Nitrate Accumulation and Other Components of the Groundwater in Relation to Cropping System in an Aquifer in Southwestern Spain

Inappropriate farming techniques and 4 years of drought (1992–1995) produced conditions for high nitrate concentration in an aquifer located in the Province of Seville (Southwest Spain). The objectives of this study were to study groundwater components and to determine the relationship between the cropping system and nitrate concentrations in groundwater. From October 1994 to May 1996, 16 groundwater samples were obtained from a network of 35 sample points. Groundwater concentrations were analysed by multivariate statistical techniques (factor and cluster analysis). Two factors were found: firstly, agricultural pollution and secondly, salinization (from natural mineralization of groundwater and from anthropogenic effects). High levels of NO_3– were closely associated with intensive cotton (Gossypium herbaceum L.) and potato production (Solanum tuberosum L.). The salinization factor includes electrical conductivity, Cl_–, Na₊, Ca_{2 +}, Mg_{2 +}, SO_{42 –} and HCO_3–. Our study permits the impact of crops on groundwater to be determined. In comparison to the other crops, cotton (Gossypium herbaceum L.) and potato (Solanum tuberosum L.) made the greatest contribution to nitrate pollution and salinization. Use of the groundwater for human consumption in drought periods could represent a public health risk. Alternative farming techniques for these crops such as monitored fractional fertilization and controlled irrigation are recommended.     

Bubble cutting circuits

An inhomogeneous applied field can cause an instability in a strip domain that can be used for the controlled generation of bubble domains. These instabilities have been studied experimentally and theoretically by means of a variational calculation. Good agreement is obtained between wall shape calculations and experimental data. Predictions of cutting current as a function of strip width deviate, however, for widths larger than ∼5.5h due to the limitations of the model and the experimental conditions.     

Lowpass filter with minimum integrated power-loss ratio in the passband

A class of lowpass filters with minimum integrated power-loss ratio in the passband is discussed. Comparisons with the usual Butterworth and Cheby?shev filters are also reported.     

Molecular mimicry accompanying HIV-1 infection: human monoclonal antibodies that bind to gp41 and to astrocytes

Monoclonal antibodies that bound to HIV gp41 and cross-reacted with astrocytes were recovered from the blood of three patients infected with HIV-1. Mapping of the specificity of these monoclonal antibodies, using synthetic gp41 peptides, located their epitope to amino acids 644-663 and established their conformation dependence. Six other human monoclonal anti-HIV antibodies were found to bind to HIV gp41 or gp120 but not to reactive astrocytes in brain tissue. Sharing of linear or conformational protein determinants between disparate viral and host proteins is termed molecular mimicry. The consequences of such mimicry by anti-viral antibodies interacting with astrocytes may play a role in the dementia of AIDS patients since a major function of astrocytes is to maintain the appropriate milieu for neuronal function. The finding of such cross-reactive antibodies adds to the evidence for a possible autoimmune pathogenesis in some of the disease manifestations accompanying HIV infection.     

Sources of enterococci in Idiazábal-type cheese

Enterococci are a ubiquitous group and are natural constituents of the intestinal flora of nearly all animals and humans and can reach high levels in a variety of farmhouse cheeses. The purpose of this study was to determine the origin of the different enterococcal strains present in cheeses at different stages of ripening by typing the enterococci isolated from the raw milk, the cheeses, the cheesemaking environment, and from the faecal matter of the ewes and humans associated with cheese production. The potential presence of vancomycin-resistant enterococci (VRE) at all stages of the process and in the cheeses was also considered. The study was carried out at two separate cheesemaking dairy plants, and samples of the ewes' faeces, the shepherds' and cheesemakers' stools, teat cups, vat, brine, holding tank milk, vat milk, and the cheeses after brining and after 1, 15, and 60 days of ripening were collected. Cheesemaking procedures at the two plants were similar, yet the enterococcal levels and species observed differed at all the sample collection points, though E. faecalis predominated in all the milk and cheese samples. The traceability study performed for the species E. faecalis present at all the sample collection points suggested that the cheesemaker and the cheesemaking equipment were the source of the enterococci in the cheeses, though other possible non-faecal sources remain to be determined. VanC1 and vanC2/C3 enterococcal strains were isolated from the ovine faeces, teat cup, brine, and vat samples at cheesemaking dairy plant A, while only two vanC2/C3 strains were isolated from ovine faeces samples at dairy plant B. No VRE strains were detected in any of the milk or cheese samples.     

Breaking a Couple: Disulfide Reducing Agents

Cysteine is present in a large number of natural and synthetic (bio)molecules. Although the thiol side chain of Cys can be in a free form, in most cases it forms a disulfide bond either with a second Cys (bridge) or with another thiol, as in the case of protecting groups. Efficient reduction of these disulfide bridges is a requirement for many applications of Cys-containing molecules in the fields of chemistry and biochemistry. Here we review reducing methods for disulfide bonds, taking into consideration the solubility of the substrates when selecting the appropriate reducing reagent.     

Regional science: economy and geography in France and French-speaking countries

The objective of this paper is to assess the French-speaking countries contributions to regional science since its creation in the 1950s. France, and other French-speaking countries, very quickly adhered to the approach of the founding fathers of regional science. French-language research developed for several years without maintaining major relations with the main streams that flow through regional science. However, the years 2000 and 2010 saw the emergence of streams of thought that strongly irrigate at the international level. The authors of this paper are part of this movement. Here we trace the origins and development of the French-speaking contribution to regional sciences, while highlighting the originality of the French-language approach. First, the question of academic and institutional contexts is discussed, with the role of the founders and the peculiar place of Journals and public institutions. Further sections analyse the main contributions coming from economics (local systems of production and innovation, innovative milieus and proximity analysis) and geography (regional development at a global scale, and urban systems and complexity). We conclude with cross-disciplinary contributions including intra-urban organization and mobility, territorial governance and territorial development, as well as other approaches to the social sciences, in the contemporary issues of city and territorial governance.