Optimal combination of media for primary isolation of Helicobacter pylori from gastric biopsy specimens

The aim of the present study was to compare eight media, four nonselective and four selective media, to determine the best combination of media for the primary isolation of Helicobacter pylori. Over a period of 5 months, mucosal antral biopsy specimens were obtained from 222 consecutive dyspeptic patients undergoing endoscopy. Biopsy samples were plated in parallel on all eight media. Egg yolk emulsion agar (EYE), Skirrow's medium, Dent's medium, and modified Thayer-Martin medium were used as selective media; modified chocolate agar (MCHOC), Triptycase soy agar (TSA), brucella agar, and brain heart infusion agar were used as nonselective media. Overall, by using these eight media, H. pylori was recovered from biopsy specimens from 114 of 222 patients, yielding an isolation rate of 51%. Comparison of all possible combinations of the eight media showed that the highest rate of isolation of H. pylori was 100% (114 of 114) with EYE-MCHOC, followed by 96.5% (110 of 114) when EYE-TSA was used. Conversely, it was found that none of the media used alone yielded a 100% rate of recovery (the maximum recovery rate was 95%, which was achieved with EYE). These results indicate that the association of EYE and MCHOC yielded the maximum recovery of H. pylori from gastric biopsy specimens. Therefore, the use of selective and nonselective media in parallel offers optimal recovery rates with only a slight increase in costs.     

Data mining in a behavioral test detects early symptoms in a model of amyotrophic lateral sclerosis.

"What's wrong with my genetically engineered animal?" is a common yet often difficult to answer question in behavioral phenotyping. We present here a method termed Pattern Array for mining movement patterns and isolating those that best capture an effect of a genetic manipulation. We demonstrate the method by searching for early motor symptoms in the open-field behavior of SOD1 mutant rats, an animal model of amyotrophic lateral sclerosis. Pattern Array was able to identify a unique motor pattern that differentiated the SOD1 mutants from the wild-type controls 2 months before disease onset. This pattern included heavy braking while moving near the arena wall but turning away from it. SOD1 mutants performed this pattern significantly less than wild-type controls in 2 independent data sets. At such early age the SOD1 mutants could not be differentiated from the controls by standard behavioral measures or by subjective observation. The early discovered symptom may enable investigators to test therapies aimed for intervention rather than remediation. Our results demonstrate the feasibility and potential of detecting subtle behavioral effects using data mining strategies. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of thermoreflectance and scanning thermal microscopy for microelectronic device temperature variation imaging: Calibration and resolution issues

We have studied temperature variations on two submicrometric dissipative structures with two different techniques. On one hand, we have used a thermoreflectance imaging technique which is a well-known non contact optical method to evaluate temperature variations but whose spatial resolution is limited by diffraction. On the other hand, we have used a scanning thermal microscope (SThM) to study the thermal behaviour of these small dissipative structures. We present qualitative results obtained by both methods and we compare their advantages and drawbacks in terms of calibration and spatial resolution for thermal measurements on microelectronic devices. In particular, we show how the thermoreflectance coefficient can become an advantage to enhance the image contrast and favour the spatial resolution.     

Seconds out

This article discusses how the UK-based company, QinetiQ Ltd., has built its reputation as one of the key players in the defense and security technology arena in its brief existence. But the challenge still remains to shed its image as the Ministry of Defense's research and test institute and firmly establish itself as a financially successful market driven company. During its first years into the commercial technology arena, QinetiQ divided its core competencies into four divisions: integrated services, sensors and electronics, knowledge and information systems, and future systems and technology. In order to develop an effective dialogue with industry and build revenue streams in the commercial sector, it appointed managing directors for the key technology streams whose key mission was to understand where the company fitted in terms of the needs of those marketplaces. With its secure base of MoD research, test and evaluation contracts, the company is in a unique position to benefit from key emerging technologies as well as opportunities across the Atlantic where driven by heightened security concerns, the company is making inroads into the lucrative US market.     

Analytical study of a solution-processed diode based on ZnO nanoparticles using multi-walled carbon nanotubes as Schottky contact

Journal of Materials Science: Materials in Electronics - The search for good electrodes processed by solution has interested several niches to produce printed solar cells, lighting emitting diodes,...     

Immunocytochemical evaluation of protein kinase C translocation to the inner nuclear matrix in 3T3 mouse fibroblasts after IGF-I treatment

The complex pathway which links the agonist-cell membrane receptor binding to the response at the genome level involves, among other elements, protein kinase C (PKC). Agonists acting at the cell membrane can affect an autonomous nuclear polyphosphoinositide signaling system inducing an activation of nuclear phosphoinositidase activity and a subsequent translocation of PKC to the nuclear region. The fine localization of PKC has been investigated by means of electron microscopy quantitative immunogold labeling in 3T3 mouse fibroblasts, mitogenically stimulated by IGF-I. The enzyme, which in untreated cells is present in the cytoplasm, except for the organelles, and in the nucleoplasm, after IGF-I treatment is reduced in the cytoplasm and almost doubled in the nucleus. The PKC isoform translocated to the nucleus is the alpha isozyme, which is found not only associated with the nuclear envelope but mainly with the interchromatin domains. By using in situ matrix preparations, PKC appears to be retained at the nuclear matrix level, both at the nuclear lamina and at the inner nuclear matrix, suggesting a direct involvement in the phosphorylation of nuclear proteins which are responsible for the regulation of DNA replication.     

Study of the thermomechanical and electrical properties of conducting composites containing natural rubber and carbon black

This work describes the preparation and characterization of composite materials obtained by the combination of natural rubber (NR) and carbon black (CB) in different percentages, aiming to improve their mechanical properties, processability, and electrical conductivity, aiming future applications as transducer in pressure sensors. The composites NR/CB were characterized through optical microscopy (OM), DC conductivity, thermal analysis using differential scanning calorimetry (DSC), dynamic mechanical thermal analysis (DMA), thermogravimetry (TGA), and stress–strain test. The electrical conductivity varied between 10^?9 and 10 S m^?1, depending on the percentage of CB in the composite. Furthermore, a linear (and reversible) dependence of the conductivity on the applied pressure between 0 and 1.6 MPa was observed for the sample with containing 80 wt % of NR and 20% of CB. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007     

On simple scheme of finite/fixed-time control design*

ABSTRACT

Control laws are designed for stabilisation of a chain of integrators of arbitrary degree in finite and fixed time. Presented control laws are obtained with use of Lyapunov function method and homogeneity concept. The present analysis is based on use of explicitly defined Lyapunov function that is the hallmark with respect to similar works. This analysis allows to get simple procedure of parameters tuning and obtain new estimates for settling-time function. The theoretical results are supported by numerical examples.     

Carbon-coated silver nanoparticles dispersed in a 2024 aluminum alloy produced by mechanical milling

Aluminum-based nanocomposites were produced by dispersing different contents of carbon-coated silver nanoparticles (Ag-C NP) into a 2024 aluminum alloy (Al₂₀₂₄). After milling nanostructures about 100 nm grain sizes were obtained. Increment in mechanical properties was observed in all composites synthesized. The reinforcement nanoparticles were distributed homogeneously into the Al₂₀₂₄ matrix by mechanical milling process. The yield strength (σ_y) and Brinell hardness (HB), of the nanocomposites increased as the nanoparticles content was increased as well.