Characterization of mutated transforming growth factor-beta s which possess unique biological properties

Transforming growth factor-beta (TGF-beta) is a potent regulator of cell growth and differentiation. On the basis of the crystal structure of TGF-beta 2, we have designed and synthesized two mutant TGF-beta s, TGF-beta 1 (71 Trp) and TGF-beta 1 (delta 69-73). Although both of these molecules inhibited the growth of Mv1Lu mink lung epithelial cells and LS1034 colorectal cancer cells, which are affected equally by TGF-beta 1 and TGF-beta 2, TGF-beta 1 (delta 69-73) was much less potent than TGF-beta 1 or TGF-beta 1 (71 Trp) at inhibiting the growth of LS513 colorectal cancer cells which are growth-inhibited by TGF-beta 1 but not TGF-beta 2. Both TGF-beta 1 (71 Trp) and TGF-beta 1 (delta 69-73) increased levels of mRNAs for fibronectin and plasminogen activator inhibitor with Mv1Lu cells, whereas only TGF-beta 1 (71 Trp) and not TGF-beta 1 (delta 69-73) up-regulated the mRNA level of carcinoembryonic antigen in LS513 cells. The expression level of carcinoembryonic antigen mRNA in LS1034 cells was not altered by either wild-type or mutant TGF-beta s. Receptor labeling experiments demonstrated that TGF-beta 1 (71 Trp) bound with high affinity to the cell-surface receptors of Mv1Lu, LS1034, and LS513 cells while TGF-beta 1 (delta 69-73) bound effectively to the receptors of Mv1Lu and LS1034 cells but much less to the receptors on LS513 cells.(ABSTRACT TRUNCATED AT 250 WORDS)     

Corticofugal regulation of auditory sensitivity in the bat inferior colliculus

Risk factors for severe acute asthma involve the patient, the environment and the society in general. In addition, there are strong interrelationships between these factors. Personal characteristics linked to acute severe asthma include age (teenagers), denial leading to poor compliance, depression with atypical symptoms in children, and some medical features of asthma associated with increased severity. Environmental factors include airborne allergens, infections, irritants such as air pollutants and passive smoking. Life events have not been extensively studied but are likely to play a predominant role. The social setting, particularly the health care system, acts as an interface between the patient and the environment. Most all asthma deaths are avoidable and related to dysfunctions in the health care system.     

Using thermally stimulated currents to find defective capacitors

BaTiO₃ multilayer capacitors with a nominal capacitance of 3.5nF have been carefully measured using the thermally stimulated current (TSC) technique. When a 300V polarization voltage is applied at room temperature, a new TSC peak appeared in defective sample A after it has been immersed in water. No new peak was found in good-quality sample B after a similar treatment. The accelerated life test shows that sample A hasa much shorter life than sample B in terms of insulation resistance.     

RE／S值对控轧低碳Mn－Nb－RE钢硫化物成分和形貌的影响

用扫描电镜测定了含０．０４１％～０．０７６％ＲＥ的低碳Ｍｎ－Ｎｂ－ＲＥ钢中硫化物的成分、大小和形貌。ＲＥ／Ｓ值为２．８～３．０时，钢中的硫化物充分变质。     

精馏塔静态仿真：Ⅰ.静动态混合数学模型的应用

本文为了克服精馏塔静态仿真不易收敛和初值难以给定等难题，巧妙地利用隐含数值积分方法的特点，将稳态与动态数学模型耦合起来，用以求解高难度的非理想精馏塔静态仿真问题。所得静动态混俣模型大大提高了精馏塔静态仿真的鲁棒性，对于高度非理想精馏塔的仿真尤为有效。通过对某丙烯腈萃取精馏塔的静态仿真，充分证明了这种混合模型化方法的可行性。     

山洪灾害预警准确度与及时度研究

山洪灾害预警准确度与及时度是一对相互矛盾而又联系的重要指标。文章通过对这两项指标的研究和对引发灾害的降雨强度、降雨以及灾害发生的时间资料进行分析,从而获取预警系统建设的基本技术要点和参数,对把握山洪灾害预警系统的设计定位、启用时机和作用评估具有重要作用和意义。     

Application of the method of moments to acoustic scattering from fluid-filled cylinders using three different formulations

In this work, we present three different formulations, namely the pressure field integral equation formulation (PFIE), the velocity field integral equation formulation (VFIE), and the combined field integral equation formulation (CFIE) for solving acoustic scattering problems associated with two-dimensional fluid-filled bodies of arbitrary cross-section. In particular using the boundary conditions on the surface of the body, two equivalent problems, each valid for the outside and inside regions of the scatterer, are derived. By properly selecting the associated equations for these equivalent problems, the three different formulations are derived. The PFIE, VFIE, and CFIE are then solved by approximating the cylindrical cross-section by linear segments and employing the method of moments. Further, it is shown that the moment matrices generated by the PFIE and VFIE are ill-conditioned at resonant frequencies of the cylinder, whereas the CFIE generates a well-conditioned matrix at all frequencies. The solution techniques presented in this work are simple, efficient and applicable to truly arbitrary geometries. Numerical results are presented for certain canonical shapes and compared with other available data.     

小剂量辐照小鼠胸腺细胞膜流动性的变化

报道了以茈为荧光探针,探讨小剂量~(60)Co γ射线照射后小鼠胸腺细胞膜流动性的变化。结果表明,剂量在0.25-1.00Gy范围,胸腺细胞膜流动性降低与剂量之间呈明显线性关系。从0.50Gy起,辐照组荧光强度比值I_1/I_2与I_3/I_2与对照组的比较存有显著差异(P<0.05)。提示淋巴细胞膜流动性的变化可作为小剂量辐照早期效应的敏感指标。     

Prediction of compressive strength and optimization of mixture proportioning in ternary cementitious systems

This paper discussed the application of the method of the simplex-lattice design for predicting the properties of cement-based composites. On the basis of the compressive strength, its use was demonstrated on ternary paste systems composed of cement, silica fume and fly ash with constant water to binder ratio and a mass fraction of mineral admixtures not exceeding 30%. The regression model between compressive strength of paste and binder proportion was built up. The F-test method was utilized for validation of the regression model. The nonlinear programming system with upper and lower bound was solved. This allowed assessment of optimum mixture proportions and corresponding maximum compressive strength. It was shown that: (1) the 3rd-order regression model constructed by using the simplex-lattice design method could accurately predict the compressive strength in ternary paste system made of cement, silica fume and fly ash (the total mass fraction of all mineral admixtures was up to 30%); (2) to solve the nonlinear programming with the constraints of upper and lower bound played an important role in getting the optimum compressive strength and its corresponding mixture proportion at different ages; (3) the combination of the simplex-lattice design method and the optimization theory could be valuable tool for optimization of cement-based composites' properties.     

Validation and modification of WAVE spray model for diesel combustion simulation

In CFD simulation of diesel combustion, the predictive capabilities of the spray model play the most important role. Consequently, under the strict guidelines of the V&V, a method to adjust parameters of diesel spray model WAVE to accompany with the in-cylinder physical progress has been investigated in this paper. Moreover, the simulation of the spray has been validated by high-speed photography experiments and verified by the equation proposed by Hiroyasu. Finally, a modified setup for the WAVE spray model has been proposed and applied in the spray and combustion simulation for Deutz1013 diesel in different engine speed.