Large-scale protein identification of human urine proteome by multi-dimensional LC and MS/MS

Urine is a human specimen that is easily obtained non-invasively for clinical diagnosis. We attempted to enhance the resolution of current human urine proteomes and construct a comprehensive reference database for advanced studies, such as the discovery of biomarkers for renal diseases. Multi-dimensional LC-MS/MS was coupled with de novo sequencing and database matching. The proposed approach improved the identification of not only the proteins, but also the post-translational sites of urinary proteins. We identified 165, 200 and 259 unique gene products in the urine proteomes from males, females and pregnant women, respectively. When all of the results were combined and the redundancies removed, a total of 1095 distinct peptides were identified. Of these, 1016 peptides were associated with 334 unique gene products. In this study, over 100 gene products, including some disease-related proteins, were detected in urine for the first time by proteomic approaches. Various proteins with novel post-translational hydroxylation were identified using the MASCOT program and de novo sequencing. All proteins with peptide information were summarized into a comprehensive urine protein database. We believe that this comprehensive urine proteome database will assist in the identification of urinary proteins/polypeptides whose spectra are difficult to interpret in the discovery of urinary biomarkers.     

Noise of He-Cd laser and its suppression

Linear relations have been observed between the reciprocal of the noise ratio and the reciprocal of the output power of the He-Cd laser as well as between the noise ratio and the net gain within the laser cavity. By a feedback method with an acoustooptic modulator in the laser cavity at the Brewster angle for low optical loss, a stable laser with high power output and low noise has been obtained. The noise ratio and the output power are 0.8% and 33 mW, respectively, at 4416 A     

Deformation Behavior of Duplex Zircaloy-4-Oxygen Alloys

The mechanical properties of two-phase Zircaloy-4-oxygen alloys at thermal equilibrium have been determined. The strength of these alloys depends to a large extent on their microstructure. The strengthening behavior for alloys having isolateda grains in the softer β matrix is similar to the dispersed particle strengthening. The yield strength of these alloys is found to obey the Petch relationship, (MPa), whereλ _β is the mean free path of β phase inμm. As the volume fraction ofa phase increases, its aspect ratio also increases. This allows more effective load transfer from the matrix to the hardera phase. It has been shown that the strength of these alloys obeys a modified rule of mixtures. The alloys having equiaxeda grains in the β matrix show large strain rate sensitivities at low strain rate. The deformation behavior is interpreted in terms of dislocation slip in the β matrix and diffusion assisted climb near the interphase boundary.     

Polymeric carbon nanocomposites from multiwalled carbon nanotubes functionalized with segmented polyurethane

Multiwalled carbon nanotubes (MWNT) were functionalized with segmented polyurethanes (PU) by the “grafting to” approach. Raman and X‐ray photoelectron spectroscopy (XPS) spectra show that the sidewalls of MWNTs have been functionalized with acid treatment, and the amount of COOH increases with increasing acid treatment time. FTIR and X‐ray diffraction (XRD) spectra confirm that PU is covalently attached to the sidewalls of MWNTs by esterification reaction. Similar to the parent PU, the functionalized carbon nanotube samples are soluble in highly polar solvents, such as dimethyl sulfoxide (DMSO) and N,N‐dimethylformamide (DMF). The functionalized acid amount and the grafted PU amount were determined by thermogravimetric analyses (TGA). Comparative studies, based on SEM images between the PU‐functionalized and chemically defunctionalized MWNT samples, also reveal the covalent coating character. Dynamic mechanical analysis (DMA) of nanocomposite films prepared from PU and PU‐functionalized MWNTs show enhanced mechanical properties and increased soft segment T_g. Tensile properties indicate that PU‐functionalized MWNTs are effective reinforcing fillers for the polyurethane matrix. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007     

Lane Detection With Moving Vehicles in the Traffic Scenes

A lane-detection method aimed at handling moving vehicles in the traffic scenes is proposed in this brief. First, lane marks are extracted based on color information. The extraction of lane-mark colors is designed in a way that is not affected by illumination changes and the proportion of space that vehicles on the road occupy. Next, for vehicles that have the same colors as the lane marks, we utilize size, shape, and motion information to distinguish them from the real lane marks. The mechanism effectively eliminates the influence of passing vehicles when performing lane detection. Finally, pixels in the extracted lane-mark mask are accumulated to find the boundary lines of the lane. The proposed algorithm is able to robustly find the left and right boundary lines of the lane and is not affected by the passing traffic. Experimental results show that the proposed method works well on marked roads in various lighting conditions     

Synthesis of BaTi4O9 ceramics by reaction-sintering process

Synthesis of BaTi₄O₉ ceramics by a reaction-sintering process was investigated. The mixture of raw materials for stoichiometric BaTi₄O₉ were pressed and sintered into ceramics without any calcination stage involved. Pure BaTi₄O₉ phases were obtained at 1150-1280 °C. High-sintered density, 98.2-99.5% of theoretical value (4.533 g/cm³), can be obtained for pellets sintered at 1200-1280 °C for 2-6 h. Some rod-shaped grains 3-7 μm in the longitudinal axis appear in pellets sintered at 1230 °C. Both the size and the amount of these rod-shaped grains increase at higher sintering temperature.     

New initial position detection technique for three-phase brushless DC motor without position and current sensors

This paper presents a new detection technique of initial position for a three-phase brushless DC motor which does not require any current and position sensors, and thereby significantly reduces the cost. The fundamental principle of the technique is to determine the initial position of the permanent magnet by the time periods of discharge of stator windings, which are excited before discharge. As compared to previous approaches, the presented technique does not cause any rotation during detection, and it is therefore very promising for particular kinds of applications, which do not allow the motor to rotate potentially in the wrong direction during initial position detection, e.g., electrical vehicles. As compared to earlier techniques, the presented technique dramatically simplifies the detection procedures and cost. Experimental results derived from a field-programmable gate-array-based control system are presented to demonstrate the feasibility of the presented technique.     

Diverse reasoning in automated model formulation

Diverse reasoning supports a dynamic integration of various reasoning methods in a computerized system. This paper describes a control blackboard approach to simulate the control features observed in the expert's model formulation protocols. The diverse reasoning concept is incorporated so that the model formulation process is dynamically in a plan-directed, action-directed, or data-directed fashion. The diverse reasoning concept facilitates the control features simulation. By analyzing the diverse reasoning behavior in the proposed system, this paper contributes to a better understanding of and support to the modeling process for the design of intelligent decision support systems. The usefulness of the prototype system is also evaluated using an empirical experiment.     

Product family modeling for mass customization

With growing reliance on modeling in product development, it is imperative to describe product families in a cohesive way. In particular, mass customization calls for a close integration of product life cycle from customer recognition to delivery and services. This paper proposes a triple-view scheme for modeling product families. Technical challenges are discussed by comparing product family modeling with modeling single products. Individual modeling formalisms for different views are discussed. An example of product family modeling in power supply design is presented to illustrate the feasibility and potential of the proposed approach.