Effect of coal particle size on the coal hydrogenation reaction — In relation to the plasticity of coal

The effect of coal particle size on the catalytic hydrogenation of dry coal was investigated for three Hokkaido coals (Japan) of different ranks. It was found that the effect of coal particle size on conversion is dependent on coal rank. A greater difference in conversion with respect to coal particle size is noticed for coals of lower carbon content. The physical appearance of the products in the autoclave after the reaction suggests that the effect of particle size on conversion is dependent on the plastic properties of the heated coal sample. When the reaction proceeds with coal in the plastic state, the effect of particle size is small. As indicated by the product distribution, the plastic properties of a coal sample are related to the yield of asphaltene (hexane insoluble/benzene solubles) and/or the structural parts of original coal which yield asphaltene.     

Saccharomyces cerevisiae mata mutant cells defective in pointed projection formation in response to α-factor at high concentrations

We have isolated Saccharomyces cerevisiae MAT a mutant cells that do not form a pointed projection but elongate in response to α-factor at high concentrations. Complementation tests defined three genes, PPF1, PPF2, and PPF3 (for pointed projection formation), necessary for pointed projection formation. Allelism tests with genes known to be needed for projection formation revealed that PPF1 is identical to SPA2, while PPF2 and PPF3 are not allelic to SST2, STE2, SPA2, BEM1 or SLK1/SSP31/BCK1. The morphology of MAT a ppf mutants treated with high concentrations of α-factor is similar to that of MAT a PPF cells treated with α-factor at low concentrations. Quantitative mating tests showed that PPF2 and PPF3 are not essential for mating in either MAT a or MATα background. Monitoring of division arrest and expression of an α-factor-inducible gene revealed that mutations in the PPF genes do not affect the responses of MAT a cells to low concentrations of α-factor. Unlike wild-type cells, the ppf mutants exhibited early recovery from α-factor-induced division arrest. Furthermore, vegetatively growing ppf3-1 cells are slightly defective in cell separation of mother and daughter cells and in selection of the correct bud sites in all cell types. These results indicate that PPF2 and PPF3 are involved in the response to α-factor at high concentrations and that PPF3 is also required for proper establishment of polarity in vegetative growth.     

Schottky barrier height variation with metallurgical reactions in aluminum-titanium-gallium arsenide contacts

The behaviour of A1/Ti/n-GaAs Schottky contacts under heat treatment at around 400°C have been studied for their application to GaAs MESFETs. Barrier heights have been determined using both I-V and C-V measurements as a function of heat treatment time. Reaction products due to heat treatment have been studied by X-ray diffraction and Auger electron spectroscopy. The A1/Ti/n-GaAs Schottky contacts barrier height shows an $\text{“N”-}\text{shaped}$ variation in response to heat treatment time. A marked reduction in barrier height follows a slight increase at the initial annealing stage. The barrier height then takes a minimum value. The slight increase in barrier height at the initial stage in the heat treatment is brought about by a Ti and GaAs reaction. The marked barrier height reduction is closely correlated to formation of the compound Al₃Ti. The increase in barrier height observed after the reduction can be explained in terms of GaAlAs formation at the metal-semiconductor interface. This seems to indicate that heat treatment is essential for application of Al/Tin-GaAs Schottky contacts to practical devices.     

Ethanol and lactic acid production using sap squeezed from old oil palm trunks felled for replanting

Old oil palm trunks that had been felled for replanting were found to contain large quantities of high glucose content sap. Notably, the sap in the inner part of the trunk accounted for more than 80% of the whole trunk weight. The glucose concentration of the sap from the inner part was 85.2 g/L and decreased towards the outer part. Other sugars found in relatively low concentrations were sucrose, fructose, galactose, xylose, and rhamnose. In addition, oil palm sap was found to be rich in various kinds of amino acids, organic acids, minerals and vitamins. Based on these findings, we fermented the sap to produce ethanol using the sake brewing yeast strain, Saccharomyces cerevisiae Kyokai no.7. Ethanol was produced from the sap without the addition of nutrients, at a comparable rate and yield to the reference fermentation on YPD medium with glucose as a carbon source. Likewise, we produced lactic acid, a promising material for bio-plastics, poly-lactate, from the sap using the homolactic acid bacterium Lactobacillus lactis ATCC19435. We confirmed that sugars contained in the sap were readily converted to lactic acid with almost the same efficiency as the reference fermentation on MSR medium with glucose as a substrate. These results indicate that oil palm trunks felled for replanting are a significant resource for the production of fuel ethanol and lactic acid in palm oil-producing countries such as Malaysia and Indonesia.     

E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells

Conventionally, embryonic stem (ES) cells are cultured on a cell layer of mouse embryonic fibroblasts (MEFs) as feeder cells to support undifferentiated growth of ES cells. In this study, cell–cell interactions between mouse ES and feeder cells were artificially engineered via an epithelial cell adhesion molecule, E-cadherin, whose expression is considerable in ES cells. Mouse mesenchymal STO and NIH3T3 cells that were genetically engineered to express E-cadherin were used in ES cell cultures as feeder cells. ES cells cultured on the E-cadherin-expressing feeder cells maintained the expression of stem cell markers, alkaline phosphatase (AP), Oct3/4, Nanog and Sox2, and the efficiency of AP-positive colony formation was comparable to MEFs, and much better than parental STO and NIH3T3 cells. Furthermore, ES cells maintained on the E-cadherin-expressing feeder cells possessed the ability to differentiate into the three germ layers both in vitro and in vivo. The results indicated that E-cadherin expression in feeder cells could improve the performance of feeder cells, which may be further applicable to create new artificial feeder cell lines.     

Removal mechanisms for endocrine disrupting compounds (EDCs) in wastewater treatment - physical means, biodegradation, and chemical advanced oxidation: a review

Endocrine disrupting compounds (EDCs) are pollutants with estrogenic or androgenic activity at very low concentrations and are emerging as a major concern for water quality. Within the past few decades, more and more target chemicals were monitored as the source of estrogenic or androgenic activity in wastewater, and great endeavors have been done on the removal of EDCs in wastewater. This article reviewed removal of EDCs from three aspects, that is, physical means, biodegradation, and chemical advanced oxidation (CAO).     

Variations in discharge current waveforms and their power spectra injected from contact discharges of ESD‐gun with different arrangements

The International Electrotechnical Commission (IEC) has prescribed an immunity test (IEC61000‐4‐2) of electronic equipment against electrostatic discharges (ESDs), in which a discharge current to be injected onto equipment under test is specified. As for the waveform, however, not the whole waveform but only the rise time, the first peak, and the current amplitudes at 30 and 60 ns are given in the time domain together with their uncertainties, which are required to check on the condition that an ESD generator (ESD‐gun) shall be arranged vertically to an IEC‐recommended calibration target and its earth return wire is kept away as far as possible from a vertical ground plane (IEC standard arrangement). In this study, to clarify how arrangements of an ESD‐gun and its earth return wire affect discharge currents, we measured discharge current waveforms for contact discharges of an ESD‐gun onto an IEC calibration target with respect to various inclinations of the ESD‐gun and arrangements of its earth return wire, and also calculated their current power spectra normalized to that of the discharge current for the IEC standard arrangement. As a result, we found that inclinations of the ESD gun affect the first peak current, which increases current power spectra by 14 dB at frequencies over 300 MHz, and that arrangements of the return wire influence the current waveforms between the first and second peaks, which provides variations in power spectra by ±12 dB in the frequency range from 10 MHz to 200 MHz. This finding suggests that arrangements of an ESD‐gun and its earth return wire are likely to cause different immunity test results. It was also found that in comparison with measured discharge currents for the standard arrangement, the calculated waveform of a discharge current from a formula, which has been included in the recent standard, has a more gentle falling waveform, and produces power spectra of +15 dB in the frequency range from 10 MHz to 200 MHz and –12 dB at frequencies over 300 MHz. © 2012 Wiley Periodicals, Inc. Electr Eng Jpn, 180(1): 9–14, 2012; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.21270     

Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens

We previously reported the production of recombinant proteins using genetically manipulated chickens and quails. In this study, we constructed a retroviral vector encoding an expression cassette for a fusion protein of the extracellular domain of the human tumor necrosis factor (TNF) receptor 2 and Fc region of human IgG1 (TNFR/Fc), which is expected as an effective drug for inflammatory diseases such as rheumatoid arthritis. The concentrated viral vector was injected into developing chicken embryos. The chickens that hatched stably produced TNFR/Fc in the serum and egg yolk for six months. It appears that the fused protein is transported and accumulated into yolk from the serum, which is mediated by the Fc receptor. The protein purified from the yolk and serum inhibited the cytotoxic activity of TNF-* toward L929 cells, indicating that the protein produced by the chickens is biologically active. These results indicate the effectiveness of the recovery of Fc-fused proteins from the yolk of genetically manipulated chickens.