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131.
SA Marzouk VV Cosofret RP Buck H Yang WE Cascio SS Hassen 《Canadian Metallurgical Quarterly》1997,69(14):2646-2652
In this paper, fabrication, characterization, and physiological application of a miniaturized amperometric lactate biosensor are described. The sensor is based on cross-linked lactate oxidase and tetrathiafulvalene-tetracyano-quinodimethane (TTF-TCNQ) charge transfer complex. The sensor was developed for continuous quantitative measurement of the lactate accumulation in ischemic myocardium under severe depletion of oxygen. The sensor was evaluated in vitro at an applied potential of 0.15 V vs Ag/AgCl; it proved to combine all the performance characteristics desired for the present application, such as proper response in absence of oxygen, good operational stability, good accuracy and precision (103.5 +/- 1.2%), adequate response time (t95% = 80 s), and wide linear dynamic range up to 27 mM (r = 0.9998) in N2-saturated solutions and at 37 degrees C. The prepared sensors (n = 12) showed sensitivity of 380 +/- 90 nA/mM, and a background current of 240 +/- 50 nA. The lower limit of detection is 0.4 +/- 0.15 mM with a S/N ratio equal to 3. Results obtained for direct lactate monitoring in ischemic rabbit papillary muscle under no-flow conditions and PO2 < 6 mm Hg are presented. 相似文献
132.
VV Kukharchuk EIu Solov'ev PP Malyshev TA Rozhkova OA Semenova EE Aronskaia 《Canadian Metallurgical Quarterly》1997,69(9):41-45
13 patients aged 39 to 60 years with coronary atherosclerosis confirmed at selective coronary angiography combined with primary hyperlipidemia (phenotypes 2a and 2b) received enduracin in a dose 1500 mg/day. As a result of the treatment total cholesterol (TC) and LDL cholesterol lowered by 10.3 and 13.1%, respectively, whereas HDL cholesterol rose by 15.2%. Half of the patients demonstrated activation of hepatic transaminases, but discontinuation of the drug was not necessary. In 3 out of 4 patients after 2 years of enduracin treatment stabilization of atherosclerosis was observed. Thus, long-term enduracin is able to inhibit progression of atherosclerosis in coronary heart disease patients. 相似文献
133.
AG Kats VV Afanas'ev IaM Biberman IF Romacheva VS Starodubtsev MB Shvyrkov 《Canadian Metallurgical Quarterly》1998,77(4):67-68
Analysis of the results of examinations in oral surgery making use of tests in 319 fourth-year students showed that such a method of assessing students' knowledge is highly objective. 相似文献
134.
Leydig cell tumors are very rarely seen testicular tumors and can be difficult to distinguish from testicular tumors of the adrenogenital syndrome. Testicular tumors of the adrenogenital syndrome are confined to patients with congenital adrenal hyperplasia. The authors report a case of a patient with malignant Leydig cell tumor and a history of congenital adrenal hyperplasia (adrenogenital syndrome). To the authors' knowledge, this has not been reported previously. 相似文献
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137.
SM VanRheenen X Cao VV Lupashin C Barlowe MG Waters 《Canadian Metallurgical Quarterly》1998,141(5):1107-1119
SEC35 was identified in a novel screen for temperature-sensitive mutants in the secretory pathway of the yeast Saccharomyces cerevisiae (. Genetics. 142:393-406). At the restrictive temperature, the sec35-1 strain exhibits a transport block between the ER and the Golgi apparatus and accumulates numerous vesicles. SEC35 encodes a novel cytosolic protein of 32 kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec35-1 is efficiently suppressed by YPT1, which encodes the rab-like GTPase required early in the secretory pathway, or by SLY1-20, which encodes a dominant form of the ER to Golgi target -SNARE-associated protein Sly1p. Weaker suppression is evident upon overexpression of genes encoding the vesicle-SNAREs SEC22, BET1, or YKT6. The cold-sensitive lethality that results from deleting SEC35 is suppressed by YPT1 or SLY1-20. These genetic relationships suggest that Sec35p acts upstream of, or in conjunction with, Ypt1p and Sly1p as was previously found for Uso1p. Using a cell-free assay that measures distinct steps in vesicle transport from the ER to the Golgi, we find Sec35p is required for a vesicle docking stage catalyzed by Uso1p. These genetic and biochemical results suggest Sec35p acts with Uso1p to dock ER-derived vesicles to the Golgi complex. 相似文献
138.
NE Ordian AI Va?do VV Rakitskaia NV Shiriaeva FI Pro?mina NG Lopatina VG Shaliapina 《Canadian Metallurgical Quarterly》1998,125(4):443-445
Although behavioral and neuropsychological data regarding the existence of images for odors are inconclusive, reconsideration of earlier EEG work provides reasonably clear evidence for an inner nose. However, further EEG studies and neuroimaging data seem essential for conclusive demonstration of an inner nose. 相似文献
139.
Workers engaged into subsurface extraction of sand containing gold were proved to work in difficult conditions characterized by stable negative temperatures, high concentrations of dust, elevated levels of noise and vibration. The drill operators demonstrated extremely unfavorable changes of physiologic processes within a shift. Those changes were compromised cardiovascular regulation, early occurrence and intensive progress of fatigue. The authors provide recommendations to normalize the work conditions and to create rational scheme of work and rest for the occupations. 相似文献
140.
While complex examination of patients with megaureter in late stages it was established the afection in the first place of the ureter's muscular layer and the disorder of its contractice capacity. The causes of megaureter's occurrence were: obstruction of the ureter's intramural portion and vesico-ureteric reflux. Longitudinal resection of megaureter was conducted in 25 patients, the longitudinal ureter's fold formation--in 6, intraureteric plasty was done in 28. In 93% of observations the intraureteric plasty efficacy was noted. 相似文献