Residence time distribution of polymer melt in the T-die

Theoretical or rheological calculations for crosshead die geometry were thought not worthwhile until recently, and restrictor, or choker, bars were often excessively relied upon for film uniformity. Thus, the residence time distribution of a polymer melt was infrequently calculated especially in the T-die, because it was assumed to be very wide in T-dies. This report provides a general equation expressing the residence time distribution of polymer melts in T-dies, and indicates how to take an optimum combination of the flow-path dimensions in order to obtain both a high flow uniformity and a comparatively narrow residence time distribution across the die width. Such a T-die designed by the above considerations will produce a shorter heat history and improved physical properties of the sheet or film produced.     

Dynamic characteristics and dual control of a ball screw drive with integrated piezoelectric actuator

This paper describes the precision continuous path tracking control by using a dual-actuated single stage. First, fine-drive mechanism and the dynamic model of the entire drive system are described. In the simulation model, the dynamic characteristic of the dual-actuated stage is investigated to see whether it can provide precise motion by using dual control. Second, the fine motion controller is designed. Adjusting the control parameters, a positioning resolution of 20 nm and a bandwidth of 260 Hz were obtained. Third, the frequency responses of coarse and fine drives are experimentally investigated. After that, the dual controller is designed based on the investigated dynamics. Finally, whether coarse motion and fine motion could work complimentarily by the dual servo is examined in the experiments. By using the simultaneous dual controller, tracking errors were reduced sufficiently compared to the single coarse control.     

Micro–macro simulation of sintering process by coupling Monte Carlo and finite element methods

A micro–macro method for simulating a sintering process of ceramic powder compacts based on the Monte Carlo and finite element methods is proposed. Macroscopic non-uniform shrinkage during the sintering is calculated by the viscoplastic finite element method. In the microscopic approach using the Monte Carlo method, powder particles and pores among the particles are divided into many cells, and the growth of grains in the particles and the disappearance of pores are simulated by means of the Potts model.The shrinkage strain rate required as a materials constant in the macroscopic method is calculated by the microscopic approach. The microscopic and macroscopic approaches are coupled by exchanging microscopic and macroscopic results in each finite element step. In the Monte Carlo method, the effect of macroscopic viscoplastic deformation on the microstructural change is taken into consideration by including viscoplastic strain rate calculated by the finite element method in the disappearance frequency of pore cells. The shrinkage behaviour in the sintering of circular two-layer compacts is simulated by the proposed micro–macro method.     

Development of a Millimeter-Wave Electron-Spin-Resonance Measurement System for Ultralow Temperatures and Its Application to Measurements of Copper Pyrazine Dinitrate

We have developed a millimeter-wave electron-spin-resonance (ESR) measurement system using a ³He-⁴He dilution refrigerator for the ultralow-temperature range below 1 K. The currently available frequency range is 125–130 GHz. This system is based on a Fabry-Pérot-type resonator (FPR) that is composed of two mirrors. The frequency can be changed by adjusting the distance between the mirrors using a piezoelectric actuator installed at the bottom of the resonator. A homodyne detection system with an InSb detector is built into the low-temperature section of the ³He-⁴He dilution refrigerator; this system provides high sensitivity. Using this system, we performed ESR measurements on a Heisenberg quantum-spin chain—copper pyrazine dinitrate, Cu(C₄H₄N₂)(NO₃)₂—over the temperature range from 6.6 down to 0.25 K. The ESR lines change continuously with decreasing temperature. Our results suggest that the ESR spectrum of copper pyrazine dinitrate may be useful as a temperature sensor for the very low-temperature range.     

On the treatments of heterogeneities and periodic boundary conditions for isogeometric homogenization analysis

The treatments of heterogeneities and periodic boundary conditions are explored to properly perform isogeometric analysis (IGA) based on NURBS basis functions in solving homogenization problems for heterogeneous media with omni‐directional periodicity and composite plates with in‐plane periodicity. Because the treatment of the combination of different materials in IGA models is not trivial especially for periodicity constraints, the first priority is to clearly specify points at issue in the numerical modeling, or equivalently mesh generation, for IG homogenization analysis (IGHA). The most awkward, but important issue is how to generate patches for NURBS representation of the geometry of a rectangular parallelepiped unit cell to realize appropriate deformations in consideration of the convex‐hull property of IGA. The issue arises from the introduction of overlapped control points located at angular points in the heterogeneous unit cell, which must satisfy multiple point constraint (MPC) conditions associated with periodic boundary conditions (PBCs). Although two measures may be conceivable, we suggest the use of multiple patches along with double MPC that imposes PBCs and the continuity conditions between different patches simultaneously. Several numerical examples of numerical material and plate tests are presented to demonstrate the validity of the proposed strategy of IG modeling for IGHA. Copyright © 2016 John Wiley & Sons, Ltd.     

Fluorescent imaging of endothelial glycocalyx layer with wheat germ agglutinin using intravital microscopy

Endothelial glycocalyx (GCX) is located on the apical surface of vascular endothelial cells and is composed of a negatively‐charged network of proteoglycans and glycoproteins. The GCX plays an important role in maintaining the integrity of vascular walls and preventing leakage of plasma. Therefore, degradation of the GCX is believed to lead to pathological leakage of plasma. Because the GCX is a very thin layer, its ultrastructural image has been demonstrated on electron microscope. To explore the function of the GCX, it should be visualized by a microscope in vivo. Thus, we developed in vivo visualization technique of the GCX under fluorescence microscopy using a mouse dorsal skinfold chamber (DSC) model. To label and visualize the GCX, we used fluorescein isothiocyanate (FITC)‐labeled lectin, which has a high specificity for sugar moieties. We examined the affinity of the different lectins to epivascular regions under an intravital fluorescent microscope. Among seven different lectins we examined, FITC labeled Triticum vulgaris (wheat germ) agglutinin (WGA) delineated the GCX most clearly. Binding of WGA to the GCX was inhibited by chitin hydrolysate, which contained WGA‐binding polysaccharide chains. Furthermore, the septic condition attenuated this structure, suggesting structural degradation of endothelial GCX layer. In conclusion, FITC‐labeled WGA lectin enabled visualization of endothelial GCX under in vivo fluorescence microscopy. Microsc. Res. Tech. 79:31–37, 2016. © 2015 Wiley Periodicals, Inc.     

An approach to mobile software robots for the WWW

This paper describes a framework for developing mobile software robots by using the PLANET mobile object system, which is characterized by a language-neutral layered architecture, the native code execution of mobile objects, and asynchronous object passing. We propose an approach to implementing mobile Web search robots that takes full advantage of these characteristics, and we base our discussion of its effectiveness on experiments conducted in the Internet environment. The results show that the PLANET approach to mobile Web search robots significantly reduces the amount of data transferred via the Internet and that it enables the robots to work more efficiently than the robots in the conventional stationary scheme whenever nontrivial amounts of HTML files are processed     

Comparing the Osteogenic Potential and Bone Regeneration Capacities of Dedifferentiated Fat Cells and Adipose-Derived Stem Cells In Vitro and In Vivo: Application of DFAT Cells Isolated by a Mesh Method

Background: We investigated and compared the osteogenic potential and bone regeneration capacities of dedifferentiated fat cells (DFAT cells) and adipose-derived stem cells (ASCs). Method: We isolated DFAT cells and ASCs from GFP mice. DFAT cells were established by a new culture method using a mesh culture instead of a ceiling culture. The isolated DFAT cells and ASCs were incubated in osteogenic medium, then alizarin red staining, alkaline phosphatase (ALP) assays, and RT-PCR (for RUNX2, osteopontin, DLX5, osterix, and osteocalcin) were performed to evaluate the osteoblastic differentiation ability of both cell types in vitro. In vivo, the DFAT cells and ASCs were incubated in osteogenic medium for four weeks and seeded on collagen composite scaffolds, then implanted subcutaneously into the backs of mice. We then performed hematoxylin and eosin staining and immunostaining for GFP and osteocalcin. Results: The alizarin red-stained areas in DFAT cells showed weak calcification ability at two weeks, but high calcification ability at three weeks, similar to ASCs. The ALP levels of ASCs increased earlier than in DFAT cells and showed a significant difference (p < 0.05) at 6 and 9 days. The ALP levels of DFATs were higher than those of ASCs after 12 days. The expression levels of osteoblast marker genes (osterix and osteocalcin) of DFAT cells and ASCs were higher after osteogenic differentiation culture. Conclusion: DFAT cells are easily isolated from a small amount of adipose tissue and are readily expanded with high purity; thus, DFAT cells are applicable to many tissue-engineering strategies and cell-based therapies.     

Geometry design of a coat-hanger die with uniform flow rate and residence time across the die width

This article provides an approximate but sufficiently practical method of geometry design of a coat-hanger die having both uniform flow rate and residence time across the full width of the die. Such a die is often needed when a heat-sensitive resin such as poly(vinyl chloride) is sheeted, because different residence times across the die width are apt to cause a sheet defect in the transverse direction of the sheet. Although some assumptions were made facilitating mathematical analysis, an application of this method to a sheeting die 1 m wide gave good uniformity of flow rate and residence time.