Determination of fatty acid composition by gas chromatography: II. Analysis with use of flame ionization detector

In this study known mixtures of four or five fatty acid methyl esters were analyzed collaboratively by gas chromatography with flame ionization detectors. The experimental data was treated statistically to examine inter- and intralaboratory scattering. More-over the effect of the use of correction factors was investigated. Even if only the specific analytical values that scattered a little were chosen, the averages of such values did not always approach the actual values. In some laboratories a sort of regularity was observed in the deviation of analytical values from real values throughout the analyses of four samples. The application of correction factors to the analytical values obtained by these laboratories resulted in a considerable decrease of interlaboratory scattering and deviation from the real values. When a constant amount of sample was injected, intralaboratory scattering was decreased, whereas interlaboratory scattering was not. Injection of large sample sizes caused deviation. From this collaborative study it was recommended that 0.5–1.0 μl of 20% solution be injected. Presented at the JOCS-AOCS Joint Meeting, Los Angeles, April 1972.     

Development and Evaluation of a Robust Sandwich Immunoassay System Detecting Serum WFA-Reactive IgA1 for Diagnosis of IgA Nephropathy

Aberrant glycosylation of IgA1 is involved in the development of IgA nephropathy (IgAN). There are many reports of IgAN markers focusing on the glycoform of IgA1. None have been clinically applied as a routine test. In this study, we established an automated sandwich immunoassay system for detecting aberrant glycosylated IgA1, using Wisteria floribunda agglutinin (WFA) and anti-IgA1 monoclonal antibody. The diagnostic performance as an IgAN marker was evaluated. The usefulness of WFA for immunoassays was investigated by lectin microarray. A reliable standard for quantitative immunoassay measurements was designed by modifying a purified IgA1 substrate. A validation study using multiple serum specimens was performed using the established WFA-antibody sandwich automated immunoassay. Lectin microarray results showed that WFA specifically recognized N-glycans of agglutinated IgA1 in IgAN patients. The constructed IgA1 standard exhibited a wide dynamic range and high reactivity. In the validation study, serum WFA-reactive IgA1 (WFA+-IgA1) differed significantly between healthy control subjects and IgAN patients. The findings indicate that WFA is a suitable lectin that specifically targets abnormal agglutinated IgA1 in serum. We also describe an automated immunoassay system for detecting WFA+-IgA1, focusing on N-glycans.     

Osteoclastic features of multinucleated giant cells responding to synthetic hydroxyapatite implanted in rat jaw bone.

Multinucleated giant cells (MGCs) that responded to synthetic hydroxyapatite (HAP) implanted in rat mandibles were studied with electron microscopy. HAP used in this study sintered at 200 degrees C (HAP200) and at 125 degrees C (HAP1250) after the synthesis by a wet method. One to three weeks after the intraosseous implantation of HAP, MGCs responding to HAP200 had not only well-developed ruffled border and the clear zone but well-developed perinuclear Golgi complex, many mitochondria and vesicles in their cytoplasms. MGCs responding to HAP1250 had the clear zone, but not the ruffled border although they showed similar cytoplasmic features to those of MGCs responding to HAP200. They merely extended short slender cytoplasmic processes to HAP1250. These results suggest that although osteoclast-like MGCs respond to HAP implanted in the bone, the development of the ruffled border-clear zone system depends on physicochemical properties of HAP.     

A 286 mm2 256 Mb DRAM with ×32 both-ends DQ

This paper describes a 256 Mb DRAM chip architecture which provides up to ×32 wide organization. In order to minimize the die size, three new techniques: an exchangeable hierarchical data line structure, an irregular sense amp layout, and a split address bus with local redrive scheme in the both-ends DQ were introduced. A chip has been developed based on the architecture with 0.25 μm CMOS technology. The chip measures 13.25 mm×21.55 mm, which is the smallest 256 Mb DRAM ever reported. A row address strobe (RAS) access time of 26 ns was obtained under 2.8 V power supply and 85°C. In addition, a 100 MHz×32 page mode operation, namely 400 M byte/s data rate, in the standard extended data output (EDO) cycle has been successfully demonstrated     

High-pressure sealed CW CO2laser with high efficiency

Discharge stabilization, long-term operation, output power characteristics, and efficiency of the high-pressure CW CO2laser have been investigated under sealed conditions. A comparison is made with low-pressure CW CO2lasers. Two types of electrode structures suitable for operations in the pressure range 100-760 torr are presented. Effects of O2and CO on the discharge stability and unsaturated gain are described. By using molecular sieve3Aas an adsorbent of water vapor, which was the most detrimental impurity, sealed operation with no decrease in output power was achieved at 0.5-1.5 kW for more than 150 h elapsed time including about 30 h of discharge time. It has been demonstrated that high efficiency can be obtained in spite of high-pressure and sealed operation. The efficiency was improved by reducing the cavity loss due to the absorption of intracavity radiation by CO2molecules in the unexcited region, and by finding the optimum of gas mixture. A maximum efficiency of 19 percent was obtained at a 1 kW power level for a 100 torr gas mixture of either CO2-CO-N2-He = 2-1-19-19 or CO2-CO-N2-He-Ar = 2-1-18-10-10. The effects of Ar and N2proportion on the unsaturated gain and saturation parameter are discussed.     

Electronically controlled high-speed wavelength-tunable femtosecondsoliton pulse generation using acoustooptic modulator

The compact system of electronically controlled high-speed wavelength-tunable femtosecond (fs) soliton pulse generation is realized for the first time using a passively mode-locked fs fiber laser, a polarization maintaining optical fiber, and an acoustooptic (A-O) modulator. The wavelength of the output pulses can be continuously tuned simply by controlling the input voltage into the A-O modulator. The wavelength of the soliton pulses can be changed at 2.5-μs intervals. Wavelength stabilization, time division wavelength multiplexed soliton pulse generation, and a wavelength scanner have been demonstrated     

Characteristics of laser diodes with a partially intermixedGaAs-AlGaAs quantum well

Laser diodes (LD's) with a partially intermixed quantum-well (QW) active layer are fabricated by Zn out-diffusion from a p-cladding layer to the QW region. The dependencies of the degree of intermixing, measured by the photoluminescence (PL) shift, on Zn concentration of the p-cladding layer (P_clad) and the Al content of the guiding layer (X_g) in a separate-confinement-heterostructure (SCH) are investigated. P_clad changes in the range from 1×10 ¹⁸ cm^-3 to 4×10¹⁸ cm^-3 and X_g changes in the range from 0.21-0.37. When P_clad is 2×10¹⁸ cm^-3 and X_g is 0.37, large bandgap energy shift of 96.1 meV is observed. The lasing wavelengths of the LD's, with the partially intermixed QW, are blue-shifted linearly with increasing P_clad and X_g. For the bandgap energy shift of 66.8 meV by PL, the threshold current density is increased by 33% from that of the nonintermixed LD. Reliability of LD's with the partially intermixed QW is investigated for the first time. In spite of a large degree of intermixing the reliability of the LD with the partially intermixed QW of 66.8 meV energy shift by PL is the same as the nonintermixed one, which is confirmed by the aging test of 2500 hours at 45°C with the output power of 1 W under CW operation     

High-power operation of AlGaAs SQW-SCH broad-area laser diodes for Nd:YAG solid-state laser pumping

AlGaAs single-quantum-well separate-confinement-heterostructure (SQW-SCH) single-stripe broad-area laser diodes (LDs) for Nd:YAG solid-state laser pumping were developed. The high-power operation of the SQW-SCH LD was demonstrated. The maximum output power under continuous wave operation at room temperature was 2.6 W in the range of the Nd:YAG absorption band. Stable operation was also confirmed for over 500 h under the condition of 25 degrees C at 1 W under continuous-wave operation.<>     

Simultaneous estimation of echo path and channel responses usingfull-duplex transmitted training data sequences

To reduce the system initialization time in high-speed full-duplex data transmission over two-wire lines, we propose an efficient full-duplex fast training algorithm which can simultaneously estimate the impulse responses of echo paths and channels at both ends. Two mutually orthogonal periodic sequences are designed and used to co-estimate the near echo, the far echo with bulk delay, and the channel response. The new algorithm can reduce the tap-setting time to half of that required by the traditional half-duplex fast training schemes. The effects of channel noise and symbol rate offset between two ends are examined in terms of both mean-square error (MSE) and signal-to-noise ratio (SNR). Both the theoretical analysis and computer simulation show that there are three degrading factors due to symbol rate offset. The SNR is inversely proportional to the sum of the estimated coefficients, the half period of the training sequences, and the square of symbol rate offset. If the far-signal-to-channel-noise ratio is 30 dB, then the degradation is significant when the symbol rate offset is more than 10 ^-4. If it is 40 dB, then the degradation is significant when the symbol rate offset is above 3×10^-5     

Strong Surface‐Termination Effect on Electroresistance in Ferroelectric Tunnel Junctions

Tunnel electroresistance in ferroelectric tunnel junctions (FTJs) has attracted considerable interest, because of a promising application to nonvolatile memories. Development of ferroelectric thin‐film devices requires atomic‐scale band‐structure engineering based on depolarization‐field effects at interfaces. By using FTJs consisting of ultrathin layers of the prototypical ferroelectric BaTiO₃, it is demonstrated that the surface termination of the ferroelectric in contact with a simple‐metal electrode critically affects properties of electroresistance. BaTiO₃ barrier‐layers with TiO₂ or BaO terminations show opposing relationships between the polarization direction and the resistance state. The resistance‐switching ratio in the junctions can be remarkably enhanced up to 10⁵% at room temperature, by artificially controlling the fraction of BaO termination. These results are explained in terms of the termination dependence of the depolarization field that is generated by a dead layer and imperfect charge screening. The findings on the mechanism of tunnel electroresistance should lead to performance improvements in the devices based on nanoscale ferroelectrics.