One‐step production of 2,3‐butanediol from starch by secretory over‐expression of amylase in Klebsiella pneumoniae

BACKGROUND: 2,3‐Butanediol (2,3‐BD) is a valuable chemical that can be biosynthesized from many kinds of substrates. For commercial biological production of 2,3‐BD, it is desirable to use cheap substrate without pretreatment, such as starch. However, there have been few reports on the production of 2,3‐BD directly from starch. RESULTS: In this work, gene malS coding for α‐amylase (EC 3.2.1.1) precursor was inserted into plasmid pUC18K, and secretory over‐expression of α‐amylase was achieved by engineered Klebsiella pneumoniae. The extracellular recombinant amylase accelerated the hydrolyzation of starch, and one‐step production of 2,3‐BD from starch was carried out by engineered K. pneumoniae. A 2,3‐BD concentration of 3.8 g L^?1 and yield of 0.19 g 2,3‐BD g^?1 starch were obtained after 24 h fermentation. CONCLUSION: The one‐step production of 2,3‐BD from starch was achieved by secretory over‐expression of amylase in K. pneumoniae. This would simplify the process and reduce the production cost considerably by enabling use of starch with minimal pretreatment. Copyright © 2008 Society of Chemical Industry     

基于遗传算法的工作辊温度场参数优化模型

针对热连轧机工作辊热辊形计算中温度场模型的热交换等参数难以确定的问题 ,建立了基于遗传算法的参数优化模型 ,可以解决复杂条件下的热参数的求解问题。利用优化参数计算的轧辊温度场与实际测量结果一致。     

Distributed Rule-Regulated Spectrum Sharing

Dynamic spectrum access is a promising technique to use spectrum efficiently. Without being restricted to any prefixed spectrum bands, nodes choose operating spectrum on-demand. Such flexibility, however, makes efficient and fair spectrum access in large-scale networks a great challenge. Prior work in this area focused on explicit coordination where nodes communicate with peers to modify local spectrum allocation, and may heavily stress the communication resource. In this paper, we introduce a distributed spectrum management architecture where nodes share spectrum resource fairly by making independent actions following spectrum rules. We present five spectrum rules to regulate node behavior and maximize system fairness and spectrum utilization, and analyze the associated complexity and overhead. We show analytically and experimentally that the proposed rule-based approach achieves similar performance with the explicit coordination approach, while significantly reducing communication cost.     

Flame retardant and the degradation mechanism of high impact polystyrene/Fe-montmorillonite nanocomposites

High impact polystyrene/Fe-montmorillonite (HIPS/Fe-MMT) nanocomposites were successfully prepared by melting intercalation. The nanostructures of HIPS/Fe-MMT were testified by X-ray diffraction (XRD) and transmission electron microscope (TEM). Corresponding to pure HIPS, the thermal stability of HIPS/Fe-MMT nanocomposites was notably improved. The peaks of heat release rate (PHRR) and the mass loss rate (MLR) were significantly reduced after the formation of the HIPS/Fe-MMT nanocomposites from cone calorimetry. And nanocomposites PHRR was further lower with the increase of Fe-MMT content in the range of 1 to 5 wt%. The degradation mechanism of HIPS and HIPS/Fe-MMT nanocomposites was conducted by pyrolysis gas chromatography mass spectrometry (Py-GC-MS). And the reason of the enhancement of thermal stability maybe is that structural iron is the operative site for radical trapping in the Fe-MMT and the nanostructure enhances the interaction of the chains of the HIPS.     

对交通预测工作的几点看法

近来,随着一些重大工程的建成使用,提高了上海交通设施的服务能力,使交通格局发生了一些变化,交通状况也出现了新的特点。但同时也发现,有些新的交通设施的使用情况与规划设计存在着一定的差异。因此,有关部门开展了一些项目的后评估工作,以总结经验,并为今后工作提供依据。交     

UCT测井技术在河南油田的应用

介绍了超声波固井质量检测仪(UCT)的原理、结构及测井方法，分析了UCT测井资料的应用情况。实践表明，UCT能准确地探测套管腐蚀，直观评价水泥与套管、水泥与地层一、二界面的胶结质量。     

多序列迭加生成值的合理性判定——病态信息检测案例

论文针对目前普遍存在于各种管理性考评工作中的专家打分问题进行了研究,提出了一种特定条件下专家打分值的合理性判定模型。该方法已在某组织的实际考评工作中进行了实验与应用,取得了较好的效果,为控制专家打分随意性和病态信息的产生,提供了一种新的思路。     

轻便、操作方便自适应控制的MIG焊机——MinarcMig^TM

Kemppi公司的新型MinarcMig^TM自适应逆变器是重量很轻、功能强大、使用方便的焊机。     

提高自主创新能力，加速变频器制造业持续快速发展

创新是企业发展不竭的动力，是增强企业竞争力的关键因素。本文就变频器企业在当前激烈的市场矗虿中，如何做好自主创新工作进行了探讨。在简要阐述自主创新的重要意义后，分析了变频器企业目前发展的现状和存在的问题。针对问题提出了变频器企业在开展自主创新时，可供参考的一些基本思路和途径。