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21.
Anna Siskova Jan Kral Jana Drabova Klara Cervena Kristyna Tomasova Jiri Jungwirth Tomas Hucl Pavel Kohout Sandra Summerova Ludmila Vodickova Pavel Vodicka Veronika Vymetalkova 《International journal of molecular sciences》2022,23(14)
A colorectal adenoma, an aberrantly growing tissue, arises from the intestinal epithelium and is considered as precursor of colorectal cancer (CRC). In this study, we investigated structural and numerical chromosomal aberrations in adenomas, hypothesizing that chromosomal instability (CIN) occurs early in adenomas. We applied array comparative genomic hybridization (aCGH) to fresh frozen colorectal adenomas and their adjacent mucosa from 16 patients who underwent colonoscopy examination. In our study, histologically similar colorectal adenomas showed wide variability in chromosomal instability. Based on the obtained results, we further stratified patients into four distinct groups. The first group showed the gain of MALAT1 and TALAM1, long non-coding RNAs (lncRNAs). The second group involved patients with numerous microdeletions. The third group consisted of patients with a disrupted karyotype. The fourth group of patients did not show any CIN in adenomas. Overall, we identified frequent losses in genes, such as TSC2, COL1A1, NOTCH1, MIR4673, and GNAS, and gene gain containing MALAT1 and TALAM1. Since long non-coding RNA MALAT1 is associated with cancer cell metastasis and migration, its gene amplification represents an important event for adenoma development. 相似文献
22.
Martin Pieper Zinaida Kutelova Sergej Aman Jürgen Tomas 《Advanced Powder Technology》2013,24(1):229-234
A discrete population balance approach to model batch grinding is presented and used to simulate baryte grinding in a vibratory disc mill. The grinding kinetics used in the simulation are measured independently with the grinding rate depending on the particle size. A bimodal fragment size distribution of a single breakage event according to the Austin approach is used. The resulting model was solved by numerical integration and the results are discussed concerning accuracy and applicability. The experimental data are compared with the calculated results and show good agreement with the simulations. The breakage distribution is calculated for different particle sizes. This approach avoids the fitting of a large number of parameters to few experimental values, making the model more robust and allowing a wider range of applications, e.g. process optimization. 相似文献
23.
Tomas Diez 《Nexus Network Journal》2012,14(3):457-468
The ??digital fabrication?? revolution being lived today, both in knowledge creation and in technological developments will become more than a simple formal exploration in architecture and design, or a set of tools exclusive to advanced industries. New tools and processes are becoming more accessible to the masses and are being shared all over the world through Internet platforms, with an open source philosophy, both in software and hardware. The collective mind that is being empowered everyday will define the future of production in the life of mankind and its relation with the environment. The role of architects, engineers, designers and many other professionals, will be reshaped and reconfigured to fit into new models of production and creation. These will need to be supported by new manufacturing platforms, knowledge generating and sharing know-how. 相似文献
24.
KeJun Ning Tomas Kulvicius Minija Tamosiunaite Florentin W?rg?tter 《Journal of Intelligent and Robotic Systems》2012,68(2):165-184
This paper presents a novel trajectory generator based on Dynamic Movement Primitives (DMP). The key ideas from the original DMP formalism are extracted, reformulated and extended from a control theoretical viewpoint. This method can generate smooth trajectories, satisfy position- and velocity boundary conditions at start- and endpoint with high precision, and follow accurately geometrical paths as desired. Paths can be complex and processed as a whole, and smooth transitions can be generated automatically. Performance is analyzed for several cases and a comparison with a spline-based trajectory generation method is provided. Results are comparable and, thus, this novel trajectory generating technology appears to be a viable alternative to the existing solutions not only for service robotics but possibly also in industry. 相似文献
25.
Tomas Bayer 《GeoInformatica》2014,18(3):621-669
This article presents a new off-line method for the detection, analysis and estimation of an unknown cartographic projection and its parameters from a map. Several invariants are used to construct the objective function ? that describes the relationship between the 0D, 1D, and 2D entities on the analyzed and reference maps. It is minimized using the Nelder-Mead downhill simplex algorithm. A simplified and computationally cheaper version of the objective function ? involving only 0D elements is also presented. The following parameters are estimated: a map projection type, a map projection aspect given by the meta pole K coordinates [φ k , λ k ], a true parallel latitude φ 0, central meridian longitude λ 0, a map scale, and a map rotation. Before the analysis, incorrectly drawn elements on the map can be detected and removed using the IRLS. Also introduced is a new method for computing the L 2 distance between the turning functions Θ1, Θ2 of the corresponding faces using dynamic programming. Our approach may be used to improve early map georeferencing; it can also be utilized in studies of national cartographic heritage or land use applications. The results are presented both for the real cartographic data, representing early maps from the David Rumsay Map Collection, and for the synthetic tests. 相似文献
26.
Omar Hernández-González Mondher Farza Tomas Ménard Mohammed M'Saad Rogelio Lozano 《International journal of systems science》2019,50(5):1089-1105
This paper proposes a new high gain observer for a class of non-uniformly observable nonlinear systems with coupled structure driven by sampled outputs. The considered class of systems is particularly constituted by several subsystems where each subsystem is associated to a subset of the output variables. The observer design is carried out through two steps. First, a high-gain observer is proposed in the continuous-time output case under the assumption that an adequate persistent excitation condition is satisfied by each subsystem. Then, the proposed observer is redesigned to handle the case of sampled outputs leading thereby to a continuous-discrete time observer. The latter property is achieved thanks to the approach pursued along the convergence analysis. The effectiveness of the proposed observer is emphasised in a realistic simulation framework involving a mathematical model of a quadrotor which is diffeomorphic to the proposed class of considered systems. 相似文献
27.
28.
Arvani Maedeh Keskinen Jari Railanmaa Anna Siljander Sanna Björkqvist Tomas Tuukkanen Sampo Lupo Donald 《Journal of Applied Electrochemistry》2020,50(6):689-697
Journal of Applied Electrochemistry - In this paper, additive layer-by-layer fabrication of a fully screen printed monolithic supercapacitor exhibiting performance comparable with supercapacitors... 相似文献
29.
Siobhan M. O'Sullivan Tomas Lafarga Maria Hayes Nora M. O'Brien 《International Journal of Food Science & Technology》2017,52(4):1049-1056
Four proteins, α/β globulin, serum albumin, γ‐globulin and fibrinogen, were isolated from bovine blood and hydrolysed using papain. Hydrolysates were assessed for non‐cellular and cellular antioxidant activity. The anti‐proliferative activity of hydrolysed fractions was assessed in a number of cancer cell lines including U937 lymphoma cells, MCF‐7 breast cancer cells, HepG2 hepatocytes and Caco‐2 epithelial colorectal adenocarcinoma cells. Anti‐inflammatory activity of the hydrolysates was also assessed. Hydrolysates generated from γ‐globulin or fibrinogen had significant antioxidant activity in non‐cellular assays. Hydrolysates were also found to be highly toxic to different cancer cell lines, in particular U937 lymphoma cells when assessed using the MTT assay. The fibrinogen hydrolysate was the most toxic sample and toxicity appeared to correlate with its non‐cellular antioxidant activity. None of the hydrolysates had significant anti‐inflammatory activity. The high cytotoxicity of the γ‐globulin and the fibrinogen hydrolysates towards cancer cells may indicate a potential use as anti‐proliferative agents. 相似文献
30.
The kinetics of a batch solid–liquid extraction of total phenolic compounds (PC) from milled grape seed (Vitis vinifera L. cv. “Frankovka”) using 50% ethanol at different extraction temperatures (25–80 °C) was studied. The maximum yield of PC was 0.13 kgGAE/kgdb after 200 min of extraction in agitated vessel at 80 °C. A new model based on the assumptions of a first order kinetics mechanism for the solid–liquid extraction and a linear equilibrium at the solid–liquid interface was developed. The model involves the concept of broken and intact cells in order to describe two successive extraction periods: a very fast surface washing process followed by slow diffusion of phenolic compounds from grape seeds to the solvent.The proposed model is suited to fit experimental data and to simulate the extraction of phenolic compounds, which was confirmed by the correlation coefficient (r ? 0.965), the root mean square error (RMSE ? 0.003 kgGAE/kgdb) and the mean relative deviation modulus (E ? 2.149%). The temperature influenced both equilibrium partition coefficients of phenolic compounds and transport properties, which is manifested by a relatively high value of activation energy (23–24) kJ/mol and by values of effective diffusivity in seed particles. 相似文献