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91.
Kazuto Ogawa Nao Takekawa Kenko Uchida Shigenobu Shibata 《Asian journal of control》2006,8(3):281-289
We develop an approach to robustness analysis of circadian rhythms from the viewpoints of (local) robust stability of periodic trajectories and their period‐sensitivity, based on mathematical models. Our attention is directed to the core molecular model generating circadian rhythms in Drosophila, proposed by Goldbeter [1], for our approach to be illustrated. We consider two cases when the circadian rhythms are subjected to periodic forcing by light/dark (LD) cycles and when the circadian rhythms are under continuous darkness (DD). First, we propose conditions/algorithms for (local) robust stability, and show that the circadian rhythms generated by the core molecular model are (locally) robustly stable, in both LD and DD cases. Second, in DD case, we derive a formula of period sensitivity, and assess effects of (infinitesimal) parameter‐variations on the period of the circadian rhythms. 相似文献
92.
This paper presents analysis of the recently proposed modulated Hebb-Oja (MHO) method that performs linear mapping to a lower-dimensional subspace. Principal component subspace is the method that will be analyzed. Comparing to some other well-known methods for yielding principal component subspace (e.g., Oja's Subspace Learning Algorithm), the proposed method has one feature that could be seen as desirable from the biological point of view-synaptic efficacy learning rule does not need the explicit information about the value of the other efficacies to make individual efficacy modification. Also, the simplicity of the "neural circuits" that perform global computations and a fact that their number does not depend on the number of input and output neurons, could be seen as good features of the proposed method. 相似文献
93.
Kitamura Y Uzawa T Oka K Komai Y Ogawa H Takizawa N Kobayashi H Tanishita K 《Analytical chemistry》2000,72(13):2957-2962
Nitric oxide (NO) is a gaseous mediator involved in various physiological phenomena, such as vasorelaxation and neurotransmission. Investigation of local cellular responses of NO production in vivo and in vitro requires a measurement method with a high spatial resolution. For selective NO measurement, we therefore developed a microcoaxial electrode whose tip diameter is less than 10 microm. Calibration using various concentrations of NO (0.1-1.0 microM) showed that the electrode has good linearity (r = 0.99) and its detection limit is 0.075 microM (S/N = 3). We verified the applicability of this electrode to in vivo and in vitro local measurement NO released from bovine aortic cultured endothelial cells (BAECs) stimulated by acetylcholine (ACh). After the addition of ACh, a transient increase in NO concentration was detected by the electrode. In the presence of NG-nitro-L-arginine methyl ester (L-NAME), a putative NO synthase inhibitor, NO release (peak NO concentration) from RAECs was significantly less than that in the absence of L-NAME (0.18 +/- 0.04 microM vs 0.47 +/- 0.13; P < 0.01). After removal of L-NAME, NO release partially recovered (0.39 +/- 0.10 microM). In conclusion, the microcoaxial electrode was successfully applied to direct and continuous NO measurement in biological systems. 相似文献
94.
Kobayashi H Shimota A Kondo K Okumura E Kameda Y Shimoda H Ogawa T 《Applied optics》1999,38(33):6801-6807
The interferometric monitor for greenhouse gases (IMG) was the precursor of the high-resolution Fourier-transform infrared radiometer (FTIR) onboard a satellite for observation of the Earth. The IMG endured the stress of a rocket launch, demonstrating that the high-resolution, high-throughput spectrometer is indeed feasible for use onboard a satellite. The IMG adopted a newly developed lubricant-free magnetic suspension mechanism and a dynamic alignment system for the moving mirror with a maximum traveling distance of 10 cm. We present the instrumentation of the IMG, characteristics of the movable mirror drive system, and the evaluation results of sensor specifications during space operation. 相似文献
95.
Mucin-producing tumor of the pancreas: diagnostic value of diffusion-weighted echo-planar MR imaging
Y Yamashita T Namimoto K Mitsuzaki J Urata T Tsuchigame M Takahashi M Ogawa 《Canadian Metallurgical Quarterly》1998,208(3):605-609
It is generally accepted that hemifacial spasm (HFS) and trigeminal neuralgia are caused by compression of the facial nerve (seventh cranial nerve) or the trigeminal nerve (fifth cranial nerve) at the nerve's root exit (or entry) zone (REZ); thus, neurosurgeons generally perform neurovascular decompression at the REZ. Neurosurgeons tend to ignore vascular compression at distal portions of the seventh cranial nerve, even when found incidentally while performing neurovascular decompression at the REZ of that nerve, because compression of distal portions of the seventh cranial nerve has not been regarded as a cause of HFS. Recently the authors treated seven cases of HFS in which compression of the distal portion of the seventh cranial nerve produced symptoms. The anterior inferior cerebellar artery (AICA) was the offending vessel in five of these cases. Great care must be taken not to stretch the internal auditory arteries during manipulation of the AICA because these small arteries are quite vulnerable to surgical manipulation and the patient may experience hearing loss postoperatively. It must be kept in mind that compression of distal portions of the seventh cranial nerve may be responsible for HFS in cases in which neurovascular compression at the REZ is not confirmed intraoperatively and in cases in which neurovascular decompression at the nerve's REZ does not cure HFS. Surgical procedures for decompression of the distal portion of the seventh cranial nerve as well as decompression at the REZ should be performed when a deep vascular groove is noticed at the distal site of compression of the nerve. 相似文献
96.
97.
Tangential velocity change with time of polyacrylamide solution, which had not only viscosity but also elasticity, in a concentric annulus after step motion of the inner cylinder was measured by using a LDV method. By adapting a conception of a second order lag control system which had a feedback connection, an equation for estimating the velocity change with time was presented. Each parameter in the equation was determined based on the experimental results, and the relationships between each parameter and viscosity or elasticity of the solutions was made clear. By using this equation, the tangential velocity change with time of a viscoelastic liquid in the annulus can be estimated when the viscosity and elasticity of the liquid and the rotational speed of the inner cylinder are given. Additionally, it was confirmed that the equation was applicable to the case of polyethyleneoxide solution, which had different ranges of the viscosity and elasticity from those of the test solutions described above. 相似文献
98.
H Iida Y Narita H Kado A Kashikura S Sugawara Y Shoji T Kinoshita T Ogawa S Eberl 《Canadian Metallurgical Quarterly》1998,39(1):181-189
The pyruvate dehydrogenase complex (PDC) plays a key role in the anaerobic mitochondrial metabolism of the parasitic nematode Ascaris suum. A cDNA coding for an A. suum pyruvate dehydrogenase kinase (APDK) has been cloned and sequenced from poly(A)+ RNA isolated from adult A. suum muscle.2 APDK exhibited significant sequence identity to mammalian PDKs. Nucleotide sequence analysis of the APDK cDNA revealed a 22-nucleotide spliced leader, characteristic of many nematode mRNAs, a 5'-UTR of 6 nucleotides, an open reading frame of 1197 nucleotides, and a 3'-UTR of 101 nucleotides that included a putative polyadenylation signal. The open reading frame predicted a protein of 399 amino acids with a molecular weight of 45,402 that included a putative 18-aminoacid leader peptide. Recombinant APDK (rAPDK) was functionally expressed in Escherichia coli with a his tag at its N-terminus and purified to apparent homogeneity on Ni-NTA-agarose. Recombinant APDK was a dimer and was not autophosphorylated and its activity was stimulated in the presence of APDK-deficient adult A. suum muscle PDC presumably by the binding of APDK to the dihydrolipoyl transacetylase (E2) core of the complex. After binding to the core, rAPDK activity was stimulated by elevated NADH/NAD+ and acetyl CoA/CoA ratios within the same ranges as observed for the native APDK. Immunoblotting suggested that native APDK focused as a series of 43-kDa spots (pI 6.1-6.8) on two-dimensional gels of the purified adult A. suum muscle PDC. 相似文献
99.
100.
Y Matsushita K Yoshiie Y Imamura H Ogawa H Imamura S Takao S Yonezawa T Aikou I Maruyama E Sato 《Canadian Metallurgical Quarterly》1998,127(1-2):195-201
Inside-out vesicles of plasma membranes prepared from a plant source were used as models to investigate effects of centrifugal forces on separations of early and late endosome populations by aqueous two-phase partition. Endosome subpopulations were resolved readily by preparative free-flow electrophoresis where acidification of the interiors of late endosomes occurred upon addition of ATP to activate a proton translocating ATPase. The resultant increased diffusion potential provided for a surface difference between late and early endosomes to permit electrophoretic separation. With the plant membranes, unincubated inside-out plasma membrane vesicles modeled early endosomes, whereas inside-out vesicles incubated with 1 mM ATP modeled late endosomes. A latent, 2,4-dichlorophenoxyacetic acid (2,4-D)-(auxin)-stimulated NADH:protein disulfide reductase measured spectrophotometrically was used as an enzymatic marker for both populations of inside-out vesicles. Phase partition behavior of each population was quantitated using total protein as the parameter. 相似文献