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81.
A millistructured reactor and spectroscopic setup for contactless kinetic measurements in oscillating droplets is presented in this work. The polymerization of acrylic acid serves as a model reaction. Design and construction of the reactor focus on the optical access for Raman measurement, the nearly isothermal behavior, and the preservation of droplets during long‐term reactions with a high increase of viscosity. Another key aspect is the possibility of full automation at a later stage. 相似文献
82.
Abstract: Scanning laser vibrometry is a widely used tool to observe Lamb wave fields for structural health monitoring (SHM) purposes. Lamb waves propagate over long distances in thin‐walled structures and interact with structural inhomogeneities, for example, damages, in spite of wavelengths several times of the damage size. In SHM of sheets and glass‐ or carbon‐fibre‐reinforced plastic plates, this effect is used for determining the position as well as the size of structural faults. With the often employed one‐dimensional vibrometry, a geometrically induced, systematic error occurs when measuring oblique‐angled motion. This error can be, in the specific case of Lamb waves, of a non‐negligible quantity. The nature of this geometrical measurement error in general and concerning Lamb waves in special is discussed analytically for both amplitude and phase data. It is shown that this matter should be taken into account in some applications. 相似文献
83.
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85.
Material properties of an elastic material are characterized by the elastic modulus, which is real-valued and constant. For viscoelastic materials, such as plastics and polymers, the relationship between stress and strain is instead dynamic, and characterized by the complex-valued and frequency-dependent complex modulus. It is in this paper described how system identification techniques can be used to determine the complex modulus using strain data from wave propagation experiments on a test specimen. Modeling, derivation of estimators, and analysis of their numerical and statistical properties are included. Identifiability and experimental design are examined in some detail. Several practical examples are presented using real-world data, and a number of extensions are outlined. 相似文献
86.
We describe the use of surface plasmon- and surface plasmon fieldenhanced fluorescence spectroscopy for the detection of hybridization reactions between surface-attached probe oligonucleotides and complement strands binding from solution. These targets, exhibiting different base mismatches relative to the probe 15-mer sequences, carry a fluorophore at their 5'-end thus allowing for sensitive detection and quantification of association, kon, and dissociation, koff, rate constants, as well as affinity constants, Ka. We demonstrate that by the competitive binding / replacement of single strand binding proteins the mismatch discrimination can be further enhanced. 相似文献
87.
88.
Wolfram Witte Daniel Abou‐Ras Karsten Albe Gottfried H. Bauer Frank Bertram Christian Boit Rudolf Brüggemann Jürgen Christen Jens Dietrich Axel Eicke Dimitrios Hariskos Matthias Maiberg Roland Mainz Max Meessen Mathias Müller Oliver Neumann Thomas Orgis Stefan Paetel Johan Pohl Humberto Rodriguez‐Alvarez Roland Scheer Hans‐Werner Schock Thomas Unold Alfons Weber Michael Powalla 《Progress in Photovoltaics: Research and Applications》2015,23(6):717-733
The gallium gradient in Cu(In,Ga)Se2 (CIGS) layers, which forms during the two industrially relevant deposition routes, the sequential and co‐evaporation processes, plays a key role in the device performance of CIGS thin‐film modules. In this contribution, we present a comprehensive study on the formation, nature, and consequences of gallium gradients in CIGS solar cells. The formation of gallium gradients is analyzed in real time during a rapid selenization process by in situ X‐ray measurements. In addition, the gallium grading of a CIGS layer grown with an in‐line co‐evaporation process is analyzed by means of depth profiling with mass spectrometry. This gallium gradient of a real solar cell served as input data for device simulations. Depth‐dependent occurrence of lateral inhomogeneities on the µm scale in CIGS deposited by the co‐evaporation process was investigated by highly spatially resolved luminescence measurements on etched CIGS samples, which revealed a dependence of the optical bandgap, the quasi‐Fermi level splitting, transition levels, and the vertical gallium gradient. Transmission electron microscopy analyses of CIGS cross‐sections point to a difference in gallium content in the near surface region of neighboring grains. Migration barriers for a copper‐vacancy‐mediated indium and gallium diffusion in CuInSe2 and CuGaSe2 were calculated using density functional theory. The migration barrier for the InCu antisite in CuGaSe2 is significantly lower compared with the GaCu antisite in CuInSe2, which is in accordance with the experimentally observed Ga gradients in CIGS layers grown by co‐evaporation and selenization processes. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
89.
Hannelore T. Koller Torsten Klade Adolf Ellinger Michael Breitenbach 《Yeast (Chichester, England)》1996,12(1):53-65
We isolated the Saccharomyces cerevisiae GRC5 (gr owth control) gene by functional complementation in vivo of a ts (t emperature s ensitive) mutation. Phenotypic analysis suggested involvement of GRC5 in cell growth and proliferation. Mutant cells arrest their cell cycles after one to three cell divisions predominantly as mother cells with a large bud. In the region of the septum, a massive accumulation of cell wall material is observed. The mother and daughter nuclei are well separated and spindles are no longer present, while the cytoskeleton is of aberrant appearance. Arrested cells do not perform protein synthesis and are unable to mate. Furthermore, grc5-1ts cells rapidly lose viability at the restrictive temperature (37°C) only on full media, but not under nitrogen-starvation conditions, indicating that proper response to this nutrient limitation is still intact in mutant cells after cell cycle arrest. The sequence of GRC5 translates into a basic protein of 221 amino acids with a corresponding Mr of 25·4 kDa. GRC5 is a member of the highly conserved QM gene family, members of which have been reported from plants, invertebrates and vertebrates. The amino acid sequence of GRC5 over its entire length is more than 60% identical to the human QM protein, expression of which is associated with loss of the tumorigenic phenotype in a cell line derived from Wilms' tumor, a malignancy of the embyronic kidney. Here, we show that GRC5 is an essential yeast gene, the function of which as inferred from analysis of the grc5-1ts mutant is crucial for establishment of proper cytoskeletal structure and regulation of growth in yeast cells. 相似文献
90.
Zamzam Awida Almog Bachar Hussam Saed Anton Gorodov Nathalie Ben-Califa Maria Ibrahim Albert Kolomansky Jennifer Ana Iden Liad Graniewitz Visacovsky Tamar Liron Sahar Hiram-Bab Michael Brines Yankel Gabet Drorit Neumann 《International journal of molecular sciences》2022,23(1)
The two erythropoietin (EPO) receptor forms mediate different cellular responses to erythropoietin. While hematopoiesis is mediated via the homodimeric EPO receptor (EPOR), tissue protection is conferred via a heteromer composed of EPOR and CD131. In the skeletal system, EPO stimulates osteoclast precursors and induces bone loss. However, the underlying molecular mechanisms are still elusive. Here, we evaluated the role of the heteromeric complex in bone metabolism in vivo and in vitro by using Cibinetide (CIB), a non-erythropoietic EPO analogue that exclusively binds the heteromeric receptor. CIB is administered either alone or in combination with EPO. One month of CIB treatment significantly increased the cortical (~5.8%) and trabecular (~5.2%) bone mineral density in C57BL/6J WT female mice. Similarly, administration of CIB for five consecutive days to female mice that concurrently received EPO on days one and four, reduced the number of osteoclast progenitors, defined by flow cytometry as Lin−CD11b−Ly6Chi CD115+, by 42.8% compared to treatment with EPO alone. In addition, CIB alone or in combination with EPO inhibited osteoclastogenesis in vitro. Our findings introduce CIB either as a stand-alone treatment, or in combination with EPO, as an appealing candidate for the treatment of the bone loss that accompanies EPO treatment. 相似文献