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排序方式: 共有1622条查询结果,搜索用时 15 毫秒
91.
Watanabe M Takemasa I Kawaguchi N Miyake M Nishimura N Matsubara T Matsuo E Sekimoto M Nagai K Matsuura N Monden M Nishimura O 《Proteomics. Clinical applications》2008,2(6):925-935
In the development of novel biomarkers, the proteomic approach is advantageous because using it the cancer-associated proteins can be directly identified. We previously developed a 2-nitrobenzenesulfenyl (NBS) method to improve quantitative proteome analysis. Here, we applied this method to proteomic profiling of colorectal carcinoma (CRC) to identify novel proteins with altered expression in CRC. Each pair of tumor and normal tissue specimens from 12 CRC patients was analyzed, and approximately 5000 NBS-labeled paired peaks were quantified. Peaks with altered signal intensities (>1.5-fold) and occurring frequently in the samples (>70%) were selected, and 128 proteins were identified by MS/MS analyses as differentially expressed proteins in CRC tissues. Many proteins were newly revealed to be CRC related; 30 were reported in earlier studies of CRC. Six proteins that were up-regulated in CRC (ZYX, RAN, RCN1, AHCY, LGALS1, and VIM) were further characterized and validated by Western blot and immunohistochemistry. All six were found to be CRC-localized, either in cancer cells or in stroma cells near the cancer cells. These results indicate that the proteins identified in this study are novel candidates for CRC markers, and that the NBS method is useful in proteome mining to discover novel biomarkers. 相似文献
92.
93.
Bellis DJ Satake K Noda M Nishimura N McLeod CW 《The Science of the total environment》2002,295(1-3):91-100
The annual growth rings and bark pockets of a 250-year-old Japanese oak (Quercus crispula), collected from the Nikko National Park, Japan in 2000 AD, were analysed by ICP mass spectrometry. The annual rings, sampled in 5-year increments, recorded Pb concentrations from 0.01 to 0.1 mg kg(-1) and there was no significant change in concentration with time. In contrast, bark pocket samples dating from 1875 to the present showed a progressive increase in Pb concentration with time, from approximately 0.1 to 10 mg kg(-1). Shoots of epiphytic moss growing on the tree trunk contained 17 mg kg(-1) Pb. The bark pockets recorded historical increases in airborne Pb pollution accompanying the industrialisation of Japan, which was initiated by the opening of Japan's borders from 1854. This increase was not reflected by the annual rings. The 206Pb/207Pb isotope ratio of the bark pockets decreased from approximately 1.18 to 1.16 from 1964 to the present, indicating changes in the sources of Pb pollution. The 206Pb/207Pb isotope ratio of the moss shoots was similar to the current bark (1.16). The data showed bark pockets to be more effective than annual rings for recording historical change in airborne lead pollution. 相似文献
94.
H Wakita Y Tokura H Yagi K Nishimura F Furukawa M Takigawa 《Canadian Metallurgical Quarterly》1994,286(6):350-354
Antibody titres against Plasmodium falciparum and P. vivax were examined using the indirect fluorescent antibody test (IFAT) for 183 Gidra-speaking adults and adolescents in four ecologically different villages of lowland Papua New Guinea. The findings highlight that 1) in Gidraland P. falciparum was more prevalent than P. vivax, 2) the proportion of antibody titres of 1:64 or higher markedly differed among the villages, ranging from 35.3% to 100% for males and from 31.6% to 100% for females, and 3) in the two villages with high prevalences, these were higher among males than females. The inter-village and sex differences can be largely explained by microenvironmental conditions and behavioural patterns of the population. The population-based analyses of this study intend to contribute to a better understanding of the prevalence of malaria in human-environment settings and thus to the planning of malaria prevention. 相似文献
95.
We propose an exact algorithm for counting the models of propositional formulas in conjunctive normal form. Our algorithm is based on the detection of strong backdoor sets of bounded size; each instantiation of the variables of a strong backdoor set puts the given formula into a class of formulas for which models can be counted in polynomial time. For the backdoor set detection we utilize an efficient vertex cover algorithm applied to a certain “obstruction graph” that we associate with the given formula. This approach gives rise to a new hardness index for formulas, the clustering-width. Our algorithm runs in uniform polynomial time on formulas with bounded clustering-width. It is known that the number of models of formulas with bounded clique-width, bounded treewidth, or bounded branchwidth can be computed in polynomial time; these graph parameters are applied to formulas via certain (hyper)graphs associated with formulas. We show that clustering-width and the other parameters mentioned are incomparable: there are formulas with bounded clustering-width and arbitrarily large clique-width, treewidth, and branchwidth. Conversely, there are formulas with arbitrarily large clustering-width and bounded clique-width, treewidth, and branchwidth. 相似文献
96.
97.
In this paper, two novel structures of porous silicon (PS) light-emitting diodes (LEDs) are proposed aiming at the reduction of series resistance, Rs. The basic idea of the novel structures is to suppress the excessive growth of nanoporous silicon (nano-PS) layer that is electroluminescence- (EL-) active but highly resistive. The initial wafer of the first structure consists of a lightly-doped layer stacked on a highly-doped substrate. As a consequence of anodization, nano-PS layer is formed only in the lightly-doped layer, while meso-PS layer with moderate resistivity is formed in the highly-doped substrate. The second structure consists of alternately stacked nano- and meso-PS layers, since it is expected that multiple thin nano-PS layers connected in series are less resistive than a single thick nano-PS layer. Preliminary experimental results proved the effectiveness of these novel structures on the reduction of Rs. 相似文献
98.
Takafumi Kawaguchi Hiroyuki Nishimura Fumiaki Miwa Kazuhiro Abe Takashi Kuriyama Ikuo Narisawa 《Polymer Engineering and Science》1999,39(2):268-273
The environmental stress cracking (ESC) of acrylonitrile-butadiene-styrene (ABS) copolymer caused by a non-ionic surfactant (poly-oxyethylene alkylether) was studied by constant-load tensile creep tests and edge crack tension (ECT) tests. The fracture surfaces were investigated by a scanning electron microscope (SEM) and the morphology of the crack tip was investigated by a transmission electron microscope (TEM). It was found that the results of the creep tests performed in the non-ionic surfactant were very different from those performed in air. SEM images of the fracture surfaces showed that there were three different mechanisms of fracture and that specimens had a tendency to rupture by ESC when the stress was small. The results of the ECT tests and the TEM images showed that the change in the mechanism of the fracture was attributable to the change of morphology at the crack tip. 相似文献
99.
Takuji Yamamoto Tomofumi Nishimura Tetsuo Suzuki Hajime Tamon 《Drying Technology》2001,19(7):1319-1333
Resorcinol-formaldehyde hydrogels were synthesized by sol-gel polycondensation of resorcinol with formaldehyde in a slightly basic aqueous solution. RF cryogels, RF xerogels, and RF xerogels (MW gels) were respectively prepared from RF hydrogels by freeze drying, hot air drying, and microwave drying. Carbon cryogels, carbon xerogels and carbon MW gels were subsequently obtained by pyrolyzing RF drygels in an inert atmosphere. Freeze drying and microwave drying were effective to prepare mesoporous RF drygels and carbon gels. RF cryogels and carbon cryogels showed high mesoporosity over wide ranges of the molar ratio of resorcinol to catalyst (R/C) and the ratio of resorcinol to water (R/W) used in sol-gel polycondensation. Although RF xerogels had a few mesopores, carbon xerogels had no mesopores. RF MW gels and carbon MW gels showed mesoporosity if appropriate values of R/C and R/W were selected. 相似文献
100.
The numerical study was performed to examine the dynamic structure of methane and air jet diffusion flame with and without a duct in the transitional flow regime, that is, Re = 1000-2000. The flame sheet model of infinite chemical reaction and unit Lewis number were assumed in the simulation. The temperature dependence of the viscosity and diffusivity of mixed gases was also considered. The mechanism of vortex growth was explained by using the streaklines visualized with marker particles, and the interrelation between vortical and thermal structures is clarified for two cases with and without a duct. The presence of a duct leads to suppression of vortex growth in the streamwise direction of the methane jet, and therefore the flame spreading is significantly reduced. Furthermore, oscillatory shear stress and heat flux are discussed to understand vortex-flame interactions. It is found that in the transitional flow regime, there are similarities between oscillatory momentum and heat transport inside the flame in both cases. 相似文献