Clinicopathological significance of intratubular giant macrophages in progressive glomerulonephritis

Very large macrophages, which we have termed "giant macrophages" (G-M phi), have been found in renal tubules, some containing cytoplasmic vacuoles. To elucidate their pathophysiological roles, we examined renal biopsy tissues from various primary glomerulonephritis (GN) and tubulointerstitial nephritis (TIN) using immunohistochemistry with monoclonal antibodies against M phi and other cell surface markers. Giant macrophages were absent or rare in TIN, minimal change nephrotic syndrome, and minor glomerular abnormalities, but G-M phi was plentiful in progressive glomerulonephrides such as IgA nephropathy with crescents, membranoproliferative GN, focal segmental glomerulosclerosis, and especially in crescentic GN. These G-M phi were usually seen in the lumen of renal tubules, but occasionally were found in the Bowman's spaces and glomerular tufts, and similar cells were also found in urine. Moreover, they frequently made contact with tubular epithelial cells expressing intercellular adhesion molecule-1, and the tubular epithelial cells in such lesions often had degenerative changes. Giant M phi may damage tubular epithelial cells from the luminal side. Phenotypically, G-M phi showed activated (CD71+) and mature (25F9+) characteristics along with features of M phi (CD68+), and the cytoplasm contained a great deal of lipids. The numbers of G-M phi in renal tissues closely correlated with the degree of hematuria (rho = 0.5, P < 0.001), serum creatinine value (r = 0.63, P < 0.001) in GN patients (N = 96) and with proteinuria in IgA nephropathy patients (r = 0.89, P < 0.001, N = 27). These data suggest that G-M phi are M phi that were activated and matured in certain active inflammatory sites, which flowed into tubules and then into urine. Thus, the existence of G-M phi in biopsy tissue or urine reflect the activity of GN and may have a predictive value for the progression of GN.     

A new quadruple therapy for the eradication of Helicobacter pylori. Effect of pretreatment with omeprazole on the cure rate

The aim of the study was to assess the speech discrimination ability of postlingually deaf adults implanted with the Combi 40 cochlear implant and to compare the results with the postoperative data published for other devices. The postoperative open and closed set speech perception performance of 21 consecutive patients was tested using a standardized test battery comprising a number, monosyllable, sentence, consonant and vowel discrimination test as well as a rhyme test in the sound only condition. Mean values achieved for each test 1, 6 and 12 months after "switch on" were evaluated. The results demonstrate that all patients have a substantial benefit from their implant and show a continuous improvement in their speech perception abilities with increased device experience. The mean percentages of correct answers after 12 months were 93.4 for numbers, 44.6 for monosyllables, 78.5 for sentences, 67.6 for the rhyme test, 59.8 for vowel, and 67.3 for consonant discrimination. Preoperatively, the mean discrimination score for monosyllables was 0%. The speech discrimination scores of our patients were similar or higher than described for similar patient groups implanted with other devices. The high stimulation rate of the implant system using the continuous interleaved speech processing strategy as well as a deep atraumatic electrode insertion into the apicalmost regions of the scala tympani may be the reason for good performance.     

Prediction of starting performance of synchronous motor by DC decaytesting method with the rotor in any arbitrary position

This paper presents a novel method to predict the starting performance of synchronous motors. This proposed strategy is based on a simple standstill response testing method using a small-capacity DC power supply unit, tentatively named the DC decay testing method in this paper, and can be applied to standstill synchronous motors with the rotor in any arbitrary position. The proposed testing method is carried out on a 10 kW, 200 V, 31.9 A, 50 Hz four-pole laminated salient-pole synchronous motor with damper windings. The results measured by on-load test and those predicted by the proposed method on starting performance clearly show the validity of the proposed method     

Nitric oxide decomposition in air by using nonthermal plasmaprocessing with additives and catalyst

Catalyst (copper-coated zeolite catalyst, i.e., Cu-ZSM-5) was used to enhance NO_x removal plasma chemical reactions. Two kinds of hydrocarbons (2-propane-1-ol, 2-propanol) were added to the synthesized flue gas before the nonthermal plasma process, and their effects on NO_x removal characteristics were investigated. Enhancement effects of NO_x removal by the nonthermal plasma process with hydrocarbons as the additives were confirmed. Usually, the catalyst's working temperature is much higher than the room temperature. A catalytic reactor was installed after the plasma reactor. Catalytic effects on NO_x removal characteristics disappeared when the synthesized flue gas temperature was increased (~250°C). When the synthesized flue gas temperature was at room temperature, about 90% NO _x removal efficiency was realized with a combination of hydrocarbons, the catalytic reactor, and the pulsed discharge plasma     

Traceable P2P record exchange: a database-oriented approach

In recent years, peer-to-peer (P2P) technologies are used for flexible and scalable information exchange in the Internet, but there exist problems to be solved for reliable information exchange. It is important to trace how data circulates between peers and how data modifications are performed during the circulation before reaching the destination for enhancing the reliability of exchanged information. However, such lineage tracing is not easy in current P2P networks, since data replications and modifications are performed independently by autonomous peers—this creates a lack of reliability among the records exchanged. In this paper, we propose a framework for traceable record exchange in a P2P network. By managing historical information in distributed peers, we make the modification and exchange histories of records traceable. One of the features of our work is that the database technologies are utilized for realizing the framework. Histories are maintained in a relational database in each peer, and tracing queries are written in the datalog query language and executed in a P2P network by cooperating peers. This paper describes the concept of the framework and overviews the approach to query processing.     

Avoiding Routing Loops on the Internet

Suppose that some particular link in the Internet is currently congested. A natural solution is to try to make packets bypass that link. This can be done by increasing the cost of that link intentionally, say from a ₁ to a ₂, since the Internet uses shortest-path routing. Unfortunately, however, this often causes temporary loops for packet traveling, called routing loops. In this paper we show that routing loops can be avoided by increasing the cost of the link not directly from a ₁ to a ₂ but through an intermediate value, a ₃, i.e., from a ₁ to a ₃ and then to a ₂. We may need several intermediate values. We show that in this case the greedy strategy, namely, raising the cost as much as possible in each step, is optimal.     

Nanowrinkled and Nanoporous Polyethylene Membranes Via Entanglement Arrangement Control

Crystalline homopolymers, including polyethylene (PE), which has the simplest architecture, form a nanometer‐sized combination of crystalline and amorphous components, but their arrangement control, similar to self‐assembled phase‐separation of block‐copolymers, is usually difficult. However, molecular entanglements trapped between crystalline and amorphous components of homopolymers coincide with the segmental linking points on the interfaces of the microphase separation for block copolymers. Nanowrinkled PE membranes are prepared with a network of 30 nm‐thick homogeneous lamellae using a novel entanglement control technique composed of biaxial melt‐drawing and melt‐shrinking procedures, which are limited for highly entangled ultrahigh molecular weight materials. Such a network arrangement of nanowrinkling lamellae spreading on membrane surface and also across the membrane thickness improves the mechanical properties of both tensile strength and tearing strength. Subsequent cold‐drawing causes delamination of the lamellar interfaces, leading to the resultant nanoporous morphology composed of passing‐through channels that are several tens of nanometers in diameter, without any solvent processing.     

Isolation and Characterization of Two Types of Xyloglucanases from a Phytopathogenic Fungus,Verticillium dahliae

Xyloglucan is a major hemicellulosic component in plant cell walls. Phytopathogenic fungi secrete cell wall-degrading enzymes on their infection to hosts, while the nature of the cell wall-lytic enzymes of such fungi are yet to be fully understood. Verticillium dahliae is a soil-borne fungus that causes vascular wilt diseases in a variety of commercially important crops worldwide. We purified two types of xyloglucanases, XEG12A and XEG74B, from the culture of naturally isolated Verticillium dahliae strain 2148. XEG12A showed a molecular size of 23 kDa with its maximal activity at pH 7.5. XEG12A specifically hydrolyzed xyloglucan with no activity on other β-glucans. XEG74B had a molecular size of 110 kDa with its optimum pH at 6.0. XEG74B primarily hydrolyzed xyloglucan, with a slight activity on β-1,3-1,4-glucan. Analysis of hydrolytic products of xyloglucanooligasaccharide (XXXGXXXG) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) revealed that the both enzymes cleaved β-1,4-glucosidic linkage at the position of unbranched chain, while XEG74B showed a little fluctuation with the cleavage site. Both enzymes did not hydrolyzed xyloglucanoheptasaccharide (XXXG) at all. N-Terminal and internal amino acid sequencing of the enzymes revealed that XEG12A and XEG74B belonged to Glycoside Hydrolase (GH) Families 12 and 74, respectively. Based on these results we concluded that V. dahliae XEG12A and XEG74B were xyloglucan-specific endo-β-1,4-glucanases (EC 3.2.1.151).