全文获取类型
收费全文 | 367篇 |
免费 | 10篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 15篇 |
综合类 | 1篇 |
化学工业 | 107篇 |
金属工艺 | 3篇 |
机械仪表 | 4篇 |
建筑科学 | 10篇 |
能源动力 | 30篇 |
轻工业 | 49篇 |
水利工程 | 1篇 |
石油天然气 | 14篇 |
无线电 | 17篇 |
一般工业技术 | 61篇 |
冶金工业 | 18篇 |
原子能技术 | 16篇 |
自动化技术 | 33篇 |
出版年
2023年 | 3篇 |
2022年 | 10篇 |
2021年 | 7篇 |
2020年 | 4篇 |
2019年 | 7篇 |
2018年 | 8篇 |
2017年 | 5篇 |
2016年 | 26篇 |
2015年 | 8篇 |
2014年 | 15篇 |
2013年 | 24篇 |
2012年 | 26篇 |
2011年 | 42篇 |
2010年 | 29篇 |
2009年 | 12篇 |
2008年 | 21篇 |
2007年 | 17篇 |
2006年 | 19篇 |
2005年 | 15篇 |
2004年 | 11篇 |
2003年 | 12篇 |
2002年 | 7篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 10篇 |
1997年 | 10篇 |
1996年 | 3篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有379条查询结果,搜索用时 15 毫秒
31.
Liposome‐Based in Vitro Evolution of Aminoacyl‐tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation
下载免费PDF全文
![点击此处可从《Chembiochem : a European journal of chemical biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Dr. Atsuko Uyeda Dr. Takayoshi Watanabe Dr. Yasuhiko Kato Prof. Hajime Watanabe Prof. Tetsuya Yomo Prof. Takahiro Hohsaka Dr. Tomoaki Matsuura 《Chembiochem : a European journal of chemical biology》2015,16(12):1797-1802
Methanosarcina species pyrrolysyl‐tRNA synthetase (PylRS) attaches Pyl to its cognate amber suppressor tRNA. The introduction of two mutations (Y384F and Y306A) into PylRS was previously shown to generate a mutant, designated LysZ‐RS, that was able to attach N‐benzyloxycarbonyl‐L ‐lysine (LysZ) to its cognate tRNA. Despite the potential of LysZ derivatives, further LysZ‐RS engineering has not been performed; consequently, we aimed to generate LysZ‐RS mutants with improved LysZ incorporation activity through in vitro directed evolution. Using a liposome‐based in vitro compartmentalization (IVC) approach, we screened a randomly mutagenized gene library of LysZ‐RS and obtained a mutant that showed increased LysZ incorporation activity both in vitro and in vivo. The ease and high flexibility of liposome‐based IVC should enable the evolution of not only LysZ‐RS that can attach various LysZ derivatives but also of other enzymes involved in protein translation. 相似文献
32.
Proponents of free trade have often hailed international trade as an engine of economic growth. However, the foreign trade sector, like many other sectors in developing countries, frequently involves these countries walking a tightrope between their developmental objectives and environmental goals. In this regard, prioritizing for developing a ‘green’ yet internationally competitive export portfolio provides a quintessential win–win solution to the problem. This study factors in both environmental benignity (indicated by total CO2 emission intensity) as well as trade competitiveness (indicated by revealed comparative advantage index) in identifying the ‘ideal’ Indian export portfolio. The analysis calculates the level of direct and indirect emissions from the foreign trade sector (exports and imports) using the environmental input–output (EIO) matrix for 2003/04 for India that has been jointly developed by researchers from Keio University, Japan, and The Energy and Resources Institute (TERI), New Delhi. The derived basket is compared to the current portfolio to estimate the potential saving from compositional changes and to suggest directions for policymaking to emphasize or de-emphasize the export of certain categories of exports. 相似文献
33.
Masatake Yamaguchi Ken-Ichi Ebihara Mitsuhiro Itakura Tomoko Kadoyoshi Tomoaki Suzudo Hideo Kaburaki 《Metallurgical and Materials Transactions A》2011,42(2):330-339
The microscopic mechanism of grain boundary (GB) embrittlement in metals by hydrogen segregation (trapping) has been not well
understood for many years. From first-principles calculations, we show here that the calculated cohesive energy of bcc Fe
Σ3(111) and fcc Al(Cu) Σ5(012) symmetrical tilt GBs can be significantly reduced if many hydrogen atoms segregate at the GBs.
This indicates that the reduction of the cohesive energy of the GB may cause the hydrogen-induced GB embrittlement in Fe,
Al, and Cu. Considering the “mobile” effect of hydrogen during fracture, especially for the Fe system, more hydrogen atoms
coming from solid solution state can segregate on the gradually formed two fracture surfaces and reduce further the cohesive
energy. We suggest a new idea about the upper and lower critical stresses observed in the constant-load test of hydrogen-induced
delayed fracture in high-strength steels; the upper critical stress is determined by the amount (density) of “immobile” hydrogen
atoms segregated at the GB before fracture, and the lower critical stress is determined by the total amount (density) of immobile
and mobile hydrogen atoms, the latter of which segregate on the two fracture surfaces during fracture. 相似文献
34.
Guisheng Zhai Shohei Okuno Joe Imae Tomoaki Kobayashi 《Journal of Intelligent and Robotic Systems》2011,63(2):309-322
In this paper, we study a consensus problem for multi-agent systems via dynamic output feedback control. The entire system
is decentralized in the sense that each agent can only obtain output information from its neighbor agents. For practical purpose,
we assume that actuator limitation exists, and require that the consensus be achieved among the agents at a specified convergence
rate. By using an appropriate coordinate transformation, we reduce the consensus problem on hand to solving a strict matrix
inequality, and then propose to use the homotopy based method for solving the matrix inequality. It turns out that our algorithm
includes the existing graph Laplacian based algorithm as a special case. 相似文献
35.
Recovery of liquid fuel from hydrocarbon-rich microalgae by thermochemical liquefaction 总被引:11,自引:0,他引:11
Yutaka Dote Shigeki Sawayama Seiichi Inoue Tomoaki Minowa Shin-ya Yokoyama 《Fuel》1994,73(12):1855-1857
Liquefaction of Botryococcus braunii, a colony-forming microalga, with high moisture content was performed with or without sodium carbonate as a catalyst for conversion into liquid fuel and recovery of hydrocarbons. A greater amount of oil than the content of hydrocarbons in B. braunii (50 wt% db) was obtained, in a yield of 57–64 wt% at 300 °C. The oil was equivalent in quality to petroleum oil. The recovery of hydrocarbons was a maximum (>95%) at 300 °C. 相似文献
36.
Kazuki Shibanuma Tomoaki Utsunomiya Shuji Aihara 《International journal for numerical methods in engineering》2014,97(8):551-581
The application of the XFEM to fracture mechanics is effective, because a crack can be modeled independently from the meshes and a complex remeshing procedure can be avoided. However, the classical XFEM has an essential problem in the approximation of partially enriched elements, that is, blending elements, which causes a lack of accuracy. For the weighted XFEM, although the numerical results show the effective improvements, it was found that the issue of blending elements still remains upon detailed examination. In the present paper, the PU‐XFEM is formulated as an explicit application of the partition of unity (PU) approach to the XFEM, in order to precisely reproduce a priori knowledge of the solution by enrichment. The PU‐XFEM is applied to two‐dimensional linear fracture mechanics, and its effectiveness is verified. It is consequently found out that the PU‐XFEM precisely reproduces a priori knowledge of the solution and is therefore effective to completely solve the problem of the blending elements. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
37.
The recent deregulation of the market for electric power in many parts of the US and Canada has expanded the set of potential tools for managing the types of risks faced by both generators and consumers of electric power. In particular manufacturing and other firms whose operations are powered by electricity now face, on a continuing basis, the engineering management decisions concerning whether they should buy or produce electricity, and if they are to buy or sell electricity, what types of contracts are optimum. These types of risk management decisions typically involve futures, forwards, options and other financial derivatives. The price and volatility of electric power are known to play an essential role in determining which of these instruments should be used. However, electricity as a commodity possesses certain special features not shared by other commodities and hence its risk properties are not yet well understood. In this paper we consider and test certain hypotheses about the properties of electricity price using recent market data. We find that electricity prices possess certain volatility and other systematic properties that can be characterized by the type and method of delivery of electricity. These properties can be used by firms in formulating their optimal demand and supply schedules of electric power. 相似文献
38.
Mashimo T Toyama S 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2011,58(4):844-848
Cubic design of a stator in a rotary-linear piezoelectric actuator is sophisticated and interesting, but the vibration theory of the cubic stator remains unclear when using the finite element method (FEM). In this paper, we analyze the vibration behavior of the cubic stator by applying the energy method, which distinguishes the component of mechanical energy. By changing the design of the stator (especially the length in the direction of the through-hole axis), we clarify how the vibration modes are in accordance at one equal frequency in cubic shape. The behavior of the vibration modes is discussed using conventional vibration theory of a beam and a plate. 相似文献
39.
This paper reports on a new technique of measurements of microbubble position in three dimensions with high time-resolution. The technique is based on micro digital holographic particle tracking velocimetry. In this technique, an intensity profile is constructed from a holographic image of a microbubble where the profile results in showing two peaks. The distance between the two peaks appears to relate to the size of the microbubble's diameter. The three-dimensional position of the bubble can be detected by the center of the two peaks and the center point of the bubble image focused by a digital hologram. We also theoretically obtained the intensity profile of a microbubble by considering a refraction of light on a bubble surface to a ring-shaped aperture model. The theoretically obtained distance between the two peaks is found to be in good agreement with the values obtained experimentally. 相似文献
40.
Tomoaki Iwami Kiyoharu Tadanaga Masahiro Tatsumisago Tsutomu Minami Noboru Tohge 《Journal of the American Ceramic Society》1995,78(6):1668-1672
CdS-doped SiO2 glasses were prepared via silica gels containing diethyldithiocarbamatocadmium Cd(S2 CN(C2 H5 )2 )2 . Heat treatment of the gels gave transparent yellow SiO2 glasses doped with hexagonal CdS crystals. In optical absorption and fluorescence spectra, the optical absorption edge and the emission peak clearly exhibited a blue shift, which was attributed to the quantum size effect of the carrier confinement, as the CdS content was decreased. In the fluorescence spectra of the CdS-doped silica glasses, the emission peak was observed only near 500 nm and not observed at longer wavelengths which were known to be present if there were sulfur vacancies. 相似文献