Mechanical property enhancement of aligned multi-walled carbon nanotube sheets and composites through press-drawing process

A solid-state drawing and winding process was done to create thin aligned carbon nanotube (CNT) sheets from CNT arrays. However, waviness and poor packing of CNTs in the sheets are two main weaknesses restricting their reinforcing efficiency in composites. This report proposes a simple press-drawing technique to reduce wavy CNTs and to enhance dense packing of CNTs in the sheets. Non-pressed and pressed CNT/epoxy composites were developed using prepreg processing with a vacuum-assisted system. Effects of pressing on the mechanical properties of the aligned CNT sheets and CNT/epoxy composites were examined. Pressing with distributed loads of 147, 221, and 294 N/m showed a substantial increase in the tensile strength and the elastic modulus of the aligned CNT sheets and their composites. The CNT sheets under a press load of 221 N/m exhibited the best mechanical properties found in this study. With a press load of 221 N/m, the pressed CNT sheet and its composite, respectively, enhanced the tensile strength by 139.1 and 141.9%, and the elastic modulus by 489 and 77.6% when compared with non-pressed ones. The pressed CNT/epoxy composites achieved high tensile strength (526.2 MPa) and elastic modulus (100.2 GPa). Results show that press-drawing is an important step to produce superior CNT sheets for development of high-performance CNT composites.     

Reproducible Enhancement of Fluorescence by Bimetal Mediated Surface Plasmon Coupled Emission for Highly Sensitive Quantitative Diagnosis of Double‐Stranded DNA

Plasmonic enhancement of fluorescence from SYBR Green I conjugated with a double‐stranded DNA (dsDNA) amplicon is demonstrated on polymerase chain reaction (PCR) products. Theoretical computation leads to use of the bimetallic (Au 2 nm–Ag 50 nm) surface plasmons due to larger local fields (higher quality factors) than monometallic (Ag or Au) ones at both dye excitation and emission wavelengths simultaneously, optimizing fluorescence enhancement with surface plasmon coupled emission (SPCE). Two kinds of reverse Kretschmann configurations are used, which favor, in signal‐to‐noise ratio, a fluorescence assay that uses optically dense buffer such as blood plasma. The fluorescence enhancement (12.9 fold at maximum) with remarkably high reproducibility (coefficient of variation (CV) < 1%) is experimentally demonstrated. This facilitates credible quantitation of enhanced fluorescence, however unlikely to obtain by localized surface plasmons. The plasmon‐induced optical gain of 46 dB due to SPCE‐active dye molecules is also estimated. The fluorescence enhancement technologies with PCR enables LOD of the dsDNA template concentration of ≈400 fg µL^?1 (CV < 1%), the lowest ever reported in DNA fluorescence assay to date. SPCE also reduces photobleaching significantly. These technologies can be extended for a highly reproducible and sufficiently sensitive fluorescence assay with small volumes of analytes in multiplexed diagnostics.     

Apolipoprotein E*3-Leiden transgenic mice as a test model for hypolipidaemic drugs

PURPOSE: We report an investigation into the distribution of proteoglycans (PGs) in normal, organ-cultured and dextran-treated human corneas. METHODS: Immunogold labeling was carried out at the electron microscope level to localize keratan sulphate (KS), chondroitin sulphate (CS), and heparan sulphate (HS) PGs. RESULTS: High levels of labeling for CS was found in the epithelium, endothelium, and keratocytes, with light labelling present in the basement membranes and the corneal stroma. Labeling for HS was present in the epithelium, endothelium, and keratocytes, with intense labeling present at the endothelium/Descemet's membrane interface and the epithelium/Bowman's layer interface. Large filaments were also observed in these regions in cuprolinic blue-stained specimens. Keratan sulphate was present at high levels in the stroma and the basement membranes with low levels present within the keratocytes, epithelium, and endothelium. The pattern of KS labeling along the collagen fibrils in the stroma sometimes showed evidence of periodicity. Organ-cultured corneas had extensive collagen-free "lakes," the interior of which immunolabeled positively for KS and showed staining with cuprolinic blue. The lakes were greatly reduced in the dextran-treated samples. CONCLUSION: This investigation determined the ultrastructural distribution of KS, CS, and HS PGs in human cornea and showed that organ culture is associated with a change in distribution of stromal PGs.     

Integratable non-clogging microconcentrator based on counter-flow principle for continuous enrichment of CaSki cells sample

This study addresses the need to reduce the risk of clogging when preparing samples for cell concentration, i.e., the CaSki Cell-lines (epidermoid cervical carcinoma cells). Aiming to develop a non-clogging microconcentrator, we proposed a new counter-flow concentration unit characterized by the directions of penetrating flows being at an obtuse angle to the main flow, due to employment of streamlined turbine blade-like micropillars. Based on the optimization results of the counter-flow unit profile, a fractal arrangement for the counter-flow concentration unit was developed. A counter-flow microconcentrator chip was then designed and fabricated, with both the processing layer and collecting layer arranged in terms of the honeycomb structure. Visualized experiments using CaSki cell samples on the microconcentrator chip demonstrated that no cell-clogging phenomena occurred during the test and that no cells were found in the final filtrate. The test results show an excellent concentration performance for the microconcentrator chip, while a concentrating ratio of >4 with the flow rate being below 1.0 ml/min. As only geometrical structure is employed in the passive device, the counter-flow microconcentrator can be easily integrated into advanced microfluidic systems. Owing to the merit of non-clogging and continuous processing ability, the counter-flow microconcentrator is not only suitable for the sample preparation within biomedical field, but also applicable in water-particle separation.     

Combination of convex theories: Modularity,deduction completeness,and explanation

Decision procedures are key components of theorem provers and constraint satisfaction systems. Their modular combination is of prime interest for building efficient systems, but their effective use is often limited by poor interface capabilities, when such procedures only provide a simple “sat/unsat” answer. In this paper, we develop a framework to design cooperation schemas between such procedures while maintaining modularity of their interfaces. First, we use the framework to specify and prove the correctness of classic combination schemas by Nelson–Oppen and Shostak. Second, we introduce the concept of deduction complete satisfiability procedures, we show how to build them for large classes of theories, then we provide a schema to modularly combine them. Third, we consider the problem of modularly constructing explanations for combinations by re-using available proof-producing procedures for the component theories.     

Spin-valve planar Hall sensor for single bead detection

The planar Hall effect (PHE) sensor with a junction size of 3 μm × 3 μm for a single micro-bead detection has been fabricated successfully using a typical spin-valve thin film Ta(5)/NiFe(16)/Cu(1.2)/NiFe(2)/IrMn(15)/Ta(5) nm. The PHE sensor exhibits a sensitivity of about 7.2 μV Oe^?1 in the magnetic field range of ±7 Oe approximately. We have performed an experiment to illustrated the possibility of single micro-bead detection by using a PHE sensor. A single micro-bead of 2.8 μm diameter size is secluded from 0.1% dilute solution of the Dynabeads^® M-280 dropped on the sensor surface and is located on the sensor junction by using a micro magnetic needle. The comparison of the PHE voltage profiles in the field range from 0 to 20 Oe in the absence and presence of a single micro-bead identifies a single Dynabeads^® M-280, the maximal signal change as large as ΔV ～ 1.1 μV can be obtained at the field ～6.6 Oe. The results are well described in terms of the reversal of a basic single domain structure.     

TRUST BY ASSOCIATION: A META‐REPUTATION SYSTEM FOR PEER‐TO‐PEER NETWORKS

Trust mechanisms are used in peer‐to‐peer (P2P) networks to help well‐behaving peers find other well‐behaving peers with which to trade. Unfortunately, these trust mechanisms often do little to keep badly behaving peers from entering and taking advantage of the network, which makes the resulting network difficult or impossible to use for legitimate purposes such as e‐commerce. We propose trust by association, a way of tying peers together in invitation‐only P2P networks in such a way as to encourage the removal of badly behaving peers. We use invitations to create a structure within the otherwise ad hoc P2P network. Using this structure, we create a meta‐reputation system where we measure a peer’s trustworthiness not only by its own behavior, but also by the behavior of the peers it has invited to join. The connection created between the peers takes advantage of the external social relationship that must exist before a peer can be invited into the network. The result is a P2P network where, rather than just trying to marginalize badly behaving peers, there is incentive to kick them out of the network. We present results from a simple simulation showing that our approach works well in general when combined with and compared to an existing trust mechanism.     

Improving traversability of quadruped walking robots using body movement in 3D rough terrains

This paper presents a study on improving the traversability of a quadruped walking robot in 3D rough terrains. The key idea is to exploit body movement of the robot. The position and orientation of the robot are systematically adjusted and the possibility of finding a valid foothold for the next swing is maximized, which makes the robot have more chances to overcome the rough terrains. In addition, a foothold search algorithm that provides the valid foothold while maintaining a high traversability of the robot, is investigated and a gait selection algorithm is developed to help the robot avoid deadlock situations. To explain the algorithms, new concepts such as reachable area, stable area, potential search direction, and complementary kinematic margin are introduced, and the effectiveness of the algorithms is validated via simulations and experiments.     

Integrated micro Pirani gauge based hermetical package monitoring for uncooled VO x bolometer FPAs

This paper presents the design and fabrication of a micro Pirani gauge using VO _x as the sensitive material for monitoring the pressure inside a hermetical package for micro bolometer focal plane arrays (FPAs). The designed Pirani gauge working in heat dissipating mode was intentionally fabricated using standard MEMS processing which is highly compatible with the FPAs fabrication. The functional layer of the micro Pirani gauge is a VO _x thin film designed as a 100 × 200 μm pixel, suspended 2 μm above the substrate. By modeling of rarefied gas heat conduction using the Extended Fourier’s law, finite element analysis is used to investigate the sensitivity of the pressure gauge. Also the thermal interactions between the micro Pirani gauge and bolometer FPAs are verified. From the fabricated prototype, the measured device TCR is about −0.8% K⁻¹ and the sensitivity about 1.84 × 10⁻³ W K⁻¹ mbar⁻¹.