Analytical correlation and interpretation of full scale containment specimen tests

The work presented in this paper is part of an EPRI-sponsored research program to develop experimentally verified methodology for predicting failure modes and leakage characteristics of concrete containments. This paper deals specifically with recent results of the analytical correlation and interpretation of full scale containment specimen tests. The tests under consideration are a wall/skirt-basemat specimen of a typical prestressed concrete containment, a specimen with a flawed liner to study liner crack growth, and a specimen with a typical steampipe penetration. Computational models of specimens are described, and pre-test and post-test analysis results are presented. The importance of local effects is discussed, and the role of specimen tests and analysis in failure prediction of containment structures is summarized.     

Estimating WCET using prediction models to compute fitness function of a genetic algorithm

Real-Time Systems - Genetic algorithms can be used to generate input data in a real-time system that produce the worst-case execution time of a task. While generating the test data, the fitness...     

Interventions for long-term software security: Creating a lightweight program of assurance techniques for developers

Though some software development teams are highly effective at delivering security, others either do not care or do not have access to security experts to teach them how. Unfortunately, these latter teams are still responsible for the security of the systems they build: systems that are ever more important to ever more people. We propose that a series of lightweight interventions, six hours of facilitated workshops delivered over three months, can improve a team's motivation to consider security and awareness of assurance techniques, changing its security culture even when no security experts are involved. The interventions were developed after an Appreciative Inquiry and Grounded Theory survey of security professionals to find out what approaches work best. We tested the interventions in a participatory action research field study where we delivered the workshops to three software development organizations and evaluated their effectiveness through interviews beforehand, immediately afterwards, and after twelve months. We found that the interventions can be effective with teams with limited or no security experience and that improvement is long-lasting. This approach and the learning points arising from the work here have the potential to be applied in many development teams, improving the security of software worldwide.     

Experimental results on the coolability of a debris bed with multidimensional cooling effects

Within the reactor safety research, the removal of decay heat from a debris bed (formed from corium and residual water) is of great importance. In order to investigate experimentally the long term coolability of debris beds, the scaled test facility “DEBRIS” (Fig. 1) has been built at IKE. A large number of experiments had been carried out to investigate the coolability limits for different bed configurations ( [Rashid et al., 2008], [Groll et al., 2008] and [0055]). Analyses based on one-dimensional configurations underestimate the coolability in realistic multidimensional configurations, where lateral water access and water inflow via bottom regions are favoured. Following the experiments with top- and bottom-flooding flow conditions this paper presents experimental results of boiling and dryout tests at different system pressures based on top- and bottom-flooding via a down comer configuration.A down comer with an internal diameter of 10 mm has been installed at the centre of the debris bed. The debris bed is built up in a cylindrical crucible with an inner diameter of 125 mm. The bed of height 640 mm is composed of polydispersed particles with particle diameters 2, 3 and 6 mm. Since the long term coolability of such particle bed is limited by the availability of coolant inside the bed and not by heat transfer limitations from the particles to the coolant, the bottom inflow of water improves the coolability of the debris bed and an increase of the dryout heat flux can be observed. With increasing system pressure, the coolability limits are enhanced (increased dryout heat flux).     

Purification and characterization of a novel glucoamylase from Fusarium solani

Thermostable enzymes are currently being investigated to improve industrial processes of starch saccharification. A novel glucoamylase was purified to electrophoretic homogeneity from the culture supernatant of Fusarium solani on a fast protein liquid chromatographic system (FPLC). The recovery of glucoamylase after gel filtration on FPLC was 31.8% with 26.2-fold increase in specific activity. The enzyme had a molecular mass of 40 kDa by SDS-PAGE and 41 kDa by gel filtration. The glucoamylase exhibited optimum activity at pH 4.5. The K_cat and K_m were 441/min and 1.9 mg/ml, respectively, for soluble starch, specificity constant (K_cat/K_m) was 232. The enzyme was thermally stable at 50 °C and retained 79% activity after 60 min at this temperature. The half-life of the enzyme was 26 min at 60°C. The enzyme was slightly stimulated by Cu²⁺ and Mg²⁺ and strongly inhibited by Hg²⁺, Pb²⁺, Zn²⁺, Ni²⁺ and Fe³⁺.     

FUS Microphase Separation: Regulation by Nucleic Acid Polymers and DNA Repair Proteins

Fused in sarcoma (FUS) is involved in the regulation of RNA and DNA metabolism. FUS participates in the formation of biomolecular condensates driven by phase transition. FUS is prone to self-aggregation and tends to undergo phase transition both with or without nucleic acid polymers. Using dynamic light scattering and fluorescence microscopy, we examined the formation of FUS high-order structures or FUS-rich microphases induced by the presence of RNA, poly(ADP-ribose), ssDNA, or dsDNA and evaluated effects of some nucleic-acid-binding proteins on the phase behavior of FUS–nucleic acid systems. Formation and stability of FUS-rich microphases only partially correlated with FUS’s affinity for a nucleic acid polymer. Some proteins—which directly interact with PAR, RNA, ssDNA, and dsDNA and are possible components of FUS-enriched cellular condensates—disrupted the nucleic-acid-induced assembly of FUS-rich microphases. We found that XRCC1, a DNA repair factor, underwent a microphase separation and formed own microdroplets and coassemblies with FUS in the presence of poly(ADP-ribose). These results probably indicated an important role of nucleic-acid-binding proteins in the regulation of FUS-dependent formation of condensates and imply the possibility of the formation of XRCC1-dependent phase-separated condensates in the cell.     

The relation between ketone bodies, glucose and minerals contents in bovine blood under various physiological conditions

The interrelationship between the concentration of ketone bodies, glucose, minerals (Na, K, Ca, Mg, P, Cl) in serum and in TCA soluble whole blood under different physiological conditions in 110 cows was investigated. There was a certain relationship (partly high significant) between the mineral content, ketone bodies and glucose levels in blood. However, there is no clear explanation for the qualification of blood minerals as an indicator of the content of ketone bodies and glucose in serum and whole blood. The age of animals and the seasons had no influence on the concentration of ketone bodies in serum and whole blood. Significant positive relationship was found between the time of lactation, pregnancy and serum glucose and negative relation with ketone bodies. There was no correlation between milk production and ketone bodies. The hours after feeding were positively correlated with ketone bodies.