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991.
The convergent correlator is widely used but it presents the drawback of the alignment requirements: fine focusing of the input scene Fourier transform on the filter plane, filter centering, scaling the scene Fourier transform to match the filter size, and azimuth matching of the filter with the input scene. We propose a set of tests to obtain a precise alignment of the convergent correlator. These methods are based on frequency filtering properties and they are applicable either for amplitude input or for phase-encoded input. The tests we present allow us to fulfill all the alignment requirements. The theory on which these tests are based is explained. The experimental results obtained during the alignment procedure are presented. We show some additional verifications of the correct alignment of the convergent correlator. 相似文献
992.
We present a manufacturing method based on the dynamic use of phase plates to photowrite Bragg gratings. This process allows for control of the local value of the index modulation envelope in the grating. The application to apodized fiber Bragg gratings is discussed. 相似文献
993.
Sensitive photoacoustic detection of ammonia with near-infrared diode lasers (1.53 microns) and a novel differential acoustic resonator is described; a sensitivity of 0.2 parts per million volume (signal-to-noise ratio = 1) is attained. To eliminate adsorption-desorption processes of the polar NH3 molecules, a relatively high gas flow of 300 SCCM was used for the ammonia-nitrogen mixture. The results are compared with recent ammonia measurements with a NIR diode and absorption spectroscopy used for detection and photoacoustic experiments performed with an infrared quantum-cascade laser. The performance of the much simpler and more compact setup introduced here was comparable with these previous state-of-the-art measurements. 相似文献
994.
Cervantes-Martínez J Flores-Hernández R Rodríguez-Garay B Santacruz-Ruvalcaba F 《Applied optics》2002,41(13):2541-2545
Greenhouse-grown plants of Agave tequilana Weber var. azul were inoculated with Erwinia carotovora, the causal agent of stem soft rot. We investigated the laser-induced fluorescence (LIF) of agave plants to determine whether LIF can be used as a noninvasive sensing tool for pathological studies. The LIF technique was also investigated as a means of detecting the effect of the polyamine biosynthesis inhibitor beta-hydroxyethylhydrazine as a bactericide against the pathogenic bacterium Erwinia carotovora. A He-Ne laser at 632.8 nm was used as the excitation source, and in vivo fluorescence emission spectra were recorded in the 660-790-range. Fluorescence maxima were at 690 and 740 nm. The infected plants that were untreated with the bactericide showed a definite increase in fluorescence intensity at both maxima within the first three days after infection. Beginning on the fifth day, a steady decrease in fluorescence intensity was observed, with a greater effect at 740 than at 690 nm. After 30 days there was no fluorescence. The infected plants that had been treated with the bactericide showed no significant change in fluorescence compared with that of the uninfected plants. The ratio of fluorescence intensities was determined to be F 690 nm/F 740 nm for all treatments. These studies indicate that LIF measurements of agave plants may be used for the early detection of certain types of disease and for determining the effect of a bactericide on bacteria. The results also showed that fluorescence intensity ratios can be used as a reliable indicator of the progress of disease. 相似文献
995.
High throughput functional assays are increasingly relied upon to generate early and novel discovery leads for drug development. Ion transport proteins including channels, transporters, and pumps play central roles in cellular bioenergetics, excitability, and a multitude of other biological functions. Facile, robust methods for detecting ion transport activity in both native and heterologous systems is desirable for rapid functional analysis and drug discovery for these difficult but important targets. Here we discuss cell-compatible fluorescent probes, functional assays, and VIPR instrumentation that are used to monitor real-time target activity and screen large chemical libraries for potent and selective modulators. Advances and issues for both exogenously applied and fluorescent protein probes of cellular membrane potential, Ca2+, Cl-, and pH are addressed. High throughput screening (HTS) compatible, rapid kinetic and fluorescence resonance energy transfer (FRET) assays are emphasized, in particular the use of voltage-sensitive FRET probes to assay ion channel activity in single cells and 96/384-well formats. 相似文献
996.
High-throughput method for the production and analysis of large natural product libraries for drug discovery 总被引:6,自引:0,他引:6
Eldridge GR Vervoort HC Lee CM Cremin PA Williams CT Hart SM Goering MG O'Neil-Johnson M Zeng L 《Analytical chemistry》2002,74(16):3963-3971
High-throughput methods were applied to the production, analysis, and characterization of libraries of natural products in order to accelerate the drug discovery process for high-throughput screening in the pharmaceutical and biotechnology industries. Library production integrates automated flash chromatography, solid-phase extraction, filtration, and high-throughput parallel four-channel preparative high-performance liquid chromatography to obtain the libraries in 96- or 384-well plates. Libraries consist of purified fractions with approximately one to five compounds per well. Libraries are analyzed prior to biological screening by a high-throughput parallel eight-channel liquid chromatography-evaporative light scattering detection-mass spectrometry system to determine the molecular weight, number, and quantity of compounds in a fraction. After biological screening, active fractions are rapidly purified at the microgram level and individual compounds are rescreened for confirmation of activity. Structures of active compounds are elucidated by NMR spectroscopy and mass spectrometry. Utilization of a novel microcoil probe allows NMR data to be gathered on 50 microg. As a demonstration, a library was made from the stem bark of Taxus brevifolia. Biological screening in the National Cancer Institute's in vitro panel of three cancer cell lines demonstrates that the process enables the discovery of active anticancer compounds not detected in the flash fractions from which the library originates. 相似文献
997.
Nine-analyte detection using an array-based biosensor 总被引:4,自引:0,他引:4
Taitt CR Anderson GP Lingerfelt BM Feldstein sM Ligler FS 《Analytical chemistry》2002,74(23):6114-6120
A fluorescence-based multianalyte immunosensor has been developed for simultaneous analysis of multiple samples. While the standard 6 x 6 format of the array sensor has been used to analyze six samples for six different analytes, this same format has the potential to allow a single sample to be tested for 36 different agents. The method described herein demonstrates proof of principle that the number of analytes detectable using a single array can be increased simply by using complementary mixtures of capture and tracer antibodies. Mixtures were optimized to allow detection of closely related analytes without significant cross-reactivity. Following this facile modification of patterning and assay procedures, the following nine targets could be detected in a single 3 x 3 array: Staphylococcal enterotoxin B, ricin, cholera toxin, Bacillus anthracis Sterne, Bacillus globigii, Francisella tularensis LVS, Yersiniapestis F1 antigen, MS2 coliphage, and Salmonella typhimurium. This work maximizes the efficiency and utility of the described array technology, increasing only reagent usage and cost; production and fabrication costs are not affected. 相似文献
998.
The calculated spectral transmittance of a multilayer laser mirror is used to determine the effective index of the single layer equivalent to the multilayer stack. We measure the artificial anisotropy of photoresist thin films whose structure is a one-dimensional, subwavelength grating obtained from interference fringes. The limitation of the theory of the first-order effective index homogenization is discussed. We designed normal-incidence, polarizing coating and a polarization rotator by embedding anisotropic films in simple multilayer structures. 相似文献
999.
Lenoble J Martin T Blumthaler M Philipona R Albold A Cabot T de La Casinière A Gröbner J Masserot D Müller M Pichler T Seckmeyer G Schmucki D Touré ML Yvon A 《Applied optics》2002,41(9):1629-1639
A measurement campaign was organized in March 1999 in the Bavarian Alps as part of the European project, Characteristics of the UV Radiation Field in the Alps (CUVRA), to analyze the effect of altitude, aerosols, and snow cover on ground-level UV spectral irradiance. We present the results of simultaneous measurements of aerosol optical depth (AOD) made at various sites on two cloudless days in March 1999. The two days exhibited different aerosol conditions. Results derived from spectral measurements of UV irradiance are compared with data from filter radiometer measurements made at discrete wavelengths extending from the UV to the near IR. The different methods generated values for the AOD that were in good agreement. This result confirms that one can use either method to retrieve the AOD with an uncertainty of approximately 0.03-0.05. On 18 March, high turbidity was observed at low altitude (400-nm AOD approximately 0.5 at 700 m above sea level), and the AOD decreased regularly with altitude; on 24 March, the turbidity was much less (0.11 at 700 m above sea level). On both days very low AODs (0.05-0.09) were measured at 3000 m above sea level. The spectral dependence of the AOD is often parameterized by the angstrom relationship; the alpha parameter is generally difficult or impossible to retrieve from spectral measurements because of the relatively narrow wavelength range (320-400 nm), and only one of the spectro-radiometers used during the campaign permits this retrieval. In most cases, during this field campaign, alpha was found by filter sunphotometers to be 1.1-1.5. 相似文献
1000.
The oxidation behavior in air of a physical vapor-deposited (PVD) Mg–10.6Zr (wt. %) alloy was studied in the 325–450°C temperature range. The oxidation rate of this alloy remains low at temperatures below 375°C. However, at higher temperatures, the alloy experienced extremely high oxidation rates, which can even lead to disintegration of the sample. Oxidation is controlled by fast inward oxygen transport along the open boundaries of the alloy, leading to the formation of cracks throughout the sample, and subsequent formation of a thin MgO at crack interfaces. The MgO layer remains protective while coarsening of zirconium precipitates at the open boundaries does not take place. Thickening of Zr precipitates over a critical size induces impairment of the MgO layer at crack interfaces, facilitating inward oxygen diffusion. The volume increase resulting from the formation of new oxide at open boundaries favors decohesion of open boundaries, leading to accelerated oxidation. 相似文献