922E型电动铲运机的研制与使用

系统论述了我国最新研制成功的922E型电动铲运机的主要参数、结构特点、与国内同类型的比较及在矿山的使用情况.     

一段转化炉竖琴管排吊簧改造及调整

针对一段蒸汽转化炉厚壁型炉管(HK-40)改薄壁型炉管(HP-Nb+M)后,因单根炉管重量减少近30%,受压恒力吊簧无调节功能,不能满足新载荷工况下的使用要求问题,于1996年和2007年两次对弹簧K(弹性系数)值进行校验。鉴于校验值与设计值无明显变化,弹簧特性还处于良好状态,从降低设备改造成本考虑,对竖琴管排承重吊簧进行改造使用,由新核定的工作载荷确定新工况下A、B吊簧改造后的冷态"O"值,并确定管排安装方法和吊簧的冷、热态调整要求。     

Properties and carbonization behavior of asphalt modified with the THF-soluble fraction of a coal liquefaction residue

This study focuses on the effects of the tetrahydrofuran-soluble fraction of direct coal liquefaction residue as a modifier to modify asphalt with formaldehyde. The properties and carbonization behavior of matrix asphalt and the modified asphalts were studied. The results indicate that the ductility properties of modified asphalts have been effectively improved. The morphology of modified asphalts without the addition of formaldehyde is a set of uniform distribution point, and it is converted from linear distribution to clustered distribution observed in fluorescence microscope images with the increase of the adding amount of formaldehyde.     

Enantioselective Construction of Cyclic Enaminone‐Based 3‐Substituted 3‐Amino‐2‐oxindole Scaffolds via Catalytic Asymmetric Additions of Isatin‐Derived Imines

The first catalytic asymmetric construction of the cyclic enaminone‐based 3‐substituted 3‐amino‐2‐oxindole scaffold with potential bioactivity has been developed via chiral phosphoric acid‐catalyzed enantioselective addition reactions of cyclic enaminones to isatin‐derived imines, which afforded a series of cyclic enaminone‐based 3‐substituted 3‐amino‐2‐oxindoles in high yields and excellent enantioselectivities (up to 99% yield, 97% ee). The investigation of the reaction mechanism suggested that it was facilitated by a dual hydrogen‐bonding activation mode between the two substrates and the chiral phosphoric acid. Besides, this method could be utilized for a large‐scale synthesis with maintained enantioselectivity. This approach will not only offer a useful method for enantioselective construction of the cyclic enaminone‐based 3‐substituted 3‐amino‐2‐oxindole scaffold, but also enrich the research on catalytic asymmetric addition reactions of isatin‐derived imines by using electron‐rich olefins as nucleophiles. More importantly, a preliminary evaluation on the cytotoxicity of some selected products revealed that two of the enantio‐pure compounds exhibited moderate to strong cytotoxicity to A549, 786‐0, ECA109 and BT474 cancer cell lines.

     

Synthesis of Polymer Nanospheres Conjugated Ce (IV) Complexes for Constructing Double Antibacterial Centers

Journal of Inorganic and Organometallic Polymers and Materials - The antimicrobial materials are rapidly emerging as a primary component in mitigation of bacterial pathogens, and functional...     

Genomic and metabolomic analysis of Bacillus licheniformis with enhanced poly-γ-glutamic acid production through atmospheric and room temperature plasma mutagenesis

Poly-γ-glutamic acid is an extracellular polymeric substance with various applications owing to its valuable properties of biodegradability, flocculating activity, water solubility, and nontoxicity. However, the ability of natural strains to produce poly-γ-glutamic acid is low. Atmospheric and room temperature plasma was applied in this study to conduct mutation breeding of Bacillus licheniformis CGMCC 2876, and a mutant strain M32 with an 11% increase in poly-γ-glutamic acid was obtained. Genome resequencing analysis identified 7 nonsynonymous mutations of ppsC encoding lipopeptide synthetase associated with poly-γ-glutamic acid metabolic pathways. From molecular docking, more binding sites and higher binding energy were speculated between the mutated plipastatin synthase subunit C and glutamate, which might contribute to the higher poly-γ-glutamic acid production. Moreover, the metabolic mechanism analysis revealed that the upregulated amino acids of M32 provided substrates for glutamate and promoted the conversion between L- and D-glutamate acids. In addition, the glycolytic pathway is enhanced, leading to a better capacity for using glucose. The maximum poly-γ-glutamic acid yield of 14.08 g·L^–1 was finally reached with 30 g·L^–1 glutamate.     

Monitoring wind effects of a landfall typhoon on a 600 m high skyscraper

This article presents the observations of the atmospheric boundary layer (ABL) and wind effects on a 600 m high skyscraper during a landfall typhoon. Wind structure and characteristics throughout the entire ABL are presented and discussed based on records from a Doppler radar wind profiler and near-ground measurements at several meteorological stations. Wind speed profiles with the feature of low-level jets and radial-distance and exposure dependences of gradient height are stressed. Afterwards, wind-induced pressures on building surfaces of the skyscraper are investigated with highlights on non-Gaussian probability distributions of negative pressures and vortex shedding occurred alternately at two sides of the building. The structural responses measured by both accelerometers and strain gauges are analyzed subsequently. Modal parameters of the skyscraper and their dependence on response amplitude are presented and discussed. This study aims to provide useful information for the wind-resistant design of skyscrapers in tropic cyclone-prone regions.     

Protein A Detection Based on Quantum Dots-Antibody Bioprobe Using Fluorescence Coupled Capillary Electrophoresis

In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.     

市政道路施工中软基加固技术的应用分析

软土基处理的质量好坏既可以对市政道路路基具有的基础承载力有着直接的影响,还可以确保市政道路建设完工后的高效运行以及安全运营。反之则会导致出现地基失稳的情况,从而使道路出现不均匀沉降以及沉降过大的情况,并严重危害着市政道路。