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991.
为深入研究绿色木霉(Trichoderma viride)内切葡聚糖酶(EGⅠ)的特性与功能,利用Northern blot方法分析不同碳源条件下绿色木霉的egⅠ表达,采用RT-PCR方法从绿色木霉T4中克隆egⅠ基因的cD-NA序列,测序与生物信息学分析,并构建诱导型表达载体pYES2-egⅠ,转化酿酒酵母(Saccharomycescerevisiae)INVSc1和H158中表达.结果表明:绿色木霉在含有纤维素的液体培养基中生长,egⅠ高效表达,在秸秆中表达量最高.纤维二糖中表达量相对较低,在葡萄糖、果糖中没有表达.egⅠ基因编码框长度1 377bp,编码459个氨基酸.含有信号肽,为分泌蛋白,属于糖苷水解酶家族7,具有纤维素酶结合域(CBD)和催化域(CD).INVSc1转化子发酵培养48 h达到转录高峰期,60 h达到酶活高峰期,酶活为0.081 6 U/mL,酶活比H158转化子提高32.5%. 相似文献
992.
洪雷 《武汉理工大学学报(材料科学英文版)》2010,25(4):609-612
The conductivity property of graphite slurry infiltrated steel fiber concrete (GSIFCON) was investigated by the four-probe
method. The experimental results show that the electrical resistivity of GSIFCON decreases significantly at a specific concentration
of graphite, i e, the percolation concentration. A model was accordingly developed to explain its conductive mechanism, where graphite particles
could be served as bridge for conductive network between unconnected fiber chains. For an application, snow melting test of
GSIFCON was carried out and a simple heating power analysis was performed. The results reveal that the thermal energy produced
by GSIFCON makes snow melting effectively and the electrothermal efficiency can reach approximately 20%. 相似文献
993.
W-25Cu alloys were microwave sintered in a 2.45 GHz multimode applicator. The densification, microstructure and their dependence
on sintering mode and Fe addition were investigated in detail. Owing to the volumetric heating intrinsic in microwave processing,
a microstructure with larger W grain size in center regions was observed as against larger grain size in edge regions for
conventional sintering. Microwave sintering demonstrates its intrinsic advantages such as rapid heating rate, densification
enhancement and microstructural homogeneity; but it undesirably promotes W grain growth. Under microwave sintering, the role
of Fe addition on compact consolidation is not so substantial as under conventional sintering. Moreover Fe degrades the microstructural
quality, generating worse uniformity and coarser W grains. 相似文献
994.
Calcined kaolin/TiO2 composite particle material (CK/TCPM) was prepared with TiO2 coating on the surfaces of calcined kaolin particles by the mechano-chemical method. X-ray diffraction (XRD) and scanning
electron microscope (SEM) were used to investigate the microstructures and morphologies, respectively. The mechanism of the
mechano-chemical reaction between calcined kaolin and TiO2 was studied by infrared spectra (IR). The results show that TiO2 coats evenly on the surfaces of calcined kaolin particles by Si-O-Ti and Al-O-Ti bonds on their interfaces. The hiding power
and whiteness of CK/TCPM are 17.12 g/m2 and 95.7%, respectively, presenting its similarity to TiO2 in pigment properties. 相似文献
995.
靳鸿 《武汉理工大学学报(材料科学英文版)》2010,25(4):588-591
The behavior of resistance high-g impact of EMC (epoxy molding compound) with two package models, small outline package (POS)
and Globtop, was evaluated by experimental method used Hopkinson bar. At 120,000 g (generated in the Hopkinson bar with widths
about 70 μ s) no damage in either the POS devices or the Globtop devices was observed. In order to enhance the EMC’s ability
of resistance high-g impact, buffering effect of epoxy resin was also studied. The experimental results above all show that
EMC has a better performance of impact resistance at about 120,000 g, and epoxy resin can absorb the stress wave to have the
protected ability. The study of this paper could serve as a basis for selection packaging materials and enhance its reliability
in high-g impact environment. 相似文献
996.
刘娟红 《武汉理工大学学报(材料科学英文版)》2010,25(4):619-623
The effects of different curing systems on the properties of high volume fine mineral powder RPC (reactive powder concrete)
and the appearances of hydrates were studied. The experimental results show that dry-heating curing promotes the development
of pozzolanic reactivity of fine mineral powder; due to low cement content, 0.20 water-bind ratio and high reactive fine mineral
powder content, the strength of RPC increases by around 200% after steam curing and subsequent dry-heating curing. Scanning
electron microscopy and energy spectrum diagram showed that: after the high volume fine mineral powder RPC with 0.16 water-bind
ratio underwent steam curing and dry-heating curing, there was no significant change in the appearance of hydrates; after
the RPC with 0.20 water-bind ratio, the cement content of 150 kg/m3 and more steel slag powder underwent dry-heating curing, there was a certain change in the appearance of C-S-H, the structure
of gel was more compact and was uniformly distributed, and the Ca/Si of C-S-H gel decreased from 1.41 to around 1.20. 相似文献
997.
The effect of sodium hydroxide (NaOH) amount on phosphogypsum based cement was investigated. The mechanical performances and
hydration mechanism of the phosphogypsum-based cement with different proportions of NaOH and steel slag were analyzed based
on setting time, volume stability, strength test, XRD and SEM analyses. The experimental results show that, NaOH as an alkali
activator significantly reduces the cement setting time and improves the cement early strength. But the acceleration of hydration
proces produces coarse crystalline hydration products and the osteoporosis structure of hardened paste, which has a negative
effect on later age strength. The combination of 1% NaOH and 5% steel slag as alkali activating agents is optimal with respect
to early and later age strengths. Overdose of NaOH not only decreases the cement strength at later age, but also may cause
problem of volume stability. 相似文献
998.
张运华 《武汉理工大学学报(材料科学英文版)》2010,25(5):807-810
Aiming at the treatment of difficult-to-handle oily wastewater, the ceramics filter mediums made from the iron and steel plant
sludge were adopted to surface lipophilic modification so as to remove oil efficiently. The hydrolysis technique by FTIR and
conductivity test indicated that under acid conditions hydrogen silicon oil did not hydrolyze but release hydrogen by chemical
reaction under alkaline conditions. The results of the experiments including contact angle measurement, FTIR analysis, and
SEM analysis illustrate that a layer of lipophile coating indeed exists evenly on the surface of filter mediums after modification.
Furthermore, the hydrogen-containing silicone oil was combined through dehydration of Si-H bond with the -OH on the surface
of ceramic filter medium. 相似文献
999.
刘静 《武汉理工大学学报(材料科学英文版)》2010,25(5):753-759
ITO thin films were grown on PC(polycarbonate), PMMA(polymethyl methacrylate) and glass substrates by r.f. magnetron sputtering. The electrical, structural and chemical characteristics of ITO films were analyzed by the Hall Technique, X-ray diffraction, and X-ray photoelectron spectroscopy. XPS studies suggest that all the ITO films consist of crystalline and amorphous phases. The degree of crystallinity increases from less than 45% to more than 90% when the substrate temperature increases from 80 to 300 ℃. The In and Sn exist in the chemical state of In3+ and Sn4+, respectively, independent of substrate type and temperature. The enrichment of Sn on surface and In in body of ITO films are also revealed. And, the oxygen deficient regions exist both in surface layer and film body. For ITO films deposited under 180 ℃ , the carrier concentration are mainly provided by oxygen vacancies, and the dominant electron carrier scattering mechanism is grain boundary scattering between the crystal and the amorphous grain. For ITO films deposited over 180 ℃, the carrier concentration are provided by tin doping, and the dominant scattering mechanism transforms from grain boundary scattering between the crystal grains to ionized impurity scattering with increasing deposition temperature. 相似文献
1000.
The transparent C-doped TiO2 nanostructure films were fabricated on the silicate glass substrates by sol-gel spin-coated method. The as-prepared films
were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-visible absorption spectra (UV-vis)
and X-ray photoelectron spectroscopy (XPS). The photocatalytic activity was evaluated via the photo-catalytic oxidation of
methylene blue in aqueous under daylight irradiation at room temperature. The results show that the daylight-induced photocatalytic
activities of the as-prepared films are improved by the C-doping. The calcination temperatures significantly affect the morphology,
microstructure and photocatalytic activity of the as-prepared samples. At 723 K, the C-doped TiO2 films exhibit the highest photocatalytic activity due to the synergetic effects of good crystallization, appropriate oxygen
vacancies and strong absorption in the near UV and visible-light region. 相似文献