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101.
C. Kraft M. HädrichH. Metzner U. ReislöhnerP. Schley R. Goldhahn 《Thin solid films》2011,519(21):7173-7175
The excitonic luminescence band of polycrystalline cadmium telluride layers has been investigated by Photoluminescence (PL) and Photoluminescence excitation spectroscopy (PLE). CdTe was deposited by means of close space sublimation and the samples were activated by different chlorine containing compounds, i.e. cadmium chloride, hydrochloric acid, and sodium chloride as well as by simple air activation or received no post deposition treatment. In the PL spectra, four different peaks within the excitonic luminescence band were resolved. These include the free-exciton peak and two transitions of excitons bound to defects. Furthermore, free excitons and band to band transitions were detected by means of PLE. The PL and PLE spectra are discussed with respect to the post deposition treatments. 相似文献
102.
103.
D Dormann C Ebner ER Jarman E Montermann D Kraft AB Reske-Kunz 《Canadian Metallurgical Quarterly》1998,28(11):1374-1383
BACKGROUND: Allergoids are widely used in specific immunotherapy for the treatment of IgE-mediated allergic diseases. OBJECTIVE: The aim of this study was to analyse whether a modification of birch pollen allergens with formaldehyde affects the availability of T-cell epitopes. METHODS: Efficient modification of the allergens was verified by determining IgE and IgG binding activity using ELISA inhibition tests. T-cell responses to birch pollen allergoids were analysed in polyclonal systems, using peripheral blood mononuclear cells (PBMC) of five birch pollen-allergic individuals, as well as birch pollen extract-reactive T-cell lines (TCL), established from the peripheral blood of 14 birch pollen-allergic donors. To determine whether the modification of natural (n)Bet v 1 with formaldehyde or maleic anhydride results in epitope-specific changes in T-cell reactivities, 22 Bet v 1-specific T-cell clones (TCC), established from nine additional birch pollen-allergic individuals, were tested for their reactivity with these products. RESULTS: The majority of PBMC and TCL showed a reduced response to the birch pollen extract allergoid. Bet v 1-specific TCC could be divided into allergoid-reactive and -non-reactive TCC. No simple correlation between possible modification sites of formaldehyde in the respective T-cell epitopes and the stimulatory potential of the allergoid was observed. Mechanisms of suppression or of anergy induction were excluded as an explanation for the non-reactivity of representative TCC. All TCC could be stimulated by maleylated and unmodified nBet v 1 to a similar extent. CONCLUSION: These results demonstrate differences in the availability of T-cell epitopes between allergoids and unmodified allergens, which are most likely due to structural changes within the allergen molecule. 相似文献
104.
Uterine expression of angiogenic factors (vascular endothelial growth factor [VEGF] and basic fibroblast growth factor [bFGF]) was evaluated in ovariectomized ewes at 0, 2, 4, 8, 24, 48, or 72 h after estradiol (E2) treatment. Endometrial VEGF mRNA increased more than 5-fold from 0 to 4 h, remained elevated at 8 h, and then declined through 72 h after E2 treatment. In contrast, endometrial bFGF mRNA remained constant from 0 to 4 h, increased 2.2-fold from 4 to 8 h, remained elevated at 24 h, and then declined through 72 h. Immunostaining for VEGF was present in myometrial and endometrial microvessels (arterioles, venules, and/or capillaries) and also in myometrial smooth muscle; the pattern of VEGF immunostaining followed that of mRNA expression, being elevated at 4 and 8 h after E2 treatment. Immunostaining for bFGF was present exclusively in uterine glands; the pattern of bFGF immunostaining also followed that of its mRNA, being elevated at 8 and 24 h after E2. On the basis of these observations, we suggest that VEGF and bFGF are probably important factors responsible for the dramatic uterine microvascular response that occurs 8 to 24 h after E2 treatment in ovariectomized ewes. 相似文献
105.
EM Perchellet JB Ladesich Y Chen HS Sin DH Hua SL Kraft JP Perchellet 《Canadian Metallurgical Quarterly》1998,9(6):565-576
Methods of both linkage analysis and association analysis may be model-based or model-free. The former are useful for initial exploratory analysis, the latter for more detailed multivariate genometric analysis. Linkage leads to an association, but that association may be solely intrafamilial. Allelic association may be due to pleiotropy, linkage disequilibrium, meiotic drive, selection, or population stratification. Using non-transmitted parental alleles as controls for alleles transmitted to cases, in conjunction with a McNemar-type test, does not detect association in the absence of linkage. Model-based analyses should use models that approximate the complexity of the disease being studied in order to be both robust and powerful. 相似文献
106.
107.
Campbell P.M. Greub H.J. Garg A. Steidl A. Carlough S. Ernest M. Philhower R. Maier C. Kraft R.P. McDonald J.F. 《Very Large Scale Integration (VLSI) Systems, IEEE Transactions on》1998,6(1):52-55
A digital voltage-controlled oscillator (VCO) is described which uses frequency multiplication and division to achieve very wide bandwidth. The VCO uses current-mode logic and does not require reactive elements such as inductors, capacitors or varactors. A novel, fully symmetric exclusive-OR (XOR) circuit was developed which uses product pairs and emitter-coupled logic. To achieve the highest performance possible, the critical path is symmetric and special physical design techniques were developed to promote matched-capacitance. The maximum measured frequency was 13.66 GHz. The chip occupies 1.9 mm×1.6 mm and dissipates 2.45 W at a supply voltage of -6.0 V. With a measured frequency range from 1.25 to 13.66 GHz, this circuit has the widest bandwidth reported in the literature for any VCO, digital or analog 相似文献
108.
An important goal for the design of visual displays is to determine how much realism or scene complexity to include in a computer simulation to reach a given level of performance. This is an important task since the present trend in computer graphics is to include the highest level of realism or scene complexity possible in a simulation. However, it may not be necessary to always include the highest level of realism or complexity to reach an acceptable level of performance. In fact, needless degrees of realism, and thus computational resources, may be wasted in the quest for ‘photographic’ realism. To study the relationship between scene complexity and human performance, three different simulations of scene complexity were modeled for a final approach task. The subject's task were to estimate two aspects of situation awareness, perceived altitude and aimpoint, during a simulated final approach at nine unique distances to threshold. The three levels of scene complexity included a homogeneous Lambertian shaded flat surface, farmlands, and farmlands with hills. The results indicated that increasing the level of scene complexity lead to better performance in judging both altitude and aimpoint during the simulated final approach. The relationship between scene complexity and perceptual performance for computer graphics simulations are discussed. 相似文献
109.
A. Brüning und B. Kraft 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1927,54(4):347-352
Ohne Zusammenfassungbedeutet mit Abbildungen. 相似文献
110.
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor 总被引:4,自引:0,他引:4
U Kutay FR Bischoff S Kostka R Kraft D G?rlich 《Canadian Metallurgical Quarterly》1997,90(6):1061-1071
NLS proteins are transported into the nucleus by the importin alpha/beta heterodimer. Importin alpha binds the NLS, while importin beta mediates translocation through the nuclear pore complex. After translocation, RanGTP, whose predicted concentration is high in the nucleus and low in the cytoplasm, binds importin beta and displaces importin alpha. Importin alpha must then be returned to the cytoplasm, leaving the NLS protein behind. Here, we report that the previously identified CAS protein mediates importin alpha re-export. CAS binds strongly to importin alpha only in the presence of RanGTP, forming an importin alpha/CAS/RanGTP complex. Importin alpha is released from this complex in the cytoplasm by the combined action of RanBP1 and RanGAP1. CAS binds preferentially to NLS-free importin alpha, explaining why import substrates stay in the nucleus. 相似文献