Colorimetrische Bestimmung von Nitrat und Nitrit in biologischem Material

Zusammenfassung Die ausgearbeitete Methode zur Nitrat- und Nitritbestimmung beruht auf der colorimetrischen Bestimmung des durch Nitrierung entstehenden 6-Nitro-2,4-xylenols.Die Nitrite und Nitrate werden nebeneinander durch zwei Messungen bestimmt. Bei der ersten Messung werden Nitrite zu Nitraten oxydiert, bei der zweiten werden sie nach Überführung zu Methylnitrit entfernt. Die Differenz der ermittelten Werte entspricht den anwesenden Nitriten.Diese Methode eignet sich für Nitrat- und Nitritbestimmung im biologischen Material.     

Recent Progress in Neural Probes Using Silicon MEMS Technology

Extracellular recordings from the brain are the basis for the fundamental understanding of the complex interaction of electrical signals in neural information transfer. Going beyond wire electrodes and bundles of electrode wires such as tetrodes, multielectrode arrays based on silicon technologies are receiving growing attention, since they enable a pronounced increase in the number of recording sites per probe shaft. In this paper, recent innovations contributed by the authors to the development of probe arrays based on microelectromechanical system (MEMS) technologies within the EU‐funded research project NeuroProbes are described. The resulting structures include passive electrode arrays based on single‐shaft and four‐shaft probes comprising nine planar electrodes per shaft with lengths of up to 8 mm. Further, active probe arrays with complementary metal–oxide–semiconductor (CMOS) circuitry integrated on the probe shaft, enabling the arrangement of 188 electrodes in two columns along a 4‐mm‐long probe shaft with an electrode pitch of only 40 µm, are described. These active probes were developed for an electronic depth control. Further, the paper reports assembly technologies for combining the probe arrays with highly flexible ribbon cables. Applications of the probes in in vivo experiments are summarized. © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

Biodiesel influence on tribology characteristics of a diesel engine

This paper deals with the influence of biodiesel on some tribology characteristics of a bus diesel engine with a mechanically controlled fuel injection system. The tests have been performed on a fully equipped engine test bed, on a fuel injection test bed and on a discharge coefficient testing device. The tested fuel was neat biodiesel produced from rapeseed. Attention was focused on the biodiesel influence on the pump plunger surface roughness, on the carbon deposits in the combustion chamber, on the injector and in the injector nozzle hole. The pump plunger surface was analyzed by experimentally determined roughness parameters and by a microscope. The carbon deposits at fuel injector and in the combustion chamber were examined using endoscopic inspection. The deposits in the injector nozzle were investigated indirectly by measuring the nozzle discharge coefficient. Numerical simulation has been performed in order to estimate the influence of the discharge coefficient variation on the computed injection characteristics. The obtained results indicate that biodiesel usage may even improve the pump plunger lubrication conditions. Furthermore, the carbon deposits in the combustion chambers did not vary significantly in quantity but they were noticeably redistributed. Finally, it was found out that the variation of the nozzle discharge coefficient has to be taken into account only if high accuracy of numerical simulation is desired.     

Risk Budget management in progressing underground works: International Society for Trenchless Technology (ISTT) and International Tunnelling Association (ITA) Joint Working Group Report

There is a mass of detailed data concerning technical risk assessment methods and practices for underground work. But there is very little advice or guidance on the broad apportionment of the total risk between the various phases of an underground project or general advice on how risk might be managed. The Working Group has produced a generic Risk Budget covering five typical phases of an underground works project, which illustrates the heavy bias of risk towards the early phases. Using a practical example the report illustrates how project risk can be managed in a structured manner.     

Computer-aided inspection center for magnetoimpedance spectroscopy

Principles of measuring magnetoimpedance effect in samples that are intended for constructing electronic circuits that contain sensing elements for detecting weak magnetic fields, including stray fields, are described. Special attention is paid to those existent techniques of measuring magnetoimpedance effect in magnetic structures with distinctly manifested magnetic anisotropy that have already been used in or look promising for systems of magnetic nondestructive testing. The merits and drawbacks of a computer-aided magnetoimpedance-spectroscopy facility that has been designed at Ural Federal University are discussed in detail. The facility is based on an Agilent E4991a impedance analyzer that has been adapted for studying a wide class of magnetic structures.     

3-D Optical Interference Microscopy at the Lateral Resolution

For applications in micro- and nanotechnologies the lateral resolution of optical 3-D microscopes becomes an issue of increasing relevance. However, lateral resolution of 3-D microscopes is hard to define in a satisfying way. Therefore, we first study the measurement capabilities of a highly resolving white-light interference (WLI) microscope close to the limit of lateral resolution. Results of measurements and simulations demonstrate that better lateral resolution seems to be achievable based on the envelope evaluation of a WLI signal. Unfortunately, close to the lateral resolution limit errors in the measured amplitude of micro-structures appear. On the other hand, results of interferometric phase evaluation seem to be strongly low-pass filtered in this case.

Furthermore, the instrument transfer characteristics and the lateral resolution capabilities of WLI instruments are also affected by polarization. TM polarized light is less sensitive to edge diffraction and thus systematic errors can be avoided. However, apart from ghost steps due to fringe order errors, the results of phase evaluation seem to be closer to the real surface topography if TE polarized light is used. The lateral resolution can be further improved by combining WLI and structured illumination microscopy. Since the measured height of rectangular profiles close to the lateral resolution limit is generally too small compared to the real height, we introduce a method based on phase evaluation which characterizes the heights of barely laterally resolved rectangular gratings correctly.     

Application of entropy formulas in detection of denial‐of‐service attacks

The paper compares five entropy formulas (Shannon, Tsallis, Rényi, Bhatia‐Singh, and Ubriaco) and their application in the detection of distributed denial‐of‐service (DDoS) attacks. The Shannon formula has been used extensively for this purpose for more than a decade. The use of the Tsallis and Rényi formulas in this context has also been proposed. Bhatia‐Singh entropy is a novel information metric with promising results in initial applications in this area. Ubriaco proposed an entropy function based on the fractional calculus. In this paper, flow size distribution was used as the input for detection. The type of DDoS attack is SYN flood, and simulation was used to obtain the input dataset. The results show that the Rényi and Bhatia‐Singh detectors perform better than the rest. Rényi and Tsallis performed similarly with respect to the true positive rate, but Rényi had a much lower false positive rate. The Bhatia‐Singh detector had the best true positive rate but a higher false positive rate than Rényi. The Ubriaco detector performed similar to the Shannon detector. With respect to detection delay, Tsallis, Ubriaco, and Shannon produced similar results, with a slight advantage associated with the Ubriaco detector, while Rényi and Bhatia‐Singh had a larger detection delay than the former three.     

Bioinspired 3D Printed Locomotion Devices Based on Anisotropic Friction

Anisotropic friction plays a key role in natural systems, particularly for realizing the purpose of locomotion and strong attachment for the survival of organisms. Of particular interest, here, is the observation that friction anisotropy is promoted numerous times by nature, for example, by wild wheat awn for its targeted and successful seed anchorage and dispersal. Such feature is, however, not fully exploited in man‐made systems, such as microbots, due to technical limitations and lack of full understanding of the mechanisms. To unravel the complex dynamics occurring in the sliding interaction between anisotropic microstructured surfaces, the friction induced by asymmetric plant microstructures is first systematically investigated. Inspired by this, anisotropic polymer microactuators with three‐dimensional (3D) printed microrelieves are then prepared. By varying geometric parameters, the capability of microactuators to generate strong friction anisotropy and controllable motion in remotely stretched cylindrical tubes is investigated. Advanced theoretical models are proposed to understand and predict the dynamic behavior of these synthetic systems and to shed light on the parameters and mechanisms governing their behavior. Finally, a microbot prototype is developed and cargo transportation functions are successfully realized. This research provides both in‐depth understanding of anisotropic friction in nature and new avenues for developing intelligent actuators and microbots.