Development of fan‐shaped tracker for single particle tracking

With the development of super‐resolution fluorescence microscopy, complex dynamic processes in living cells can be observed and recorded with unprecedented temporal and spatial resolution. Single particle tracking (SPT) is the most important step to explore the relationship between the spatio‐temporal dynamics of subcellular molecules and their functions. Although previous studies have developed SPT algorithms to quantitatively analyze particle dynamics in cell, traditional tracking methods have poor performance when dealing with intersecting trajectories. This can be attributed to two main reasons: (a) they do not have point compensation process for overlapping objects; (b) they use inefficient motion prediction models. In this paper, we present a novel fan‐shaped tracker (FsT) algorithm to reconstruct the trajectories of subcellular vesicles in living cells. We proposed a customized point compensation method for overlapping objects based on the fan‐shaped motion trend of the particles. Furthermore, we validated the performance of the FsT in both simulated time‐lapse movies with variable imaging quality and in real vesicle moving images. Meanwhile, we compared the performance of FsT with other five state‐of‐the‐art tracking algorithms by using commonly defined measures. The results showed that our FsT achieves better performance in high signal‐to‐noise ratio conditions and in tracking of overlapping objects. We anticipate that our FsT method will have vast applications in tracking of moving objects in cell.     

高分辨率SAR图像目标峰值特征提取

峰值特征是进行SAR图像自动目标识别的重要特征。本文提出了SAR图像目标的通用高斯峰值模型,根据该模型提出了目标峰值提取方法。利用实测数据以及蒙特卡罗仿真结果表明:通用高斯峰值模型是实际峰值的一种精确模型,基于该模型的峰值提取方法具有良好的提取精度。     

Chirality‐Based Biosensors

The fabrication of chiral nanostructures gives rise to characteristic chiroptical activity, which can be used for chirality‐based biosensors. Great progress is made in the use of nanoassemblies for the construction of chiral nanoparticle dimers, pyramids, helices, and twisted structures, and their chiroptical activities correlate with diverse structural geometries and enantiomeric configurations. In DNA‐hybridization‐based chiral nanoassemblies, the assembly parameters, such as the components, gaps, multicomponents, and the aftergrowth of metal, can result in multiple bands and enhanced chiroptical effects. Based on known chiral nanostructures, the existing chiral nanoassembly‐based biosensors together with their targets and signal amplification strategies are reviewed. Chirality involves multiple signals, and multitarget biosensors are introduced with newly developed chiral architectures for the accurate and reliable monitoring of biomarkers in living cells. The interactions between chiral nanoarchitectures and biosystems are also highlighted, which are important not only in the chiral dynamic switching of nano‐objects for biomonitoring, but also in manipulating cell growth, proliferation, and adhesion. The future perspectives on chiral fabrication and its use in biosensors are also comprehensively discussed.     

基于证据理论的雷达辐射源特征权重提取

为了更加准确、合理地提取雷达辐射源载频、重复频率、脉宽等特征参数的权重,文章提出一种新算法,根据侦测数据的可信度、识别库参数的自身特征分别给出基本概率赋值,再从决策层给出不同融合系数结合D—s证据理论来提取特征权重。最后举例说明了该算法的演算过程。     

一种BCH码的新型译码方法在TPS信息提取中的应用

针对DVB-T数字电视接收机中TPS信息提取使用的BCH(127,113,t=2)码的(67,53,t=2)缩短码提出了一种新的译码算法,并给出了DVB-T接收芯片中应用该译码算法的TPS信息提取模块的FPGA实现.该算法与传统的BCH译码算法相比,它可同时纠正随机和突发错误,提高纠错能力,并且具有译码速度块,硬件实现复杂度低等优点.     

密钥托管技术分析

介绍了密钥托管的定义、分类和使用以及美国密钥托管标准,分析了密钥托管的几种方法,认为芯片的防拆性和公钥密码在密钥托管中起很好的作用。     

校际共享实验教学中心建设初探——以内江市高校为例

高校普遍存在实验室资源配置重复、使用率低、专业实训教师短缺等问题。文章借鉴欧洲“流动制”教学方式与经验,提出建立校际共享实验教学中心。该中心充分发挥各校的优势实验教学平台和资源,建立共享机制与互认机制,配备专业实训教师,如此便能让学生能够充分享用优秀教育资源,以大力提升大学毕业生社会竞争力和高校办学实力。     

认知无线Mesh网络跨层设计研究

传统网络的分层设计不能满足具有特殊QoS需求的无线宽带业务,无法应对动态频谱接入的无线通信环境。采用联合开放、架构灵活的跨层设计研究无线Mesh网络接入技术日渐成为热点。本文首先介绍了跨层设计的起源、分类,然后分析了认知无线Mesh网络跨层研究的挑战、难点及最新进展,并提出利用跨层设计实现路由及频谱管理的一种构想,最后展望了认知无线Mesh网络跨层研究的新方向。     

70%稻瘟灵可湿性粉剂的研制

制备了国内外文献未见报道的70%稻瘟灵可湿性粉剂(WP),对该剂型的配方组成、加工工艺、贮存稳定性进行了研究,并对其各项性能进行了测试,确定了优惠配方。实验结果表明,该产品的各项指标均符合可湿性粉剂标准的要求,悬浮率在90%以上,润湿时间在1min以内,在40±2℃下贮存60天后的分解率小于5%。     

Comprehensive Analysis of hsa-miR-654-5p’s Tumor-Suppressing Functions

The pivotal roles of miRNAs in carcinogenesis, metastasis, and prognosis have been demonstrated recently in various cancers. This study intended to investigate the specific roles of hsa-miR-654-5p in lung cancer, which is, in general, rarely discussed. A series of closed-loop bioinformatic functional analyses were integrated with in vitro experimental validation to explore the overall biological functions and pan-cancer regulation pattern of miR-654-5p. We found that miR-654-5p abundance was significantly elevated in LUAD tissues and correlated with patients’ survival. A total of 275 potential targets of miR-654-5p were then identified and the miR-654-5p-RNF8 regulation axis was validated in vitro as a proof of concept. Furthermore, we revealed the tumor-suppressing roles of miR-654-5p and demonstrated that miR-654-5p inhibited the lung cancer cell epithelial-mesenchymal transition (EMT) process, cell proliferation, and migration using target-based, abundance-based, and ssGSEA-based bioinformatic methods and in vitro validation. Following the construction of a protein–protein interaction network, 11 highly interconnected hub genes were identified and a five-genes risk scoring model was developed to assess their potential prognostic ability. Our study does not only provide a basic miRNA-mRNA-phenotypes reference map for understanding the function of miR-654-5p in different cancers but also reveals the tumor-suppressing roles and prognostic values of miR-654-5p.