Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta

Spina bifida aperta are complex congenital malformations resulting from failure of fusion in the spinal neural tube during embryogenesis. Despite surgical repair of the defect, most patients who survive with spina bifida aperta have a multiple system handicap due to neuron deficiency of the defective spinal cord. Tissue engineering has emerged as a novel treatment for replacement of lost tissue. This study evaluated the prenatal surgical approach of transplanting a chitosan–gelatin scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in the healing the defective spinal cord of rat fetuses with retinoic acid induced spina bifida aperta. Scaffold characterisation revealed the porous structure, organic and amorphous content. This biomaterial promoted the adhesion, spreading and in vitro viability of the BMSCs. After transplantation of the scaffold combined with BMSCs, the defective region of spinal cord in rat fetuses with spina bifida aperta at E20 decreased obviously under stereomicroscopy, and the skin defect almost closed in many fetuses. The transplanted BMSCs in chitosan–gelatin scaffold survived, grew and expressed markers of neural stem cells and neurons in the defective spinal cord. In addition, the biomaterial presented high biocompatibility and slow biodegradation in vivo. In conclusion, prenatal transplantation of the scaffold combined with BMSCs could treat spinal cord defect in fetuses with spina bifida aperta by the regeneration of neurons and repairmen of defective region.     

马钢2500m~3高炉炉渣的冶金性能

在目前的原燃料条件下,为保证马钢2500m3高炉炉渣具有良好的流动性和较高的脱硫能力,炉渣的CaO／SiO2应控制在1．05至1．15之间,炉渣温度不低于1450℃．     

Tissue dynamics spectroscopy for three-dimensional tissue-based drug screening

Tissue dynamics spectroscopy combines dynamic light scattering with short-coherence digital holography to capture intracellular motion inside multicellular tumor spheroid tissue models. The cellular mechanical activity becomes an endogenous imaging contrast agent for motility contrast imaging. Fluctuation spectroscopy is performed on dynamic speckle from the proliferating shell and hypoxic core to generate drug-response spectrograms that are frequency versus time representations of the changes in spectral content induced by an applied compound or an environmental perturbation. A combination of 28 reference compounds and conditions applied to rat osteogenic UMR-106 spheroids generated spectrograms that were crosscorrelated in a similarity matrix used for unsupervised hierarchical clustering of similar compound responses. This work establishes the feasibility of tissue dynamics spectroscopy for three-dimensional tissue-based phenotypic profiling of drug response as a fully endogenous probe of the response of tissue to reference compounds.     

单磁悬浮系统的纵向解耦控制研究

磁悬浮系统是复杂的强耦合系统,研究中未充分考虑轨道的弹性形变,会造成磁浮列车和轨道之间产生耦合振动的现象,而考虑这一因素对系统的影响无疑增加了研究的难度;为了便于问题的求解,采用非线性系统理论解耦的方法,对单电磁铁悬浮系统进行纵向解耦控制,并对解耦后的子系统进行极点配置使其满足要求的性能指标;仿真结果表明该方法有效地使复杂问题简单化,并且能够实现系统的全局稳定和良好的动态性能.     

基于PolyTrans的IGES文件简化

CAD软件与基于多边形的3D软件之间数据转换需求不断增加,但是CAD模型复杂度和精细度却越来越高,数据转换的难度变大.为了解决这一难题,结合PolyTrans,分析如何解决一般3D软件难以导入大型的IGES(Initial Graphics Exchange Specifi-cation)文件的问题.从PolyTrans模型简化模块的理论依据着手,研究如何控制多边形的数量、降低文件的复杂性和减少文件的数量,使大型CAD装配模型能顺利地以单个实体形式导入Lightwave、3DS MAX以及Maya类的3D软件.并结合具体科研项目,给出了模型优化前后各指标参数对比情况.     

Piecewise Pseudo-Maximum Likelihood Estimation for Risk Aversion Case in First-Price Sealed-Bid Auction

In the literature, more and more clues show that the bidders indeed tend to risk averse. But as a good structure estimation procedure in first-price sealed-bid auctions, the traditional PPMLE (Piecewise Pseudo-Maximum Likelihood Estimation) approach is only applicable to the risk neutrality case. This study generalizes this approach to the risk aversion case, and obtains a simple estimation procedure for the Pareto family of private costs. In the procedure, the estimation order of distribution parameters of private costs is not important, which is different from the traditional PPMLE method. Though there is no unique estimator for θ ₁ and γ, some guidance is given for real-world applications on the basis of our Monte Carlo simulation experiments. What’s more, this procedure can be reduced further, if one is only interested in estimating the private costs. Extensive simulation experimental results indicate that our approach outperforms or matches at least the traditional one in the case of risk neutrality, and is applicable to the risk aversion case.     

Shrinkage estimation in general linear models

We propose a James-Stein-type shrinkage estimator for the parameter vector in a general linear model when it is suspected that some of the parameters may be restricted to a subspace. The James-Stein estimator is shown to demonstrate asymptotically superior risk performance relative to the conventional least squares estimator under quadratic loss. An extensive simulation study based on a multiple linear regression model and a logistic regression model further demonstrates the improved performance of this James-Stein estimator in finite samples. The application of this new estimator is illustrated using Ontario newborn infants data spanning four fiscal years.     

Agent long-term coalition credit

Agent trust researches become more and more important because they will ensure good interactions among the software agents in large-scale open systems. Moreover, individual agents often interact with long-term coalitions such as some E-commerce web sites. So the agents should choose a coalition based on utility and trust. Unfortunately, few studies have been done on agent coalition credit and there is a need to do it in detail. To this end, a long-term coalition credit model (LCCM) is presented. Furthermore, the relationship between coalition credit and coalition payoff is also attended. LCCM consists of internal trust based on agent direct interactions and external reputation based on agent direct observation. Generalization of LCCM can be demonstrated through experiments applied in both cooperative and competitive domain environment. Experimental results show that LCCM is capable of coalition credit computation efficiently and can properly reflect various factors effect on coalition credit. Another important advantage that is a useful and basic property of credit is that LCCM can effectively filter inaccurate or lying information among interactions.     

Evaluation of Landsat 8 OLI imagery for unsupervised inland water extraction

The successful launch of the Landsat 8 satellite continues the Earth observation of the Landsat series, which has been taking place for nearly 40 years. With the increase in the band number and the improved spectral range compared with the previous Landsat imagery, it will be possible to expand the application of the new Landsat 8 imagery. The purpose of this study is to explore water extraction based on the new Landsat 8 Operational Land Imager (OLI) imagery. According to the specific inland water conditions (clear water, turbid water, and eutrophic water), a number of highly adaptable water indices are assessed for water extraction using Landsat OLI imagery. The results show that clear water is the easiest to extract among the different types of waterbodies, with the highest average accuracy of 97%. The highest-accuracy methods are the automated water extraction index for shadow pixels (AWEI_sh), the normalized difference water index using bands 4 and 7 (NDWI₄₇), and the normalized difference water index using bands 3 and 7 (NDWI₃₇), with accuracies of 98.55%, 95.50%, and 96.61%, corresponding to clear water, turbid water, and eutrophic water, respectively. Through the analysis of the different methods for optimal band selection, the seventh band OLI7 (shortwave infrared 2, SWIR-2) of Landsat OLI shows the best performance in water identification. When applying the water indices to water extraction, Otsu’s algorithm has been used to automatically select the water threshold. Using extensive experiments with Otsu’s algorithm and a manual method, it was found that Otsu’s algorithm can replace manual selection and has the ability to select an accurate threshold for water extraction.     

Prediction of rank deficiency in partition of unity-based methods with plane triangular or quadrilateral meshes

In the partition of unity (PU)-based methods, the global approximation is built by multiplying a partition of unity by local approximations. Within this framework, high-order approximations are achieved by directly adopting high-order polynomials as local approximations, and therefore nodes along sides or inside elements, which are usually adopted in the conventional finite element methods, are no more required. However, the PU-based approximation constructed in this way may suffer from rank deficiency due to the linear dependence of the global degrees of freedom. In this paper, the origin of the rank deficiency in the PU-based approximation space is first dissected at an element level, and then an approach to predict the rank deficiency for a mesh is proposed together with the principle of the increase of rank deficiency. Finally, examples are investigated to validate the present approach. The current work indicates such a fact that the rank deficiency is an unrelated issue to the nullity of the global matrix. It can be resolved in its own manner.