Determination of volterra transfer functions of non-linear multiport networks

A method is presented to determine frequency domain Volterra transfer functions of non-linear multiport networks containing non-linear multiport subsystems (elements). the method is based on an extension of the probing method to allow arbitrary (also commensurate) frequencies. A recursively based algorithm for the determination of Volterra transfer functions of non-linear multiport networks is derived. A computer implementation of the method in a symbolic programming language is presented. This makes it possible to determine algebraic expressions of the Volterra transfer functions. Three examples are considered.     

Quantitative liver ADC measurements using diffusion-weighted MRI at 3 Tesla: evaluation of reproducibility and perfusion dependence using different techniques for respiratory compensation

Object

Diffusion weighted imaging (DWI) of the liver suffers from low signal to noise making 3 Tesla (3 T) an attractive option, but 3 T data is scarce. It was the aim to study the influence of different b values and respiratory compensation methods (RCM) on the apparent diffusion coefficient (ADC) level and on ADC reproducibility at 3 T.

Materials and methods

Ten healthy volunteers and 12 patients with malignant liver lesions underwent repeated (2–22 days) breathhold, free-breathing and respiratory triggered DWI at 3 T using b values between 0 and 1,000 s/mm².

Results

The ADCs changed up to 150 % in healthy livers and up to 48 % in malignant lesions depending on b value combinations. Best ADC reproducibility in healthy livers were obtained with respiratory triggering (95 % limits of agreement: ±0.12) and free-breathing (±0.14). In malignant lesions equivalent reproducibility was obtained with less RCM dependence. The use of a lower maximum b value (b = 500) decreased reproducibility (±0.14 to ±0.32) in both normal liver and malignant lesions.

Conclusion

Large differences in absolute ADC values and reproducibility caused by varying combinations of clinically realistic b values were demonstrated. Different RCMs caused smaller differences. Lowering maximum b value to 500 increased limits of agreement up to a factor of two. Serial ADC changes larger than approximately 15 % can be detected confidently on an individual basis in both malignant lesions and normal liver parenchyma at 3 T using appropriate b values and respiratory compensation.     

New protection issues of a matrix converter: design considerationsfor adjustable-speed drives

Induction motors are traditionally controlled by standard pulsewidth modulation voltage-source inverters. An alternative is the matrix converter, which consists of nine bidirectional switches. This converter has benefits compared to a standard inverter, like sinusoidal input current and bidirectional power flow. The main disadvantage is the lack of a bidirectional switch, because such a switch may be done by two transistors and two diodes. An important topic is protection of the matrix converter, and this paper proposes two new protection circuits for matrix converters with a reduced number of components. The number of protection diodes is reduced by six. Design expressions of the protection circuit are calculated and validated by simulation. The standard protection circuit and the new circuits are demonstrated by simulation to have the same performance. Experimental results on an 8 kVA matrix converter show the design expressions are correct. It is concluded that it is possible to reduce the necessary power components in a matrix converter     

Photochemical Mechanism of an Atypical Algal Phytochrome

Phytochromes are bilin‐containing photoreceptors that are typically sensitive to the red/far‐red region of the visible spectrum. Recently, phytochromes from certain eukaryotic algae have become attractive targets for optogenetic applications because of their unique ability to respond to multiple wavelengths of light. Herein, a combination of time‐resolved spectroscopy and structural approaches across picosecond to second timescales have been used to map photochemical mechanisms and structural changes in this atypical group of phytochromes. The photochemistry of an orange/far‐red light‐sensitive algal phytochrome from Dolihomastix tenuilepis has been investigated by using a combination of visible, IR and X‐ray scattering probes. The entire photocycle, correlated with accompanying structural changes in the cofactor/protein, are reported. This study identifies a complex photocycle for this atypical phytochrome. It also highlights a need to combine outcomes from a range of biophysical approaches to unravel complex photochemical and macromolecular processes in multi‐domain photoreceptor proteins that are the basis of biological light‐mediated signalling.     

Raft crystals of poly(isoprene)-block-poly(ferrocenyldimethylsilane) and their surface wetting behavior during melting as observed by AFM and NanoTA

We report on the morphology evolution during heating and melting of lamellar poly(isoprene)-block-poly(ferrocenyldimethylsilane) (PI₇₆-b-PFDMS₇₆) raft crystals deposited at the native oxide surface of silicon (SiO₂) or at a highly ordered pyrolytic graphite (HOPG) surface, studied by in situ temperature controlled atomic force microscopy. Crystals deposited on hydrophilic SiO₂ surfaces revealed an irreversible decrease in length at temperatures of up to tens of degrees above their expected melting temperature, while maintaining their platelet-like structure. Crystals deposited on hydrophobic HOPG surfaces initially decreased in length below their expected melting temperature, while at 120 °C and above a typical molten morphology was observed. In addition, the irreversible formation of a PI₇₆-b-PFDMS₇₆ wetting layer around the crystals was observed upon increasing the temperature. These observations in the morphological behavior upon heating emphasize the role of interfacial energy between a surface deposited block copolymer based macromolecular nanostructure and its supporting substrate.     

Bioavailability Studies and in vitro Profiling of the Selective Excitatory Amino Acid Transporter Subtype 1 (EAAT1) Inhibitor UCPH‐102

Although the selective excitatory amino acid transporter subtype 1 (EAAT1) inhibitor UCPH‐101 has become a standard pharmacological tool compound for in vitro and ex vivo studies in the EAAT research field, its inability to penetrate the blood–brain barrier makes it unsuitable for in vivo studies. In the present study, per os (p.o.) administration (40 mg kg^?1) of the closely related analogue UCPH‐102 in rats yielded respective plasma and brain concentrations of 10.5 and 6.67 μm after 1 h. Three analogue series were designed and synthesized to improve the bioavailability profile of UCPH‐102, but none displayed substantially improved properties in this respect. In vitro profiling of UCPH‐102 (10 μm ) at 51 central nervous system targets in radioligand binding assays strongly suggests that the compound is completely selective for EAAT1. Finally, in a rodent locomotor model, p.o. administration of UCPH‐102 (20 mg kg^?1) did not induce acute effects or any visible changes in behavior.     

Specifying OLAP Cubes on XML Data

On-Line Analytical Processing (OLAP) enables analysts to gain insight about data through fast and interactive access to a variety of possible views on information, organized in a dimensional model. The demand for data integration is rapidly becoming larger as more and more information sources appear in modern enterprises. In the data warehousing approach, selected information is extracted in advance and stored in a repository, yielding good query performance. However, in many situations a logical (rather than physical) integration of data is preferable. Previous web-based data integration efforts have focused almost exclusively on the logical level of data models, creating a need for techniques focused on the conceptual level. Also, previous integration techniques for web-based data have not addressed the special needs of OLAP tools such as handling dimensions with hierarchies. Extensible Markup Language (XML) is fast becoming the new standard for data representation and exchange on the World Wide Web. The rapid emergence of XML data on the web, e.g., business-to-business (B2B) e-commerce, is making it necessary for OLAP and other data analysis tools to handle XML data as well as traditional data formats.Based on a real-world case study, this paper presents an approach to specification of OLAP DBs based on web data. Unlike previous work, this approach takes special OLAP issues such as dimension hierarchies and correct aggregation of data into account. Also, the approach works on the conceptual level, using Unified Modeling Language (UML) as a basis for so-called UML snowflake diagrams that precisely capture the multidimensional structure of the data. An integration architecture that allows the logical integration of XML and relational data sources for use by OLAP tools is also presented.     

The total quasi-steady-state approximation for complex enzyme reactions

Biochemistry in general and enzyme kinetics in particular have been heavily influenced by the model of biochemical reactions known as Michaelis–Menten kinetics. Assuming that the complex concentration is approximately constant after a short transient phase leads to the usual Michaelis–Menten (MM) approximation (or standard quasi-steady-state approximation (sQSSA)), which is valid when the enzyme concentration is sufficiently small. This condition is usually fulfilled for in vitro experiments, but often breaks down in vivo. The total QSSA (tQSSA), which is valid for a broader range of parameters covering both high and low enzyme concentrations, has been introduced in the last two decades. We extend the tQSSA to more complex reaction schemes, like fully competitive reactions, double phosphorylation, Goldbeter–Koshland switch and we show that for a very large range of parameters our tQSSA provides excellent fitting to the solutions of the full system, better than the sQSSA and the single reaction tQSSA. Finally, we discuss the need for a correct model formulation when doing “reverse engineering”, which aims at finding unknown parameters by fitting the model to experimentally obtained data. We show that the estimated parameters are much closer to the real values when using the tQSSA rather than the sQSSA, which overestimates the parameter values greatly.     

Leon J. Yarrow (1921–1982).

Presents an obituary for Leon J. Yarrow. Yarrow was born on September 30, 1921, in Shenandoah, Pennsylvania. His father died when Leon was very young, and he experienced some of the transitions in attachments and care that may well have influenced his life-long research interest in the importance of early experience for the developing child. At age 15 Leon entered the University of Pennsylvania, where he majored in psychology. In 1951 Leon accepted the first research position that was made available in the old U.S. Children's Bureau. He initiated a longitudinal investigation of adopted children that focused on the consequences of the infant's transition from foster care to the adoptive parents. As his findings became known, social work practice was modified to favor adoption at the earliest ages. Leon spent a major part of his career at the National Institutes of Health (NIH). His associates there were uniformly touched by his personal warmth, his unrelentingly high standards for developmental studies, and the conceptual elegance with which he grasped problems. Leon died July 28, 1982, of a heart attack, the day before he planned to embark on a summer vacation with his family. (PsycINFO Database Record (c) 2010 APA, all rights reserved)