新一代厂站计算机监控系统软件的研制

本介绍一种新的基于开放系统环境的计算机监控系统软件的开发研究及其设计目标、实现方法及应用情况。     

Efficient random subcloning of DNA sheared in a recirculating point-sink flow system

Based on a high-performance liquid chromatographic pump, we have built a device that allows recirculation of DNA through a 63-microm orifice with ensuing fractionation to a minimum fragment size of approximately 300 base pairs. Residence time of the DNA fragments in the converging flow created by a sudden contraction was found to be sufficiently long to allow extension of the DNA molecules into a highly extended conformation and, hence, breakage to occur at midpoint. In most instances, 30 passages sufficed to obtain a narrow size distribution, with >90% of the fragments lying within a 2-fold size distribution. The shear rate required to achieve breakage was found to be inversely proportional to the 1.0 power of the molecular weight. Compared with a restriction digest, up to 40% of all fragments could be cloned directly, with only marginal improvements in cloning efficiency having been observed upon prior end repair with Klenow, T4 polymerase or T4 polynucleotide kinase. Sequencing revealed a fairly random distribution of the fragments.     

铌酸锂γ射线辐照色心缺陷的研究

对铌酸锂晶体进行γ射线辐照处理，吸收光谱显示基础吸收边紫移，４８０ｎｍ波长附近出现一明显吸收峰，这是由空位色心Ｖ０和Ｎｂ＾４＋缺陷引起的，Ｎｂ＾４＋的存在进一步被ＥＰＲ实验所证实。本文对上述辐照缺陷的产生机理进行了讨论。     

微机监控技术 一

本文系统地讲述了以微型计算机为中心的微机监控系统的工作原理、系统组成和应用方面的有关技术。分为监控系统的功能和结构、硬件系统和软件系统三个部分。本文为第一部分。     

The band areas of proteins determined by fluorescent scanning in the commercial automated gel electrophoresis apparatus

An automated gel electrophoresis apparatus, recently available commercially, allows one to follow the band during electrophoresis in real time, and lends itself therefore to an evaluation of bandwidth as a function of migration time (the dispersion coefficient), resolution and band shape. These determinations assume the constancy of band area with migration time and at various gel concentrations. The purpose of the present study was to verify these assumptions. Representative proteins and sodium dodecyl sulfate (SDS)-proteins, either natively fluorescent or fluorescein carboxylate labeled, were found to exhibit band areas which approach constancy as a function of migration time in both agarose and polyacrylamide gel electrophoresis, provided that (i) the protein concentration under the band was low enough to obviate self-quenching of fluorescence; (ii) the separation of the protein of interest from contaminants had progressed sufficiently during the time at which band areas were measured; (iii) the baseline under the peak was sufficiently well defined. However, band areas decrease with increasing gel concentration. Protein peaks exhibited leading and trailing tails. The ratio of the combined tail area to total area appeared to be near-constant at varying migration times. However, that ratio increases with increasing gel concentration. The tail area does not appear to be an artifact of fluorometric detection since it is reproduced upon fluorimetric analysis of the protein eluted from gel slices after electrophoresis. However, it may be due to photochemical destruction under the conditions of repetitive fluorometric peak detection.     

A Built-in Self-Test Scheme with Diagnostics Support for Embedded SRAM

In this paper we propose a novel built-in self-test (BIST) design for embedded SRAM cores. Our contribution includes a compact and efficient BIST circuit with diagnosis support and an automatic diagnostic system. The diagnosis module of our BIST circuit can capture the error syndromes as well as fault locations for the purposes of repair and fault/failure analysis. In addition, our design provides programmability for custom March algorithms with lower hardware cost. The combination of the on-line programming mode and diagnostic system dramatically reduces the effort in design debugging and yield enhancement. We have designed and implemented test chips with our BIST design. Experimental results show that the area overhead of the proposed BIST design is only 2.4% for a 128 KB SRAM, and 0.65% for a 2 MB one.     

试论降低成本的设计技术

一个新产品的功能和成本水平基本上已在产品设计阶段就确定了。研究降低成本的设计技术就是为了给设计师提供一套行之有效的降低和优化成本的辅助设计工具。降低成本设计的技术内涵是与设计过程并行的成本估算、知识基系统的建立和质量及成本的综合优化。论述了各国对此项技术研究应用的状况、技术分析、发展特点和研究前沿的项目及内容 ,如对此技术研究应用的迫切性、对降低成本的最佳时机和产品的全成本概念等的探讨和认识 ;对成本扩展到产品寿命全周期、对目标成本控制法和成本优化设计等的研究和应用。     

国外农用非织造布覆盖材料的应用

非织造布在现代农业生产中占有重要地位 ,非织造布作为农用覆盖材料在柑橘、草莓、茶叶、花卉、水稻和玉米育苗以及人参栽培等多方面应用较为广泛 ,本文对国外农用非织造布覆盖材料的特点、性能以及应用情况作了介绍     

一种在Kalman滤波中引入径向速度测量的新方法

研究在距离和径向速度测量噪声统计相关的情形下把径向速度测量引入Kalman滤波的新方法。分析了径向速度测量噪声与位置测量更新后状态滤波误差的统计相关性,根据Gauss-Markov定理导出了对应于径向速度测量的滤波方程由此而得到一种序贯滤波算法。两个不同的蒙特卡罗仿真表明,通过采用这一新算法引人径向速度测量,不仅可以大大提高状态估计的精度,而且其估计性能和计算效率优于传统的EKF。