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91.
At some stages of development, it is impossible to identify the porcine nodular worms Oesophagostomum dentatum and O. quadrispinulatum to the species level using morphological parameters. A molecular approach utilizing genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal (r) DNA was developed to overcome this limitation. The ITS-2 sequence of each species was determined, and specific oligonucleotide primers were designed to regions of greatest sequence difference between the species. Utilizing these primers, rapid PCR procedures were developed for the specific amplification of DNA of O. dentatum or O. quadrispinulatum, which are now used routinely to monitor the purity of larval cultures and to confirm the identity of larvae derived from the intestine or faeces. The application of specific PCR has major implications for studying the population biology of nodular worms in the pig model.  相似文献   
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Discusses repression in bilingual analyses, including repressed memories, transference, and dreams, as well as the whole associative process. As certain memories are more accessible in one language than the other, repression for bilingual individuals may be partly a function of linguistic accessibility. The concept of repression will therefore need to be reassessed in the context of these observations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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介绍根据三角形重心定理结合现场实际情况用几何作图方法确定连续自动配料系统中称重料斗的实际重心及弹性受力元件的安装位置,使两者尽量重合于同一垂直轴线,达到减少重量测量误差,提高测量精度和配料效果的目的。  相似文献   
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This article examines basic psychoanalytic principles and their applications to the understanding and treatment of individuals not historically included in psychoanalytic formulations. It looks at the impact of culture, ethnicity, and class, but particularly poverty. The hope is to develop successful application of psychoanalytic theory and technique to the psychological problems of people living in poverty. Careful examination of their psychological reality may offer a unique opportunity to broaden vision of assessment to what constitutes dysfunctional condition, the concept of adaptation, the development of the working alliance, the nature of resistance and transference reactions, and the like. The analyst's personal discomfort, motivations, and stubborn adherence to specific theoretical and technical stances are considered the most damaging obstacles in this endeavor. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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The glucosinolates, sulforaphane, vitamin C and lutein content after several industrial cooking methods on the new kailan-hybrid broccoli and their changes during storage for 45 days at 4 °C were studied. Boiling and sous vide induced the highest glucosinolate loss (80%), while low pressure (LP) steaming, microwaving (MW) and sous vide-MW showed the lowest (40%) loss. Glucoraphanin was the most thermostable glucosinolate. Throughout their commercial life, microwaved and grilled samples showed a decrease in total glucosinolates. Generally, myrosinase activity was completely inhibited after cooking with undetected sulforaphane contents. The initial total vitamin C dropped by up to 58% after cooking and progressively decreased during storage, with sous vide-MW (92%) and microwaving (21%) showing the highest and lowest decrements, respectively. LP steaming and microwaving were the best industrial cooking methods for maintaining the glucosinolate and vitamin C contents, and enhancing up to 7.5-fold the initial lutein content.  相似文献   
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Homogenous and heterogeneous production of hydrogen via photo-dissociation of water has been achieved with visible range solar radiation without any external potential at room temperature. Potassium ferrocyanide as a solvated-electron supplier and electron exchanger in basic buffers was used as a homogenous media for hydrogen production. Aqueous colloidal solutions of p-type Fe2O3 doped with MgO (Fe1.8Mg0.2O3) or with ZnO (Fe1.9Zn0.1O3) suspensions in ferrocyanide solutions were used as heterogeneous media ...  相似文献   
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