L'attachment à l'age scolaire et le développement des troubles de comportement.

The purpose of this longitudinal study was to examine the association between the mother–child attachment relationship and behavioural problems among a test group of 120 Francophone Canadian children from mixed social and economic backgrounds. The Strange Situation protocol was used to measure attachment styles when the children were between the ages of 5 and 7 yrs. Each child's behavioural difficulties were assessed by his or her educator or teacher during 3 developmental periods: 3–5 yrs, 5–7 yrs, and 7–9 yrs. Results show that disorganized insecure attachment is associated with an increased risk of developing exteriorized and interiorized behavioural troubles between the ages of 5 and 7. The authors also observed exteriorized behavioural problems among children with anxious-ambivalent attachment, especially in the 3–5 age group. Avoidant children, usually assessed positively, received exteriorization scores during the 5–7 period which were significantly below those of securely attached children. Avoidant boys also exhibited more interiorized symptoms during this time-frame. Analyses of the persistence of behavioural problems also showed increased risk among disorganized and ambivalent children with secure or avoidant attachment styles. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Glass-Ceramic Formation in the ZnO-P2O5 System and the Effect of Silica as a Nucleating Agent

Crystallization of a series of ZnO-P₂O₅ based glasses was investigated. ZnO-P₂O₅-CaO glasses could be converted most readily to glass-ceramics and crystallization of these led to formation of alpha-Zn₂P₂O₇, alpha-CaZn₂(PO₄)₂, and ß-CaZn₂(PO₄)₂ phases. A further phase has been tentatively identified as monoclinic (Zn,Ca)₂P₂O₇. The most promising glass-ceramic composition Z15 (59.4ZnO·33P₂O₅·6.6CaO·1SiO₂) crystallized to alpha-Zn₂P₂O₇ and ß-CaZn₂(PO₄)₂, the latter phase being stabilized by the presence of SiO₂ which also encouraged volume nucleation giving a fine-scale (submicrometer) microstructure.     

Characterizing SERCA Function in Murine Skeletal Muscles after 35–37 Days of Spaceflight

It is well established that microgravity exposure causes significant muscle weakness and atrophy via muscle unloading. On Earth, muscle unloading leads to a disproportionate loss in muscle force and size with the loss in muscle force occurring at a faster rate. Although the exact mechanisms are unknown, a role for Ca²⁺ dysregulation has been suggested. The sarco(endo)plasmic reticulum Ca²⁺ ATPase (SERCA) pump actively brings cytosolic Ca²⁺ into the SR, eliciting muscle relaxation and maintaining low intracellular Ca²⁺ ([Ca²⁺]_i). SERCA dysfunction contributes to elevations in [Ca²⁺]_i, leading to cellular damage, and may contribute to the muscle weakness and atrophy observed with spaceflight. Here, we investigated SERCA function, SERCA regulatory protein content, and reactive oxygen/nitrogen species (RONS) protein adduction in murine skeletal muscle after 35–37 days of spaceflight. In male and female soleus muscles, spaceflight led to drastic impairments in Ca²⁺ uptake despite significant increases in SERCA1a protein content. We attribute this impairment to an increase in RONS production and elevated total protein tyrosine (T) nitration and cysteine (S) nitrosylation. Contrarily, in the tibialis anterior (TA), we observed an enhancement in Ca²⁺ uptake, which we attribute to a shift towards a faster muscle fiber type (i.e., increased myosin heavy chain IIb and SERCA1a) without elevated total protein T-nitration and S-nitrosylation. Thus, spaceflight affects SERCA function differently between the soleus and TA.     

First confirmation in red wine of products resulting from direct anthocyanin–tannin reactions

Analysis of wine fractions before and after thiolysis confirmed the occurrence in red wine of direct reactions between anthocyanins and tannins established earlier in model solutions. Results showed the presence of two types of structures differing in the linkage position of the anthocyanin moiety. On one hand, detection of malvidin‐3‐glucoside (Mv3g) among thiolysis products revealed the presence of anthocyanin‐derived pigments in which Mv3g is linked by its C‐6 or C‐8 top. On the other hand, LC/MS analysis allowed the detection of two derivatives tentatively identified as flavenes or a bicyclic condensation products yielded by the reaction of a flavanol monomer (C‐6 or C‐8 top) with malvidin‐3‐glucoside (C‐4 position). The presence of the corresponding benzylthioethers after thiolysis of the polymeric fractions confirmed that procyanidins are similarly involved in the latter reaction. Besides, MS also allowed the detection of new benzylthioethers of catechin derivatives released after thiolysis of the wine fractions, indicating that in addition to the postulated processes other types of reactions take place in wines. © 2000 Society of Chemical Industry     

Regulation of Epigenetic Modifications in the Placenta during Preeclampsia: PPARγ Influences H3K4me3 and H3K9ac in Extravillous Trophoblast Cells

The aim of this study was to analyze the expression of peroxisome proliferator-activated receptor γ (PPARγ) and retinoid X receptor α (RxRα), a binding heterodimer playing a pivotal role in the successful trophoblast invasion, in the placental tissue of preeclamptic patients. Furthermore, we aimed to characterize a possible interaction between PPARγ and H3K4me3 (trimethylated lysine 4 of the histone H3), respectively H3K9ac (acetylated lysine 9 of the histone H3), to illuminate the role of histone modifications in a defective trophoblast invasion in preeclampsia (PE). Therefore, the expression of PPARγ and RxRα was analyzed in 26 PE and 25 control placentas by immunohistochemical peroxidase staining, as well as the co-expression with H3K4me3 and H3K9ac by double immunofluorescence staining. Further, the effect of a specific PPARγ-agonist (Ciglitazone) and PPARγ-antagonist (T0070907) on the histone modifications H3K9ac and H3K4me3 was analyzed in vitro. In PE placentas, we found a reduced expression of PPARγ and RxRα and a reduced co-expression with H3K4me3 and H3K9ac in the extravillous trophoblast (EVT). Furthermore, with the PPARγ-antagonist treated human villous trophoblast (HVT) cells and primary isolated EVT cells showed higher levels of the histone modification proteins whereas treatment with the PPARγ-agonist reduced respective histone modifications. Our results show that the stimulation of PPARγ-activity leads to a reduction of H3K4me3 and H3K9ac in trophoblast cells, but paradoxically decreases the nuclear PPARγ expression. As the importance of PPARγ, being involved in a successful trophoblast invasion has already been investigated, our results reveal a pathophysiologic connection between PPARγ and the epigenetic modulation via H3K4me3 and H3K9ac in PE.     

Steady viscous flow in constricted elastic tubes subjected to a uniform external pressure

Cardiovascular illness is most commonly caused by a constriction, called a stenosis. A non-linear mathematical model with a free moving boundary was introduced to study viscous flow in tapered elastic tubes with axisymmetric constrictions subject to a prescribed pressure drop and a uniform external pressure. An iterative numerical scheme using a boundary iteration method was developed to solve the model. Effects of stenosis severity and stiffness, pressure drop, external pressure and stiffness of the vessel wall on the flow and wall motion were evaluated. It was found that stenosis severity, pressure drop and external pressure played more dominant roles than tube wall stiffness and stenosis stiffness perturbation. Tubes with 71 and 78 per cent stenoses showed two areas of negative transmural pressure and complex contraction–expansion–contraction wall motion patterns. Two types of tube diameter contraction and negative transmural pressure were observed, one was just distal to the stenosis and the other was near the outlet of the tube. Experiments using stenotic silicone tubes were conducted to quantify the tube law and verify the predicted pressure–flow relationship. The agreement between the numerical results and experimental measurements is better than that from a previous model which assumed periodicity of the tube and imposed different pressure conditions. © 1998 John Wiley & Sons, Ltd.     

Growth Kinetics and Characterization of Chromia Scales Formed on Ni–30Cr Alloy in Impure Argon at 700 °C

Oxidation of Metals - The oxidation of a Ni–30Cr alloy at 700 °C in impure argon was studied in order to provide new elements of understanding on chromia scale growth in low...     

Adaptive Resource Management Platform for Reconfigurable Networks

Users’ expectations towards technology, in terms of quality, service availability and accessibility are ever increasing. Aligned with this, the wireless world is rapidly moving towards the next generation of systems, featuring cooperating and reconfiguring capabilities for coexisting (and upcoming) Radio Access Technologies (RATs), so that to improve connectivity and reduce costs. In this respect, conventional planning and management techniques ought to be replaced by advanced schemes that consider multidimensional characteristics, increased complexity and high speeds. To this effect, means are needed capable to support scalability and to cater for advanced service features, provided to users at high rates and cost-effectively. This article provides a scheme to optimize resource management in future systems, by describing a platform that accommodates engineering mechanisms that deal with dynamic, demand driven planning and managing of spectrum and radio resources in reconfigurable networks. To do so, it first discusses the fundamentals and the approach followed in the proposed architecture and then investigates the basic functional modules. The architecture is validated through a set of use-cases that exemplify the operational applicability and efficiency in a wide range of communication scenarios.

Remarkable findings concerning PBDEs in the terrestrial top-predator red fox (Vulpes vulpes)

In the present study, we have analyzed muscle, liver, and adipose tissue of 33 red foxes from Belgium for their content of polybrominated diphenyl ethers (PBDEs). Median sums of seven tri- to hepta-BDEs (BDE 28, BDE 47, BDE 99, BDE 100, BDE 153, BDE 154, and BDE 183) were 2.2, 2.4, and 3.4 ng/g lipid weight in adipose tissue, liver, and muscle, respectively. These levels were lower than those found in various species of voles and mice, the main prey species of the red fox. This is probably related to the high capacity of the foxes to metabolize and eliminate lower brominated congeners. BDE 209 generally dominated the PBDE congener profiles in the red fox samples. In samples containing BDE 209, this congener contributed, on the average, approximately 70% to the total PBDE content. BDE 209 was measured in concentrations as high as 760 ng/g lipid weight in the liver, but the detection frequency was not more than 40%. In animals with the highest BDE 209 levels, this congener was detected in muscle, liver, as well as in adipose tissue. Other abundant congeners were BDE 153 and BDE 47, which prevail in other terrestrial species. The particular PBDE congener profile observed in the red fox resembles that seen in grizzly bears from Canada, but differs from those previously reported for terrestrial avian species. Our data confirms unambiguously that BDE 209 does bioaccumulate in terrestrial top predators, such as the red fox.     

Rapid identification of long-chain polyunsaturated fatty acids in a marine extract by HPLC-MS using data-dependent acquisition

The collision-induced dissociation (CID) of a range of deprotonated fatty acid standards was studied using linear ion trap mass spectrometry. Neutral losses of 78, 98, and 136 Da were consistently observed for fatty acids with five or more double bonds. Comparison of the MS/MS spectra of docosahexaenoic acid (DHA) and universally (13)C-labeled DHA allowed the molecular formulas for these neutral losses to be determined as C(6)H(6), C(5)H(6)O(2), and C(8)H(8)O(2). Knowledge of fatty acid fragmentation processes was then applied to identify fatty acids from a sea anemone, Aiptasia pulchella, and dinoflagellate symbiont, Symbiodinium sp. extract. Using HPLC-MS, fatty acids were separated and analyzed by tandem mass spectrometry in data-dependent acquisition mode. Neutral loss chromatograms for 78, 98, and 136 Da allowed the identification of long-chain fatty acids with five or more double bonds. On the basis of precursor ion m/z ratios, chain length and degree of unsaturation for these fatty acids were determined. The application of this technique to an Aiptasia sp.-Symbiodinium sp. lipid extract enabled the identification of the unusual, long-chain fatty acids 24:6, 26:6, 26:7, 28:7, and 28:8 during a single 40 min HPLC-MS analysis.