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81.
Pratyush Kumar Mishra Chang-Mo Yoo Dr. Eunmi Hong Prof. Dr. Hyun Woo Rhee 《Chembiochem : a European journal of chemical biology》2020,21(7):924-932
Studying protein–protein interactions (PPIs) is useful for understanding cellular functions and mechanisms. Evaluating these PPIs under conditions as similar as possible to native conditions can be achieved using photo-crosslinking methods because of their on-demand ability to generate reactive species in situ by irradiation with UV light. Various fusion tag, metabolic incorporation, and amber codon suppression approaches using various crosslinkers containing aryl azide, benzophenone, and diazirines have been applied in live cells. Mass spectrometry and immunological techniques are used to identify crosslinked proteins based on their capture transient and context-dependent interactions. Herein we discuss various incorporation methods and crosslinkers that have been used for interactome mapping in live cells. 相似文献
82.
83.
Swati Garg Marlene J. Cran Vijay Kumar Mishra 《International Journal of Food Science & Technology》2019,54(5):1892-1900
The effects of different pH treatments with and without heating on the characteristics of wheat gluten suspension was investigated. At pH 1, maximum changes in colour were observed with a concurrent 65% decrease in protein free-thiol content compared to the control gluten. The SDS-Extractability of protein (SDS-EP) chromatogram eluted at lower retention time and the presence of bands at the top lane even during reducing conditions in SDS-PAGE gel suggested complex formation involving bonds other than disulphides. An increase in the free-amino group content and the presence of an additional peak at a higher retention time in the SDS-EP chromatogram was suggestive of hydrolysis. At pH 2 and 3, similar decreases in SDS-EPs and free-thiol content indicated formation of complexes. When heated, the free-thiol content of the dispersions increased compared to the non-heated dispersions indicating disruption of disulphide bonds with changes in gluten structure and size distribution. 相似文献
84.
Anomaly detection for symbolic sequence data is a highly important area of research and is relevant in many application domains. While several techniques have been proposed within different domains, understanding of their relative strengths and weaknesses is limited. The key factor for this is that the nature of sequence data varies significantly across domains, and hence while a technique might perform well in its original domain, its performance is not guaranteed in a different domain. In this paper, we aim at establishing this understanding for a wide variety of anomaly detection techniques for symbolic sequences. We present a comparative evaluation of a large number of anomaly detection techniques on a variety of publicly available as well as artificially generated data sets. Many of these are existing techniques while some are slight variants and/or adaptations of traditional anomaly detection techniques to sequence data. The analysis presented in this paper allows relative comparison of the different anomaly detection techniques and highlights their strengths and weaknesses. We extend the reference based analysis (RBA) framework, which was originally proposed to analyze multivariate categorical data, to analyze symbolic sequence data sets. We visualize the symbolic sequences using the characteristics provided by the RBA framework and use the visualization to understand various aspects of the sequence data. We then use the characterization done by RBA to understand the performance of the different techniques. Using the RBA framework, we propose two anomaly detection techniques for symbolic sequences, which show consistently superior performance over the existing techniques across the different data sets. 相似文献
85.
Self-sustainable combustion and narrow stability limits are the most critical issues in microcombustors (length scale 1 mm) as increased heat losses can lead to thermal quenching of the flame. Hydrogen is a potential fuel for microcombustion due to its high specific energy and wide flammability limits. This work focuses on the lean premixed hydrogen-air flames stabilized in a newly developed annular microcombustor. Detailed axisymmetric numerical calculations involving multistep kinetics, multicomponent mass-heat transport, conjugate heat transfer, and thermal radiation in gas and solid media are performed. It has been shown that flame stabilization occurs by preheating even though wall temperatures are higher than the autoignition temperature. Results unravel the importance of H radical reactions in the kinetics of microflames in low- and high-temperature regions of the microcombustor. 相似文献
86.
Raman K. Mishra Vasanthakumaran Sudarsan Savita Jain Chetan P. Kaushik Rajesh K. Vatsa Avesh K. Tyagi 《Journal of the American Ceramic Society》2014,97(2):427-431
Barium sodium borosilicate glasses containing different amounts of uranium oxides were prepared by conventional melt quench method and investigated for their structural aspects by 29Si and 11B MAS NMR technique combined with steady‐state luminescence and lifetime measurements. Based on MAS NMR studies, it is confirmed that uranium ions act as network modifier up to 15 wt% and beyond which a separate uranium containing phase is formed. From the luminescence studies, it is inferred that uranyl species is in a highly distorted environment. For more than 15 wt% uranium oxide incorporation, weaker U–O–U linkages are formed at the expense stronger U–O–Si/B linkages, as suggested by the excited state lifetime value of the uranyl species as well as red shift in emission peak maximum. For glass samples containing more than 25 wt% uranium oxides, crystalline barium uranium silicate gets phase separated from glass matrix as confirmed by XRD studies. 相似文献
87.
Balachandra P. Shetty Sudheer Reddy R. K. Mishra 《Journal of Failure Analysis and Prevention》2017,17(4):750-755
The paper describes the methodology of modeling and simulation of bird impact mechanism of GLARE laminate structures. The bird is modeled using Lagrangian concept. Explicit finite element techniques have been developed to simulate the impact mechanics. The study involves deeper understanding of impact dynamics and contact mechanics. The bird impact analysis has been carried out on typical configuration of GLARE 3/2, 4/3, 5/4, 6/5, 7/6 and 8/7. The results of stress propagation and material deformation at high strain rate have been obtained. Results from the numerical analysis are compared with experimental results, and the material is found to be capable of absorbing the impact energy. The results also show that the bird material model chosen to simulate for carrying out impact mechanics analysis is found to be capable of capturing most of the complex behavior exhibited by functional structural material GLARE. 相似文献
88.
89.
Prapti Chaudhari Satyendra Mishra 《Journal of Materials Science: Materials in Electronics》2018,29(1):117-123
The NiTiO3 nanoparticles with ilmenite phase were synthesized by the sol gel method and investigated for sensing of various volatile organic compounds. The resistive type sensing by NiTiO3 towards LPG was not reported earlier where the sensor showed fair response towards hydrogen sulfide gas, but the sensitivity towards LPG was very high. The response % for 150 ppm of LPG was approximately 3200% at room temperature. The high response towards LPG was due to the presence of some amount of rutile TiO2 in the composites. The response and recovery times of these sensors were very less (about 2 s) which could be attributed to the whiskers like structure of NiTiO3. 相似文献
90.
Nowadays, renewable energy systems have come up with more potential in power generation so as to meet the power demand. Among all the renewable systems, the wind energy generating system is believed to be at the peak. However, the wind energy‐based microgrid system is associated with many problems such as fluctuations in output voltage due to the fluctuated wind speeds and harmonics generations in the system. To address these issues, this article proposes a new method in order to achieve harmonic mitigation across its output by maintaining constant voltage. Nevertheless, particular attention has been given to the form and function of modular multilevel converter with multi‐winding transformer connected to the grid. Modular multilevel converter has been implemented with an advanced voltage controller tuned to control the voltage at its output. Also, a new system topology has been introduced with two wind turbines that are interconnected to multi‐winding transformer through asynchronous generators. The proposed system has been implemented with constant and variable wind speeds, and their respective results have also been analysed. The proposed scheme shows its effectiveness by theoretical calculations, verified by simulation and experimental results. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献