基于OpenCV的圆形标记点的提取

文章提出了一种基于OpenCV的圆形标记点的提取方法,通过对图像进行平滑处理、边缘检测、轮廓查找、几何约束以及椭圆拟合,实现圆形标记点的提取,求出中心坐标.该方法主要应用于多视点云拼接以及其他有关圆形物体识别的场合.实验结果证明,该方法能快速有效的提取圆形标记点.     

有侧吹风环流料封阀水平孔口流动阻力特性

引　言循环流化床锅炉由于具有诸多优点 ,使其在国内外得以迅速发展 .然而在已投运的循环流化床锅炉中也暴露出一些亟待完善解决的问题 .环流料封阀是目前在循环流化床锅炉上应用最多的一种固体颗粒回送控制装置 .实际运行表明 ,这种返料装置结构简单、运行可靠 ,能够很好地满足稳定返料和密封的要求 ,是一种很有发展前途的返料器 .但它缺乏对循环物料的调节和控制能力 ,返料室有时会出现局部流化不良从而导致结焦 .试验研究表明[1～ 3],只要环流料封阀在结构上设计合理 ,它就能像Ｌ阀一样 ,对固体颗粒循环量具有调节和控制作用 ,且可在较宽…     

d-Penicillamine2,5-enkephalin作为底物考察三明治大鼠原代肝细胞的转运体功能研究

目的: 通过液相色谱-串联质谱(LC/MS/MS)法测定d-Penicillamine2,5-enkephalin (DPDPE)在肝细胞和Hank's缓冲液中的浓度,研究DPDPE作为底物用于“三明治”培养大鼠原代肝细胞(SCRH)的转运体功能。方法: 将SCRH与溶于Hank's缓冲液的DPDPE共温孵,采用LC/MS/MS法测定细胞内蓄积的和外排到缓冲液中的DPDPE浓度,用于评价模型药物α-萘异硫氰酸酯(ANIT)对参与DPDPE摄取的Oatp和负责DPDPE外排的Mrp2的转运功能的影响。结果: 建立的样品处理和测定方法能够满足体外转运实验需要的灵敏度和线性范围,在Hank's缓冲液中线性范围为0.5~50 ng/mL(r²=0.9995),在细胞基质中线性范围为 0.1~5 ng/well(r²=0.9997),样品处理操作简便。日内日间精密度(RSD)均小于15%;提取回收率大于65%。ANIT可抑制DPDPE在SCRH胆管侧的外排及基底侧的摄取,对基底侧外排无显著影响。结论: 该分析方法选择性好,灵敏度高,操作简便,并成功地用于将DPDPE作为探针底物研究SCRH模型上转运体的功能。     

葡萄糖和胰岛素调节三明治肝细胞中胆酸相关转运体表达及功能

目的: 测定葡萄糖、胰岛素等糖尿病血糖中的异常因素对体外培养肝细胞中胆汁酸相关转运体表达及功能的影响。方法: 分离并用三明治法培养大鼠原代肝细胞,用不同浓度葡萄糖或胰岛素培养 72 h,使用胆盐类似物Cholyl-lysyl-fluorescein(CLF)进行摄取转运体钠/胆汁酸共转运体(Na⁺/bile acid cotransporter, ntcp)及外排转运体胆盐外排泵(Bile salt export pump, bsep)转运体功能学实验,并用荧光定量PCR法测定胆汁酸相关转运体ntcp、bsep及调节胆汁酸代谢转运的核受体(Farnesoid X receptor,fxr)的mRNA表达变化。结果: 高浓度的葡萄糖和胰岛素均使CLF的胆泡排泄减少。高糖和高胰岛素均能够降低ntcp的表达(3~4倍),增加bsep的表达(约2倍),高糖能够降低fxr的水平(约 0.5 倍),高胰岛素增加fxr的水平(约 1.5 倍)。结论: 葡萄糖和胰岛素水平异常均会对胆汁酸相关转运体表达和功能造成影响;三明治原代肝细胞可用于胆酸和药物肝胆分布研究。     

On a eulerian and lagrangian combined model in dense particle-laden riser flow

A new numerical model using both Eulerian and Lagrangian coordinates, and taking account of interparticle interactions, has been developed for the study of hydrodynamic aspects of dense particle-laden rise flows. A stochastic particle dispersion model has been incorporated in the original model to describe the gas particle turbulent flows. In addition, the collisional interaction between the particles has been modeled using the kinetic theory of granular flows based on the Chapman-Enskog theory of dense gases. A comparison with the experimental results of Miller and Gidspow (1992) shows reasonably good agreement. The present model may provide a useful approach for predicting the complex hydrodynamic behaviour of fluidized bed systems.     

绿色木霉合成几丁质酶条件的研究

绿色木霉ＺＵ０１１在以０．５％几丁质为唯一碳源的培养基中几丁质酶活达到０．１２４ＩＵ／ｍＬ。几丁质酶合成的最佳碳源和诱导物为几丁质。在一定范围内啬２基中几丁质浓度,葡萄糖浓度,微量元素盐浓度和碳氮比都能提高几丁质酶活。胆汁酸和吐温８０作为表面活性剂能显著提高几丁质酶活。     

毛皮类物料干燥特性模拟试验研究

采用正交试验法对不同干燥工况下毛皮物料干燥特性进行试验，给出了干燥曲线和干燥速率曲线，建立了干燥特性方程。试验方法具有通用性。通过正交分析，以干燥时间和能耗为目标给出最佳干燥工况。     

Rheological properties of oil sand slurries and the effect on viscosity of blending with coal

Oil sand slurries (OSSs) were prepared using four additives and their slurrying properties were studied. Results showed that OSSs exhibited an obvious shear-thinning flow behavior. Among the four additives, calcium lignosulfonate and petroleum sulfonate exhibited better viscosity-reducing effects. The rheological behaviors of the OSSs were fitted by yield-power law flow model, indicating high yield stress of OSSs. This resulted in a poor liquidity and better stability. In addition, adding coal in the OSS could increase the fixed-viscosity solid concentration.     

Effects of the physical and chemical properties of petroleum coke on its slurryability

In this study,the effects of particle size distribution (PSD),chemical composition and pore structure of petroleum coke on the slurryability of petroleum coke water slurry (PCWS) were investigated.Four...     

Improvement of hydrogen production by over-expression of a hydrogen-promoting protein gene in Enterobacter cloacae

The gene of a hydrogen-promoting protein (which we term HPP) from Enterobacter cloacae IIT-BT 08 was cloned and over-expressed in E. cloacae CICC10017 for the first time in this study, and the overall hydrogen yield was greatly improved using the recombinant strain. A recombinant plasmid containing the gene in-frame with Glutathione-S-Transferase (GST) gene was transformed into a hydrogen producing strain of E. cloacae CICC10017 to produce a GST-fusion protein. SDS-PAGE and western blot analysis confirm the successful expression of the GST-tagged protein. An in vitro assay of cell lysates indicates hydrogenase activity of the recombinant strain is 534.78 ± 18.51 ml/(g-DW·h), nearly 2-fold higher than the wild strain. The hydrogen yield of the recombinant strain is 2.55 ± 0.1 mol/mol-glucose, also 2-fold higher than the wild strain. The recombinant strain produces more acetate and butyrate during hydrogen fermentation, but less ethanol, due to the higher hydrogenase activity with the over-expression of the hydrogen-promoting protein. Together, the results demonstrate that successful expression of a single structural gene improves the overall yield of hydrogen by directing metabolic fluxes away from formation of products that compete for NADH.