Micropatterned surfaces for control of cell shape, position, and function

The control of cell position and function is a fundamental focus in the development of applications ranging from cellular biosensors to tissue engineering. Using microcontact printing of self-assembled monolayers (SAMs) of alkanethiolates on gold, we manufactured substrates that contained micrometer-scale islands of extracellular matrix (ECM) separated by nonadhesive regions such that the pattern of islands determined the distribution and position of bovine and human endothelial cells. In addition, the size and geometry of the islands were shown to control cell shape. Traditional approaches to modulate cell shape, either by attaching suspended cells to microbeads of different sizes or by plating cells on substrates coated with different densities of ECM, suggested that cell shape may play an important role in control of apoptosis as well as growth. Data are presented which show how micropatterned substrates were used to definitively test this hypothesis. Progressively restricting bovine and human endothelial cell extension by culturing cells on smaller and smaller micropatterned adhesive islands regulated a transition from growth to apoptosis on a single continuum of cell spreading, thus confirming the central role of cell shape in cell function. The micropatterning technology is therefore essential not only for construction of biosurface devices but also for the investigation of the fundamental biology of cell-ECM interactions.     

A FISH technique for simultaneous detection of fluorescent R-band and in situ hybridization signals]

BACKGROUND: Because of serious adverse effects of centrally acting antitussive agents, is necessary to find new drugs with cough-suppressing activity. Medicinal herbs are a potential source of polysaccharides with high antitussive efficiency and on the other with minimal side effects. AIM: The study was to assess the antitussive action of mixture of a polysaccharides (RL) and polysaccharide-xylan (XY), both isolated from above-ground parts of Rudbeckie fulgida var. sullivantii. The observed activity was compared to those of narcotic and nonnarcotic antitussive substances used in clinical practice. METHODS: Cough was evoked by mechanical irritation of the airways in nonanaesthetized cats with chronic tracheal cannuly. The plant substances were administered perorally in the dose of 50 mg per kg body weight. RESULTS: Results indicate, that administration of RL induced a suppression of the followed cough parameters from both areas of airways (total fall in cough parameters by 46.6%). Administration of xylan induced the fall in the followed cough parameters with more significant influence on the laryngopharyngeal area of the airways (total fall in cough parameters 48.2%). CONCLUSION: Administration of RL and xylan did not achieve the effect of codeine, but had a more intensive antitussive effect than the peripherally acting droprozine and prenoxdiazine. (Fig. 3, Ref. 11.)     

酞菁光敏剂对S180移植瘤的疗效

研究了GaS_4PC,ZnS_2PC两种酞菁在激光照射下对S180肉瘤的疗效,并讨论了其他酞菁光敏剂对癌细胞的杀伤效果。     

横向光泵获得S_2紫外激光

用ＸｅＣｌ准分于激光器（３０８．１ｎｍ）横向泵浦Ｓ２激光器，又成功地实现了Ｓ２Ｂ－Ｘ态近ＵＶ波段的激光振荡。在波长３３０．９～３９０．０ｎｍ范围内观测到６条近ＵＶ激光输出。测量了有关的激光参数。     

强激光热斑形成及其稳定性研究

在论述热点跟踪及其传热机理时将靶材的比热溶、热传导系、在激光的热藕合系数均考虑成温度和时间的函数，又考虑靶材在运动时将激光辐照物体时热点的建立及其稳定情况作一动态数值模拟，为热点跟踪作准备。     

丁苯嵌段共聚物与醋酸乙烯酯本体接枝物表征的研究

本文主要对接枝共聚物SBS-g-VAC的红外光谱(IR)、核磁共振潜(NMR)、透射电镜(TEM)、扫描电镜(SEM)的表征工作进行了讨论,表明了接枝物SBS-g-VAC的存在及其结构特征。     

ℋ2-optimal design of multirate sampled-data systems

Treating causality constraints, this paper studies optimal synthesis of multirate sampled-data systems with an ℋ₂ performance criteria. An explicit solution is obtained by input-output space extensions (lifting) and frequency-domain techniques     

Talbot效应研究新进展

综述了对Ｔａｌｂｏｔ效应研究的进展，着重介绍了对它的新解释。