厌氧活性污泥PCR-DGGE图谱的优化

针对特种污泥PCR-DGGE图谱的优化一直是微生物分子生态学研究的重点.在反硝化抑制硫酸盐还原菌的研究中,研究厌氧硫酸盐还原污泥和反硝化污泥DNA的提取方法,应用通用引物958F/1401R扩增16S rDNA序列,探讨不同的退火温度对DGGE指纹图谱多样性影响,同时采用时间进程法对DGGE图谱进行优化.结果表明,采用PBS洗涤污泥和超声震荡有利于硫酸盐还原污泥DNA提取;采用958F/1401R为引物时,扩增片段大约500 bp,不同的退火温度对DGGE指纹图谱的多样性影响不显著;采用时间进程法可以很好地再现不同电泳阶段对样品的分离效果,最佳变性剂梯度为40%～60%,在130 V电压下,最佳电泳时间为5 h.     

S-P混杂纤维对混凝土长期性能与耐久性影响

采用对比的试验方法,研究并讨论了钢纤维、聚丙烯纤维及其混杂对混凝土长期性能及耐久性的影响.研究结果表明,用不同尺度、不同性质的钢纤维与聚丙烯纤维按二元混杂以总体积率0.9%掺入高性能混凝土中,明显改善了水泥混凝土的长期性能,90d干缩值减少36.6%,200d徐变度降低45.4%,并可显著提高混凝土的耐久性能,相对渗透系数降低79.03%,氯离子渗透系数降低30.5%.     

Set methods of left-turn waiting zone at signalized intersection

To maximize the number of vehicles passing by the stop-line in a cycle and improve the operation efficiency of intersection in China,the settlement of left-turn lane waiting-zone is becoming prevailing. Based on conflicting-point method,the internal mechanism of left-turn flow after stopping line was analyzed through taking postposition left-turn lane waiting-zone intersection for instance. The relationship between the first left-turn vehicle and the last vehicle of previous phase passing the conflicting point was expounded. According to the time of successive arriving of two vehicle flows at conflicting-point,the reasonable layout for waiting area of left-turn vehicles was researched when the clearance index was less than 0. The results suggest that the appropriate layout for waiting area of left-turning vehicles can improve the operation efficiency of intersections.     

An improvement to the simulation of phase-change heat-transfer during soil freezing and thawing

A new variable time step method, which is called the backwards calculating time step method, is presented in this paper. It allows numerical simulation of soil freezing and thawing while avoiding "phase change missing and overflowing". A sensitive heat capacity model is introduced through which the calculation errors are analyzed. Then the equation using the self-adjusted time step is presented and solved using finite differences. Through this equation, the time needed for a space cell to reach the phase change point temperature is calculated. Using this time allows the time step to be adjusted so that errors caused by "phase change missing and overflowing" are successfully eliminated. Above all, the obvious features of this method are an accelerated rate for adjusting the time step and simplifing the computations. An actual example proves that this method can accurately calculate the temperature fields during soil freezing and thawing. It is an improvement over traditional methods and can be widely used on com- plicated multi-dimensional phase change problems.     

米曲霉在牛乳中最适生长条件的研究

利用米曲霉对牛乳进行发酵,通过单因子及正交试验以菌丝体干重为指标对其在牛乳中的生长进行了研究．结果表明：接种量为4％,装液量为30mL,摇床转速为180r／min时菌丝体干重可达到最大．此外测得最大菌丝体干重时的蛋白质分解率可达35．6％．     

地质雷达和瑞雷波检测公路工程质量技术研究

地球物理方法的应用,使公路工程质量检测技术变得更加简捷、高效和经济,能够有效缓解当前路况检测和公路工程维护所面临的压力.在介绍了地质雷达和瑞雷波探测法的基本工作原理的基础上,将这2种探测技术应用在公路工程质量检测中,详细介绍了它们对公路厚度、压实度、抗压强度、弯沉值、含水量、脱空区段等检测的具体实施方法和步骤,并指出了相关注意事项.分析认为,随着技术的更新和设备的完善,地质雷达和瑞雷波技术能够有效地应用到公路工程的质量检测过程中,有着很好的应用前景和推广价值.     

相关分析在QC小组活动中的应用

QC小组将安阳卷烟厂2007年7月的质量报表中＂松散回潮工序后室温度过程能力指数小于1.33＂,不能满足要求的问题作为研究课题,通过现状调查,并将调查数据绘在散布图上,利用散布图了解到松散回潮工序前室温度标偏和后室温度标偏之间具有高度的线性相关,通过相关分析,确定了前室温度波动大是影响后室温度波动大的症结.通过实施相关对策使后室温度的过程能力指数满足要求.通过相关分析不仅准确地找出了问题的症结,而且提升了QC成果的水平.     

可拓集合论在专家系统知识表示中的应用

引入了可拓集合论来优化专家系统的设计过程,阐述了可拓集合论应用于专家系统的可行性,在分析知识表示的集合与分类特征的基础上,探讨了可拓集合论在知识表示中的应用,结合人工智能技术的发展趋势,对专家系统在可拓学中的应用前景做出了展望.     

虚拟样机技术在门座起重机设计中的应用

分析门座起重机设计的现状,结合虚拟样机技术、参数化建模技术实现门座起重机的三维参数化建模,并应用虚拟装配技术生成整机系统的虚拟样机模型;通过对门座起重机虚拟样机的建模分析,为起重机有限元动力学分析提供精确的模型,减少设计开发成本.