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111.
Y Saito T Yamagishi T Nakamura Y Ohyama H Aizawa T Suga Y Matsumura H Masuda M Kurabayashi M Kuro-o Y Nabeshima R Nagai 《Canadian Metallurgical Quarterly》1998,248(2):324-329
Viral respiratory infections cause acute airway abnormalities consisting of inflammation and physiological dysfunction in both animals and humans. It is likely that inflammatory cell products, such as cytokines, contribute substantially to viral-induced airway dysfunction. We hypothesized that imiquimod, an immune response enhancing agent that induces interferon-alpha, would attenuate the development of airway dysfunction during acute viral illness in rats. Adult Brown Norway rats were inoculated with parainfluenza type 1 (Sendai) virus or sterile vehicle, and treated with either imiquimod or water. Respiratory system resistance (Rrs), arterial oxygen tension (Pa,O2), lung viral titres and bronchoalveolar lavage (BAL) leucocyte counts were measured in anaesthetized, paralysed, ventilated rats. Virus-infected, water-treated rats had a significant decrease in Pa,O2 and had significant increases in leucocyte count and Rrs when compared to both the virus-infected, imiquimod-treated, (Pa,O2, p = 0.03; leucocyte count, p = 0.02; and Rrs, p = 0.009) and noninfected, water-treated rats (Pa,O2, p = 0.007; leucocyte count, p = 0.001; and Rrs, p = 0.01). In addition, imiquimod suppressed BAL eosinophils in both virus-infected (p = 0.02) and noninfected (p = 0.001) groups, and lowered overall virus titres (p = 0.03). Thus, both virus-induced airway inflammation and physiological dysfunction were attenuated significantly by imiquimod treatment in this animal model. By further delineating mechanisms by which infections induce airway dysfunction in animal models, more specific pharmacological interventions can be developed for the treatment of virus-induced asthma. 相似文献
112.
113.
Masuda Senichi Washizu Masao Iwadare Masahiro 《Industry Applications, IEEE Transactions on》1987,(3):474-480
A study of size and charge-dependent separation of small particles in liquid using a traveling-field-type electric curtain device is made. The principle of the separation is to make use of the spatial harmonic components of the rotating traveling field produced by such a device, the first harmonic propagating in one direction, which plays a dominant role in the region distant from the electrodes, and the second harmonic propagating in the opposite direction, which becomes dominant near the electrodes. Small particles brought into this field undergo circular motion and, as a result of field nonuniformity, are repelled from the electrodes and drift in the direction of the dominant harmonics. The lighter or more charged particles are strongly repelled from the electrodes and swept by the first harmonic, while the heavier or less charged particles can approach the electrodes and are transported by the second harmonic in the opposite direction, thus enabling separation by mass and charge. First a theoretical investigation of this method is made to clarify the operation conditions for the separation, then the experimental observations of particle motion are made and scaling laws of transport velocity with the applied voltage and frequency are confirmed. Finally, an example of a cell separator design using this method is presented. 相似文献
114.
Experimental results are presented for fundamental logic performances in a planner Gunn-effect device with two separated cathodes, which was fabricated on an epitaxial GaAs grown on a semi-insulating substrate. By selecting the polarity of input trigger pulses with respect to that of the biasing voltage, two functions, exclusive-OR (comparator) and inclusive-OR can be realised, as found by our computer simulation. 相似文献
115.
116.
Hiroki Sugiyama Moriya Ohkuma Yutaka Masuda Sun-Mee Park Akinori Ohta Masamichi Takagi 《Yeast (Chichester, England)》1995,11(1):43-52
An alkane-assimilating yeast Candida maltosa had been studied in order to establish systems suitable for biotransformation of hydrophobic compounds. However, functional expression of heterologous genes tested for this purpose had not been successful in several cases. On the other hand, it had been reported that the codon CUG, a universal leucine codon, is read as serine in C. cylindracea. The same altered codon usage had also been suggested by in vitro experiments in some Candida yeasts which are phylogenetically closely related to C. maltosa. In this study we have shown that the failure in functional expression of a heterologous gene is due to the fact that the codon CUG is read as serine in C. maltosa. This conclusion was drawn from the following experimental results: (1) when a cytochrome P450 gene of C. maltosa containing a CTG codon was expressed in C. maltosa, the corresponding amino acid was found to be serine, and not leucine; (2) a tRNA gene with an almost identical structure to that of the tRNA SerCAG gene of C. albicans could be isolated from the genome of C. maltosa; (3) the Saccharomyces cerevisiae URA3 gene, which has one CTG codon, could not complement the ura3 mutation of C. maltosa as itself, but when the CTG codon was changed to another leucine codon, CTC, the mutated gene could complement the ura3 mutation. The last result is the first example of succeeding in functional expression of a heterologous gene in Candida species having an altered codon usage by changing the CTG codon in the gene to another codon. The nucleotide sequence datum reported in this paper will appear in the GSDB, DDBJ, EMBL and NCBI nucleotide sequence databases with the Accession Number D26074. 相似文献
117.
T Masuda H Matsumura Y Oyama Y Takeda A Jitoe A Kida K Hidaka 《Canadian Metallurgical Quarterly》1998,61(5):609-613
A chemical synthesis of cassumunins A (1) and B (2), natural curcuminoid antioxidants, was developed. The synthesis was started from o-vanillin and after nine reaction steps resulted in 20% and 26% overall yields of 1 and 2, respectively. The synthetic cassumunins showed stronger protective activity than curcumin against oxidative cell death induced by hydrogen peroxide in a rat thymocyte system. 相似文献
118.
A grid electrode with closely spaced contacts was developed to measure the spatial distribution of motor unit action potentials on a skin surface. This electrode was used to estimate the configuration of innervation zones. The derived action potentials showed waveforms originating from the middle length of the muscle belly and propagating bidirectionally along the muscle fibers. The position of the innervation zones was estimated from the source of the propagation. By isolating action potentials from single motor units, the innervation zones for individual motor units were defined. Studies were performed in the biceps brachii of three normal subjects. The innervation zones comprised one to four separate regions and spread over up to 20 mm along the length of muscle fibers. The number and the spreading area of innervation zones varied with the subject and with the motor unit 相似文献
119.
Y Komiyama N Nishimura N Nishino K Okuda M Munakata C Kosaka M Masuda H Takahashi 《Canadian Metallurgical Quarterly》1998,20(5-6):683-690
Ouabainlike factors are thought to be a kind of important modulators of salt and water metabolism in essential hypertension. We purified the binding-protein of ouabain (OBP) from human plasma. The amino-terminal sequence of OBP from human plasma, (NH2-TLGQPREPQVYTLPPXREEM-), indicated that OBP is the carboxy-terminal fragment (14.4 kDa by SDS-PAGE) from T218 of IgG2 heavy chain and from A221 of the IgG1 heavy chain constant region. Moreover, plasmin-cleaved Fc fragment (pFc) of IgG possessed the ouabain-binding activity by the gel-filtration method of pFc and authentic ouabain mixture, whereas neither intact, aggregate, nor papain-cleaved Fc fragment did. The amino-terminal sequence of pFc was NH2-THTXPPXPAPELLGGPXVFL-, and this sequence corresponded to the T105 to L125 fragment of the IgG1 heavy chain constant region. The growth of cultured THP-1 cells were arrested in the dose-dependent manner by ouabain, which was inhibited by the addition of 20 microg/mL of pFc. These results suggested that plasmin-cleaved Fc of human IgG is one of the binding protein of ouabain/ouabainlike factor(s) in human plasma. 相似文献
120.
The use of epoxy patch grafts for the repair of experimentally-created diaphragmatic defects in dogs
H Matsumoto Y Oguchi Y Miyake Y Masuda S Masada Y Kuno I Shibahara K Takashima H Yamane S Yamagata Y Noishiki Y Yamane 《Canadian Metallurgical Quarterly》1996,58(7):685-687
Canine pericardium which had been treated with polyepoxy compounds (Denacol EX-313) was used as a patch graft for the correction of experimentally-created diaphragmatic defects in five dogs belonging to the same litter. Clinical, macroscopic and histological examinations were conducted every month up to five months after suturing of the patch graft. Clinical examination of the patch graft showed no apparent abnormalities. Macroscopic examination conducted during autopsy showed that the patch graft maintained adequate elasticity for five months after suturing, the surface of the patch graft was covered with a thin membrane and neovascularization was observed. Histological examination showed that the surface of the patch graft was covered with a thin membrane. Inflammatory tissue reactions were observed at one month, but gradually decreased from the second month onwards. In addition, the patch graft had excellent tissue affinity. 相似文献