinary moving objects, only be detected in visual images. As such, the coverage of thermal and visual silhouettes will start to decrease in case of smoke. Due to the dynamic character of the smoke, the visual silhouette will also show a high degree of disorder. By focusing on both silhouette behaviors, the system is able to accurately detect the smoke. Experiments on smoke and non-smoke multi-sensor sequences indicate that the automated smoke detection algorithm is able to coarsely map the multi-sensor images. Furthermore, using the low-cost silhouette analysis, a fast warning, with a low number of false alarms, can be given.     

The flavonoid tangeretin activates the unfolded protein response and synergizes with imatinib in the erythroleukemia cell line K562

We explored the mechanism of cell death of the polymethoxyflavone tangeretin (TAN) in K562 breakpoint cluster region‐abelson murine leukemia (Bcr‐Abl+) cells. Flow cytometric analysis showed that TAN arrested the cells in the G₂/M phase and stimulated an accumulation of the cells in the sub‐G₀ phase. TAN‐induced cell death was evidenced by poly(ADP)‐ribose polymerase cleavage, DNA laddering fragmentation, activation of the caspase cascade and downregulation of the antiapoptotic proteins Mcl‐1 and Bcl‐x_L. Pretreatment with the pancaspase inhibitor Z‐VAD‐FMK_blocked caspase activation and cell cycle arrest but did not inhibit apoptosis which suggest that other cell killing mechanisms like endoplasmic reticulum (ER)‐associated cell death pathways could be involved. We demonstrated that TAN‐induced apoptosis was preceded by a rapid activation of the proapoptotic arm of the unfolded protein response, namely PKR‐like ER kinase. This was accompanied by enhanced levels of glucose‐regulated protein of 78 kDa and of spliced X‐box binding protein 1. Furthermore, TAN sensitized K562 cells to the cell killing effects of imatinib via an apoptotic mechanism. In conclusion, our results suggest that TAN is able to induce apoptosis in Bcr‐Abl+ cells via cell cycle arrest and the induction of the unfolded protein response, and has synergistic cytotoxicity with imatinib.     

Electrical and flexural anisotropy in freeze tape cast stainless steel porous substrates

Engineered metal foams have strong potential in applications such as fuel cell electrodes, sensors, variable springs, filtering media, and compositionally graded composite structures. In this study, the variation in mechanical and electrical properties was characterized in engineered porous metal foams with aligned porosity to ascertain the degree of anisotropy that can be induced in these substrates. Porous substrates were prepared by freeze tape casting of powdered ferritic stainless steel. After directional solidification of the slurry, the solvent was sublimed from ~ 1 mm thick tapes, yielding porous green metal compacts which were sintered in a protective atmosphere. The resulting disks exhibit long range ordered acicular pores with substantial anisotropy in the mechanical and electrical properties that is related to the cross sectional pore morphology and connectivity. DC conductivity testing revealed up to 61% variation depending on direction of measurement relative to the alignment of pores. Also, an 89% variation in flexural rigidity in relation to pore orientation was observed in identical disks.