Right thoracotomy to approach mitral or tricuspid valve in reoperation

Four patients underwent mitral valve re-replacement, and required tricuspid annuloplasty in two, through a right thoracotomy. There was no major postoperative morbidity and recovery was full and uneventful. The indications for selection of this approach were isolated mitral valve disease or combined tricuspid and mitral valve disease, severe adhesion between heart and sternum on CT scan, intact aortic valve and no severe reduction of respiratory function. We recommend right thoracotomy to approach atrioventricular valves in selected situations in patients for reoperations.     

CD48 expression on leukocytes in infectious diseases: flow cytometric analysis of surface antigen

BACKGROUND: The function of CD48, one of the pan leukocyte cell surface antigens, is not yet well understood. CD48 was recently shown to enhance the CD40-mediated activating signal to B lymphocytes. As CD48 is one of the activation antigens of monocytes, neutrophils and lymphocytes, a change of its expression on the cells could be expected in infectious diseases. METHODS AND RESULTS: Leukocytes from 27 healthy controls and 97 patients with various infectious diseases were stained with anti CD48 antibody and analyzed by flow cytometry. On monocytes and neutrophils, the CD48 expression was increased in all of the patients with varicella, measles, rubella, infectious mononucleosis, streptococcus tonsillitis, sepsis and appendicitis. On lymphocytes, a significant increase of CD48 was also detected in the patients with the same diseases, except those with sepsis or appendicitis. The normalization of increased CD48 expression was confirmed on monocytes at the convalescent phase. CONCLUSION: These data suggest that CD48 expression on leukocytes reflects the disease activity of infectious diseases, especially of viral infections.     

Progress in Japan in electrical insulation at high temperatures

It is expected that the use of electrical equipment at high temperatures will increase in the future, and the demand for heat-resistant insulation that can be used at temperatures above 300°C will increase. In addition, this high temperature electrical equipment will need to be higher in voltage and have a larger capacity. Accordingly, research and development will be needed to study the high temperature behavior of insulating materials, the insulation manufacturing process, and functional evaluation methods of insulation systems. Progress in Japan in R&D of insulation systems at higher temperatures is discussed     

Precise measurement of the refractive index and optical rotatory power of a suspension by a delayed optical heterodyne technique

By using an optical heterodyne technique, we have demonstrated the detection of ballistic photons traveling through a suspension. We measured the propagation time of light in a sample with a variable optical delay line in the reference arm of an interferometer, using a superluminescent diode as a light source. The resolution and accuracy in propagation time measurement were 400 and 3 fs, respectively. The minimum detectable rotation angle of 1/10,000 deg was achieved with an integration time of 5 s. This system can measure the refractive index and optical rotatory power of the suspension in a solvent without disturbing heavy scattering from particles in the suspension, because ballistic photons are detected.     

Formaldehyde-limited cultivation of a newly isolated methylotrophic bacterium, Methylobacterium sp. MF1: enzymatic analysis related to C1 metabolism

Formaldehyde is a highly toxic compound to most living organisms. We have isolated a bacterial strain that is able to efficiently degrade formaldehyde and use it as a sole carbon source. The isolated strain was identified as Methylobacterium sp. MF1, which could grow on formaldehyde and methanol. Methylobacterium sp. MF1 was grown in batch culture using 1.2 g/l formaldehyde as a sole carbon source, which was all consumed within 200 h. In order to decompose formaldehyde more efficiently, formaldehyde-limited chemostat cultivation of Methylobacterium sp. MF1 was investigated. Formaldehyde was consumed at 1.7 g/l/d when the dilution rate was 0.012 h(-1). Under these conditions, the cell turbidity (OD610) reached 2.0. Furthermore, when the initial turbidity was adjusted to 3.0 using methanol-grown cells, continuous cultivation could be started at an initial dilution rate of 0.008 h(-1). Using these conditions, consumption of formaldehyde could be continued for at least 600 h. The enzyme activities of cells growing as a chemostat culture, using methanol or formaldehyde as a sole carbon source, were compared to that of C1 metabolism. No difference was detected in the enzyme activities for the oxidation and assimilation of C1 compounds between the two cell-free extracts. Furthermore, methanol dehydrogenase activity was detected at the same level when formaldehyde was used as a sole carbon source. These results suggest that the resistance to the toxic effects of formaldehyde exhibited by Methylobacterium sp. MF1 is related to factors other than C1 metabolism.     

The parameters evaluation and optimization of polycrystalline diamond micro-electrodischarge machining assisted by electrode tool vibration

The objective of this research is to evaluate and optimize machining parameter of tool electrode vibration on micro-electric discharge machining of polycrystalline diamond. The machining parameters evaluated are charge voltage, capacitance, and vibration of the tool electrode. An orthogonal array, signal-to-noise ratio, and analysis of variance are employed to analyze the effect of these machining parameters. The results show that by application of vibration on tool electrode in machining of polycrystalline diamond, it has significant effect up to 66.48% in increasing material removal rate without increasing surface roughness and tool electrode wear. Using Taguchi method for design of experiment, other significant effects on surface quality and tool electrode wear are also investigated. The results also show that surface roughness is mostly affected by the amount of capacitance (52.24%), and the tool electrode wear is also affected by the amount of capacitance (92.82%).     

A study of thermohydrodynamic lubrication in a circular journal bearing

An analytical method to determine the film temperature of circular journal bearings was developed, which considers the cavitation of the oil film and also recirculation and mixing of the lubricant. The results were verified experimentally. The theory is in good agreement with experiment over a wide range of operating conditions. The effects of journal speed, clearance ratio, lubricant viscosity and specific load on the bearing temperature were examined, and the following conclusions derived. (1) T_b,max, the maximum temperature on the bearing metal surface, increases considerably with the increase of speed and lubricant viscosity and with the decrease of clearance ratio. (2) With the increase of speed, the angular position of T_b,max varies considerably towards the direction of journal rotation from the upper stream side of the location of minimum film thickness, h_min, to the lower stream side of it. The change of angular position of T_b,max is greater than that of h_min. The contrary happens with decrease of the clearance ratio. (3) These characteristics of T_b,max correspond to those of the maximum temperature, T_f,max, in the oil film.     

Dystrophin, utrophin and beta-dystroglycan expression in skeletal muscle from patients with Becker muscular dystrophy

The precise localization and semiquantitative correlation of dystrophin, utrophin and beta-dystroglycan expression on the sarcolemma of skeletal muscle cells obtained from patients with Becker muscular dystrophy (BMD) was studied using three types of double immunofluorescence. Staining intensity was measured using a confocal laser microscope. Each of these proteins was identified at the same locus on the sarcolemma. The staining intensities of dystrophin and utrophin were approximately reciprocal at sarcolemmal sites where dystrophin expression was obviously observed. The staining intensity of beta-dystroglycan was strong in areas where dystrophin staining was also strong and utrophin expression was weak. Quantitative analysis revealed that the staining intensity of beta-dystroglycan minus that of dystrophin approximated the staining intensity of utrophin, indicating that the sum of dystrophin and utrophin expression corresponds to that of beta-dystroglycan. These results suggest that utrophin may compensate for dystrophin deficiency found in BMD by binding to beta-dystroglycan.     

Effect of double bonds on the γ-radiation-induced crosslinking of polyethylene

Various low-density polyethylenes ranging in initial weight-average molecular weight (M?_w) from 7600 to 589000 having a ratio of M?_w to number-average molecular weight (M?_n) of about 5 were irradiated by γ-rays in vacuo at 30°C. Gel fractions were determined and analyzed by using the equation derived by Charlesby and Pinner. The following relationships were obtained when M?_w was used as the molecular weight: where r_g represents the gel point dosage (Mrad), [C?C]₀ is the sum of the initial contents of terminal vinyl and vinylidene unsaturations (mole/g polyethylene), and q₀ and p₀ are the probabilities of crosslinking and main-chain scission per monomer unit for a unit radiation dose in Mrad, respectively. Similar relationships to the equations described above were also obtained when M?_n was used. From the results, it was concluded that terminal vinyl and vinylidene unsaturations play an important role for the gel formation in the γ-radiation-induced crosslinking of polyethylene in vacuo at room temperature.