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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
981.
针对特种污泥PCR-DGGE图谱的优化一直是微生物分子生态学研究的重点.在反硝化抑制硫酸盐还原菌的研究中,研究厌氧硫酸盐还原污泥和反硝化污泥DNA的提取方法,应用通用引物958F/1401R扩增16S rDNA序列,探讨不同的退火温度对DGGE指纹图谱多样性影响,同时采用时间进程法对DGGE图谱进行优化.结果表明,采用PBS洗涤污泥和超声震荡有利于硫酸盐还原污泥DNA提取;采用958F/1401R为引物时,扩增片段大约500 bp,不同的退火温度对DGGE指纹图谱的多样性影响不显著;采用时间进程法可以很好地再现不同电泳阶段对样品的分离效果,最佳变性剂梯度为40%~60%,在130 V电压下,最佳电泳时间为5 h. 相似文献
982.
Effects of welding wire composition and welding process on the weld metal toughness of submerged arc welded pipeline steel 总被引:2,自引:0,他引:2
De-liang Ren ) Fu-ren Xiao) Peng Tian) Xu Wang) Bo Liao) ) Key Lab of Metastable Materials Science & Technology College of Materials Science & Engineering Yanshan University Qinhuangdao China ) Department of Materials Science Engineering Shijiazhuang Railway Institute Shijiazhuang China ) Steel Pipe Works of North China Petroleum Qing County China 《北京科技大学学报(英文版)》2009,16(1):65-70
The effects of alloying elements in welding wires and submerged arc welding process on the microstructures and low-temperature impact toughness of weld metals have been investigated.The results indicate that the optimal contents of alloying elements in welding wires can improve the low-temperature impact toughness of weld metals because the proentectoid ferrite and bainite formations can be suppressed,and the fraction of acicular ferrite increases.However,the contents of alloying elements need to vary along with the welding heat input.With the increase in welding heat input,the contents of alloying elements in welding wires need to be increased accordingly.The microstructures mainly consisting of acicular ferrite can be obtained in weld metals after four-wire submerged arc welding using the wires with a low carbon content and appropriate contents of Mn,Mo,Ti-B,Cu,Ni,and RE,resulting in the high low-temperature impact toughness of weld metals. 相似文献
983.
Antibacterial and corrosive properties of copper implanted austenitic stainless steel 总被引:1,自引:0,他引:1
Juan Xiong) Bo-fan Xu) Hong-wei Ni) ) School of Physics Electronic Technology Hubei University Wuhan China ) School of Material Metallurgy Wuhan University of Science Technology Wuhan China 《北京科技大学学报(英文版)》2009,16(3):293-298
Copper ions were implanted into austenitic stainless steel (SS) by metal vapor vacuum arc with a energy of 100 keV and an ions dose range of (0.5-8.0)×1017 cm-2. The Cu-implanted SS was annealed in an Ar atmosphere furnace. Glancing X-ray diffrac-tion (GXRD), transmission electron microscopy (TEM) and Auger electron spectroscopy (AES) were used to reveal the phase compositions, microstructures, and concentration profiles of copper ions in the implanted layer. The results show that the antibacterial property... 相似文献
984.
K.C. Mills 《北京科技大学学报(英文版)》2009,16(3):261-264
Crystallization properties play an important role in keeping a smooth running of continuous casting process and high sur-face quality of cast strands. To reduce fluorine pollution in slag, a new type of CaO-SiO2-Na2O (CSN) based mold flux was studied. The solidification and crystallization properties, including crystallization temperature, crystallization ratio and solidification minera-graphy, were measured, which were compared with the CaO-SiO2-CaF2 (GF) mold flux. The results show that the crystallization performance is equal to the high fluoride mold powder and CSN can be used for peritectic steel grades sensitive to longitudinal cracking in continuous casting. 相似文献
985.
Yue-long Bai) Jun Xu) Zhi-feng Zhang) Wei-min Mao) Hong Xu) ) General Research Institute for Non-ferrous Metals Beijing China ) School of Materials Science Engineering University of Science Technology Beijing Beijing China ) Research Center for Foundry Engineering North University of China Taiyuan China 《北京科技大学学报(英文版)》2009,16(4):422-426
The effect of injection pressure, piston velocity, and the forming temperature of semisolid slurry on the filling behavior of the semi-solid A356 aluminum alloy was investigated by simulation methods. The simulation results show that these processing parameters have an important effect on the filling behavior of the semi-solid A356 aluminum alloy. The slurry flows steadily in the cavity when the injection pressure, the piston velocity, and the forming temperature are low, but it is prone to turbulent flow w... 相似文献
986.
Inhibitory effect of Cr(Ⅵ) on activities of soil enzymes 总被引:1,自引:0,他引:1
To evaluate the influence of various Cr(VI) concentrations (0.05, 0.25, 0.50, 1.00 and 2.00 g/kg) on the activity of soil
enzymes, the activities of catalase, polyphenol oxidase, dehydrogenase, alkaline phosphatase in soils were investigated in
the incubation experiment with a period of 35 d. The results indicate that all the tested Cr(VI) concentrations significantly
inhibit dehydrogenase activity by over 70% after 35 d. The activity of alkaline phosphatase is slightly inhibited during the
whole experiment except for on the day 7. Cr(VI) has no obvious effect on the activity of catalase in soil. On the contrary,
Cr(VI) stimulates the activity of polyphenol oxidase. The results suggest that dehydrogenase activity can be used as an indicator
for assessing the severity of chromium pollution.
Foundation item: Projects(2006AA06Z374, 2007AA021304) supported by the National High-Tech Research and Development Program of China; Project(2008SK2007)
supported by the Key Program of Science and Technology of Hunan Province, China 相似文献
987.
Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate
technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supernatant
of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence
reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin
culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography
sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50×104. Its maximum activity appears at pH 5 and 55 °C. The chitinase is stable at pH 4.0–7.5 and below 45 °C.
Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China 相似文献
988.
Calcium phosphate film was prepared by electrochemical deposition technology. Subsequently, the alkaline treatment process
of calcium phosphate film in 0.1 mol/L NaOH solution was monitored on real time by the piezoelectric quartz crystal impedance
(PQCI) technique. The variations of morphology and composition for the alkaline treatment products were characterized by scanning
electron microscopy (SEM), Fourier transform infrared (FT-IR) and X-ray diffraction (XRD), respectively. The dynamic variations
of calcium phosphate can be characterized by the change of equivalent circuit parameters. The results show that the forming
process of hydroxyapatite (HA) is composed of three stages: (1) acidic calcium phosphate dissolution; (2) phase transformation;
and (3) HA formation. Furthermore, the correlative kinetic equations and parameters are obtained by fitting the static capacitance
(C
s)—time curves.
Foundation item: Project(2005CB623901) supported by the Major State Basic Research and Development Program of China 相似文献
989.
A recently proposed model coupling with the solid-fluid of the saturated sand was utilized to study the deformation band.
Based on the critical state plasticity model by Borja and Andrade, the hydraulic conductivity tensor was naturally treated
as a function of the spatial discretization matrix about the displacement and the stress field, allowing a more realistic
representation of the physical phenomenon. The fully Lagrangian form of the Darcy law was resolved by Piola algorithm, and
then the flow law was gained, leading to the implementation of a modified model of the saturated sand. Then the criterion
for the onset of localization was derived and utilized to detect instability. The constitutive model was implemented in a
finite element program coded by FORTRAN, which was used to predict the formation and development of shear bands in plane strain
compression of saturated sand. At last, the formation mechanism of the shear band was discussed. It is shown that the model
works well, and the simulation sample bifurcates at 1.18% axial strain, which is in a good qualitative agreement with the
experiment. The pore pressure greatly affects the onset and development of the deformation band, and it obviously increases
around the localization-prone regions with the direction toward the outer side of the normal of the shear band, while the
pore stress flows nearly horizontally and is distributed equally far away the shear band region.
Foundation item: Project(2006G007-C) supported by the Foundation of the Science and Technology Section of Ministry of Railway of China; Project(77206)
supported by the Excellent PhD Thesis Innovation Foundation of Central South University, China 相似文献
990.
A sink moving scheme based on local residual energy of nodes in wireless sensor networks 总被引:1,自引:1,他引:0
In the application of periodic data-gathering in sensor networks, sensor nodes located near the sink have to forward the data
received from all other nodes to the sink, which depletes their energy very quickly. A moving scheme for the sink based on
local residual energy was proposed. In the scheme, the sink periodically moves to a new location with the highest stay-value
defined by the average residual energy and the number of neighbors. The scheme can balance energy consumption and prevent
nodes around sink from draining their energy very quickly in the networks. The simulation results show that the scheme can
prolong the network lifetime by 26%–65% compared with the earlier schemes where the sink is static or moves randomly.
Foundation item: Project(60673164) supported by the National Natural Science Foundation of China; Project(20060533057) supported by the Specialized
Research Foundation for the Doctoral Program of Higher Education of China 相似文献