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141.
Kojima M Shioiri H Nogawa M Nozue M Matsumoto D Wada A Saiki Y Kiguchi K 《Journal of Bioscience and Bioengineering》2004,98(2):136-139
Kenaf was transformed by inoculation of Agrobacterium tumefaciens onto the meristems of young plants in pots. The transformation was demonstrated by three lines of evidence: a phenotypic inheritance from T(0) to T(1) plants, detection of the transgene in both T(0) and T(1) plants, and rescue of plasmids composed of T-DNA of the binary vector and flanking plant genomic DNA from T(1) plants. 相似文献
142.
Tadaomi Aikawa Taeko Hirose Itsuro Matsumoto Toshiko Morikawa Toshio Shimada Yumi Mine Yoshiki Tsujimoto Yoshiro Tsuji 《Lipids》1991,26(12):1108-1111
Administration of platelet-activating factor (PAF) to perfused adrenal increased cortisol and corticosterone secretion. With
hexadecyl PAF (C16PAF; 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine), the increase was significant at 1 nM and maximal at 10 nM. The responses to 10 nM octadecyl PAF
(C18PAF; 1-O-octadecyl-2-acetyl-sn-glycero-3-phosphocholine) were one fourth of those to 10 nM C16PAF. The addition of C16PAF to dispersed adrenal cells significantly increased cortisol and corticosterone production at 0.1 nM and 10 nM, respectively.
C16PAF was about 1000 times more potent than histamine on a molar basis in respect to cortisol response in both perfused adrenal
and dispersed adrenal cells. The results suggest that PAF induces cortisol release from dog adrenal.
Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl
Ether Lipids, Tokyo, Japan, May 1989. The present data were also reported at the VIIth International Congress on Hormonal
Steroids, Madrid, Spain, September, 1986 (J. Steroid Biochem. 25, 76S, 1986, Abstract). 相似文献
143.
Yumi Yamada Ayumu Nakashima Shigehiro Doi Naoki Ishiuchi Ryo Kanai Kisho Miyasako Takao Masaki 《International journal of molecular sciences》2021,22(8)
Mesenchymal stem cells (MSCs) are a potential therapeutic tool for preventing the progression of acute kidney injury (AKI) to chronic kidney disease (CKD). Herein, we investigated the localization and maintenance of engrafted human bone marrow-derived MSCs in rats subjected to a renal ischemia-reperfusion injury (IRI) and compared the effectiveness of two intravascular injection routes via the renal artery or inferior vena cava. Renal artery injection of MSCs was more effective than intravenous injection at reducing IRI-induced renal fibrosis. Additionally, MSCs injected through the renal artery persisted in injured kidneys for over 21 days, whereas MSCs injected through the inferior vena cava survived for less than 7 days. This difference may be attributed to the antifibrotic effects of MSCs. Interestingly, MSCs injected through the renal artery were localized primarily in glomeruli until day 3 post-IRI, and they decreased in number thereafter. In contrast, the number of MSCs localized in tubular walls, and the interstitium increased gradually until day 21 post-IRI. This localization change may be related to areas of damage caused by IRI because ischemia-induced AKI leads to tubular cell damage. Taken together, these findings suggest renal artery injection of MSCs may be useful for preventing the progression of AKI to CKD. 相似文献
144.
Lee Jinwoo Bong Hyuk Jong Kim Daeyong Lee Young-Seon Choi Yumi Lee Myoung-Gyu 《Metals and Materials International》2020,26(5):682-694
Metals and Materials International - The feasibility of room-temperature (cold) forming of peak-aged 7075 high-strength aluminum alloy (7075-T6) sheets after solution heat treatment followed by... 相似文献
145.
Carvalho AT Maia AC Ojima PY dos Santos AA Schlindwein C 《Journal of chemical ecology》2012,38(3):315-318
Flower localization in darkness is a challenging task for nocturnal pollinators. Floral scents often play a crucial role in
guiding them towards their hosts. Using common volatile compounds of floral scents, we trapped female nocturnal Megalopta-bees (Halictidae), thus uncovering olfactory cues involved in their search for floral resources. Applying a new sampling
method hereby described, we offer novel perspectives on the investigation of nocturnal bees. 相似文献
146.
The dimerization of acetoacetamide easily proceeds at room temperature in a dimethyl sulfoxide solution to afford 5-carbamoyl-4,6-dimethyl-2-pyridone. 相似文献
147.
Iha M Watanabe M Kihara Y Sugawara S Saito K Soma M Sato S Mori Y Kasuga K Kojima I Sasamura R Murata J Kobayashi M 《Reproduction (Cambridge, England)》2012,143(4):477-489
The homeoprotein EGAM1C was identified in preimplantation mouse embryos and embryonic stem (ES) cells. To explore the impact of EGAM1C on the hallmarks of mouse ES cells, MG1.19 cells stably expressing EGAM1C at levels similar to those in blastocysts were established using an episomal expression system. In the presence of leukemia inhibitory factor (+LIF), control transfectants with an empty vector formed flattened cell colonies, while Egam1c transfectants formed compacted colonies with increased E-CADHERIN expression. In Egam1c transfectants, the cellular contents of POU5F1 (OCT4), SOX2, TBX3, and NANOG increased. Cell growth was accelerated in an undifferentiated state sustained by LIF and in the course of differentiation. During clonal proliferation, EGAM1C stabilized the undifferentiated state. In adherent culture conditions, EGAM1C partly inhibited the progression of differentiation at least within a 4-day culture period in the presence of retinoic acid by preventing the downregulation of LIF signaling with a robust increase in TBX3 expression. Conversely, EGAM1C enhanced the expression of lineage marker genes Fgf5 (epiblast), T (mesoderm), Gata6 (primitive endoderm), and Cdx2 (trophectoderm) in -LIF conditions. In embryoid bodies expressing EGAM1C, the expression of marker genes for extraembryonic cell lineages, including Tpbpa (spongiotrophoblast) and Plat (parietal endoderm), increased. These results demonstrated that the ectopic expression of EGAM1C is capable of affecting the stabilization of an undifferentiated state and the progression of differentiation in MG1.19 ES cells, in addition to affecting cellular morphology and growth. 相似文献