Folate receptor targeted, carboxymethyl chitosan functionalized iron oxide nanoparticles: a novel ultradispersed nanoconjugates for bimodal imaging

This article delineates the design and synthesis of a novel, bio-functionalized, magneto-fluorescent multifunctional nanoparticles suitable for cancer-specific targeting, detection and imaging. Biocompatible, hydrophilic, magneto-fluorescent nanoparticles with surface-pendant amine, carboxyl and aldehyde groups were designed using o-carboxymethyl chitosan (OCMC). The free amine groups of OCMC stabilized magnetite nanoparticles on the surface allow for the covalent attachment of a fluorescent dye such as rhodamine isothiocyanate (RITC) with the aim to develop a magneto-fluorescent nanoprobe for optical imaging. In order to impart specific cancer cell targeting properties, folic acid and its aminated derivative was conjugated onto these magneto-fluorescent nanoparticles using different pendant groups (-NH(2), -COOH, -CHO). These newly synthesized iron-oxide folate nanoconjugates (FA-RITC-OCMC-SPIONs) showed excellent dispersibility, biocompatibility and good hydrodynamic sizes under physiological conditions which were extensively studied by a variety of complementary techniques. The cellular internalization efficacy of these folate-targeted and its non-targeted counterparts were studied using a folate-overexpressed (HeLa) and a normal (L929 fibroblast) cells by fluorescence microscopy and magnetically activated cell sorting (MACS). Cell-uptake behaviors of nanoparticles clearly demonstrate that cancer cells over-expressing the human folate receptor internalized a higher level of these nanoparticle-folate conjugates than normal cells. These folate targeted nanoparticles possess specific magnetic properties in the presence of an external magnetic field and the potential of these nanoconjugates as T(2)-weighted negative contrast MR imaging agent were evaluated in folate-overexpressed HeLa and normal L929 fibroblast cells.     

Synergy effects of hybrid carbon system on properties of composite bipolar plates for fuel cells

A hybrid carbon system of graphite powder (GP) and continuous carbon fibre fabric (CFF) is used for an epoxy composite to improve the electrical conductivity, mechanical properties and mouldability of a composite bipolar plate. These improvements are achieved simultaneously by inserting several layers of CFF into the GP/epoxy composite to enhance the mechanical properties and in-plane conductivity. The electrical properties, flexural strength and mouldability of the composite plates are measured as a function of conducting filler content and number of CFF layers. The composites show improved electrical conductivity, flexural properties and mouldability. Composites with 70-75 vol.% carbon fillers have the highest electrical conductivity with reasonable flexural properties. These results suggest that the poor mouldability and low through-plane electrical conductivity of the continuous fibre composite bipolar plate, as well as the weak flexural properties of GP composites, can be overcome by incorporating a GP/CFF hybrid system.     

Non-uniform illumination in concentrating solar cells

After a gap of more than two decades, Concentrator Photovoltaics (CPV) technology is once again under spotlight for making use of the best available solar cell technologies and improving the overall performance. CPV finds its use in a number of applications ranging from building integration to huge power generation units. Although the principles of solar concentration are well understood, many practical design, operation, control issues require further understanding and research. A particular issue for CPV technology is the non-uniformity of the incident flux which tends to cause hot spots, current mismatch and reduce the overall efficiency of the system. Understanding of this effect requires further research, and shall help to employ the most successful means of using solar concentrators. This study reviews the causes and effects of the non-uniformity in the CPV systems. It highlights the importance of this issue in solar cell design and reviews the methods for the solar cell characterization under non-uniform flux conditions. Finally, it puts forward a few methods of improving the CPV performance by reducing the non-uniformity effect on the concentrator solar cells.     

Photon and vibration synergism on planar defects induced 2D-graphitic carbon nitride for ultrafast remediation of dyes and antibiotic ampicillin

Journal of Materials Science - Hybrid oxidation methodologies (HOMs) and active site enrichment of 2D nanocatalyst through defects induction are ubiquitously used for generating adequate reactive...     

Segmentation of breast lesion in DCE-MRI by multi-level thresholding using sine cosine algorithm with quasi opposition-based learning

Pattern Analysis and Applications - In recent times, the high prevalence of breast cancer in women has increased significantly. Breast cancer diagnosis and detection employing computerized...     

Polyaniline-Acetylene black-Copper cobaltite based ternary hybrid material with enhanced electrochemical properties and its use in supercapacitor electrodes

A new system based on Polyaniline-Acetylene black-Copper cobaltite composite has been prepared and affirmed by XRD, UV, SEM, FTIR, and EDS characterizations. The rod-like texture of ternary hybrid system offered excellent electrochemical activity in comparison to single and binary systems. CV and CD results revealed outstanding redox behavior of the ternary hybrid electrodes. Ternary electrode (PACC) presented the highest specific capacitance value of 690 F/g at 1 mA/cm² current density. PACC electrode based symmetric supercapacitor had a specific capacitance of 137.25 Fg^-1 at 1 mA/cm² of current density. PACC symmetric supercapacitor had the highest specific power and specific energy of 3308.85 W.kg^-1 and 19.064 Wh.kg^-1, respectively. The ternary system provides less charge transfer resistance values compared to all other systems. Thermal stability of the ternary composite is way better than polyaniline, which is due to the contribution of copper cobaltite and acetylene black. The overwhelming characteristics of the ternary hybrid composite bring it to the limelight as an excellent candidate in the field of supercapacitors.     

Characterisation of a Schottky ISFET as Hg-MOSFET and as cytochrome P450-ENFET

A cost-effective and simple method is proposed wherein a Schottky ion sensitive field effect transistor (Schottky ISFET)-based sensor is characterised as metal oxide semiconductor and enzyme field effect transistor (ENFET). This technique involves deposition of mercury (Hg) as gate material over the sensing layer mitigating the complexity of fabrication process, thereby eliminating the need of refabricating an identical device. A Schottky-based ISFET simplifies the fabrication process as the requisite for doping of source and drain regions becomes redundant. Steps involved in lithography process for fabricating metal oxide semiconductor field effect transistor (MOSFET) are reduced with the use of liquid metal Hg as gate over layer. Such a device can be transformed back to an ISFET without any additional etching process. Furthermore, the same ISFET device can be utilised as an ENFET when the former is used in conjunction with a biological element. In this work, a Schottky-based ISFET has been characterised as Hg-MOSFET and as cytochrome P450-ENFET. Multiple tests on the device exhibit that the same ISFET sensor can be used both as a MOSFET and an ENFET with good repeatability and versatility without losing its sensitivity.     

From Mountain Ranges to Sweeping Plains,in Droughts and Flooding Rains; River Murray Water Quality over the Last Four Decades

The aim of this paper was to analyse the spatial and temporal patterns and drivers of water quality in a large arid/semi-arid river system (River Murray, Australia) using a long term (1978???2015) dataset collected from 24 monitoring sites. The water quality is highly variable, but on average electrical conductivity (EC), pH, turbidity, dissolved and total nutrient, colour and chlorophyll a levels increase with distance downstream from the headwaters to the lower reaches. This is a function of the natural accumulation of dissolved and particulate components and intermittent, mostly diffuse source, pollutant inputs. The Darling tributary inflow increases turbidity, total phosphorus and pH in the main River Murray channel. Based on long-term trend analysis at four representative sites, EC, nutrients and colour showed declining trends on average at most sites except in the headwaters. Increased flow increases concentrations of most quality parameters, although at very high flows decreases in pH, EC, turbidity and oxidized nitrogen were apparent at many sites. The extreme “Millennium” drought (2002???2009) period resulted in lowered concentrations of many water quality parameters, indicating retention in the landscape. In the post-drought flooding (2010???2012) period a large amount of organic material was mobilised, resulting in much higher peak colour concentrations than when mid-range flooding was more frequent. It is critical that this monitoring program is continued as a Basin-wide water management plan is implemented.

     

Estimation of riverbank soil erodibility parameters using genetic algorithm

Determination of the erodibility parameters, such as critical shear stress and erodibility coefficient, are necessary before estimating the annual bank erosion (or bank retreat) at river reaches. However, in many cases, the river site is inaccessible making it difficult to assess the soil parameters either by in situ tests or by laboratory experiments. In this study, Genetic Algorithm (GA)-based optimisation technique was used to estimate the erodibility parameters of middle reaches of the Brahmaputra River in India. Two approaches were followed. At first, erodibility parameters were estimated using daily stage records at a selected site. Secondly, based on the annual observed bank erosions (bank retreat) from satellite images, erodibility parameters were estimated in three different river reaches. All these results were compared with that from a previous study using in situ jet tests. Annual bank erosions (bank retreat) were estimated using the median values of the erodibility parameters. The results agree well with the average observed annual bank erosion of these river reaches. In addition, the effects of measurement errors and optimisation algorithms on the parameter estimation were analysed. Sensitivity analysis of the parameters in GA was evaluated and it was found that GA can be utilised in the data-scarce regions to estimate the average erodibility parameters.