尾静脉注射方法考察Stearic-PEI/SPIO 纳米基因载体的基因转染性能及磁共振可视化效果研究

实验应用硬脂酸-聚乙烯亚胺包裹超顺磁氧化铁(Superparamagnetic Iron Oxide,SPIO)装载目的基因构建可视化 纳米复合物,进行 BABL/c 雌性小鼠体内磁共振成像实验、组织学检测,探讨其是否能够有效地介导体内基因表达以及 产生磁共振成像(Magnetic Resonance Imaging,MRI)效果。MRI 实验结果显示,对小鼠尾静脉注射复合物 0.5 小时后进 行磁共振扫描,与注射生理盐水的对照组相比实验组 T2 加权信号明显降低,信号强度仅为对照组的 35%;48 小时后, 实验组 T2 加权信号强度略有回升,但与对照组信号强度相比仍存在显著差异。组织学结果显示可视化基因纳米复合物 能够穿过血管内皮细胞进入肝脏组织,并释放 DNA 在细胞内成功表达,但基因表达效果较差。综合 MRI 成像及组织检 查实验结果可知,该纳米颗粒具有优良的 MRI 成像性能,但通过尾静脉注射方法基因转染效率较低。     

A gain and bandwidth enhanced transimpedance preamplifier for Fourier-transform ion cyclotron resonance mass spectrometry

The nature of the ion signal from a 12-T Fourier-transform ion cyclotron resonance mass spectrometer and the electronic noise were studied to further understand the electronic detection limit. At minimal cost, a new transimpedance preamplifier was designed, computer simulated, built, and tested. The preamplifier design pushes the electronic signal-to-noise performance at room temperature to the limit, because of its enhanced tolerance of the capacitance of the detection device, lower intrinsic noise, and larger flat mid-band gain (input current noise spectral density of around 1 pA/√Hz when the transimpedance is about 85 dBΩ). The designed preamplifier has a bandwidth of ~3 kHz to 10 MHz, which corresponds to the mass-to-charge ratio, m/z, of approximately 18 to 61 k at 12 T. The transimpedance and the bandwidth can be easily adjusted by changing the value of passive components. The feedback limitation of the circuit is discussed. With the maximum possible transimpedance of 5.3 MΩ when using an 0402 surface mount resistor, the preamplifier was estimated to be able to detect ~110 charges in a single scan.     

浅谈网络安全技术在政府部门的应用

信息化时代要求信息公开和透明,这就对信息安全的管理提出了更高的要求。信息公开的安全性成为当今社会亟待解决的一大难题,网络安全的建设势在必行。为了更好地监督政府的工作就要进行信息公开,在政府信息公开的过程中一定要保证网络运行的安全。     

Nomadic Speech-Based Text Entry: A Decision Model Strategy for Improved Speech to Text Processing

Speech text entry can be problematic during ideal dictation conditions, but difficulties are magnified when external conditions deteriorate. Motion during speech is an extraordinary condition that might have detrimental effects on automatic speech recognition. This research examined speech text entry while mobile. Speech enrollment profiles were created by participants in both a seated and walking environment. Dictation tasks were also completed in both the seated and walking conditions. Although results from an earlier study suggested that completing the enrollment process under more challenging conditions may lead to improved recognition accuracy under both challenging and less challenging conditions, the current study provided contradictory results. A detailed review of error rates confirmed that some participants minimized errors by enrolling under more challenging conditions while others benefited by enrolling under less challenging conditions. Still others minimized errors when different enrollment models were used under the opposing condition. Leveraging these insights, we developed a decision model to minimize recognition error rates regardless of the conditions experienced while completing dictation tasks. When applying the model to existing data, error rates were reduced significantly but additional research is necessary to effectively validate the proposed solution.     

Exploring the Individual's Behavior on Self-Disclosure Online

Even though the ubiquity of technology can make many aspects of life more convenient, it may simultaneously raise some concerns about the privacy issues related to personal information. This research focused on the individual's behavior toward self-disclosure online and his or her cognition on personal privacy that was affected by not only the personal psychological factors but also the individual's computer knowledge and associated skills. To study how technological and psychological factors can simultaneously affect one's privacy concern and restrain behavior on disclosure, this study applied both perceived privacy and privacy concern to assess such factors as personal subjective attitude, computer self-efficacy, and perceived behavioral control to investigate the technological influence. A hypothesized model was proposed to describe the relationships among these constructs. After structural equation modeling is utilized to analyze the proposed hypotheses, the research findings showed that, although the perceived behavioral control had a negative effect on self-disclosure online, all the other hypotheses indicated a positive casual effect.     

A study of optimal viewing distance in a parallax barrier 3D display

The optimal viewing distance was proposed as a parameter for designing a parallax barrier 3D display. It can be designed based on simple geometric method and by considering the pitches of image display pixels and parallax barrier, or even including the aperture ratios of the image display pixels and parallax barrier. It can be analyzed by using ray tracing method. By considering the optical refraction index of the cover glass, the angular behavior of the system becomes more realistic; however, the geometric method is difficult to be used. We propose a revised method for estimating the view distance and angular behavior. In this paper, we have demonstrated a designated eye position (DEP) for each view and shown that multiple DEPs make a circular curve around the center of the display. We prove the new concept by comparing the optical ray tracing calculations and optical measurement.     

Distributed topology control algorithm on broadcasting in wireless sensor network

Topology control can enhance energy efficiency and prolong network lifetime for wireless sensor networks. Several studies that attempted to solve the topology control problem focused only on topology construction or maintenance. This work designs a novel distributed and reliable energy-efficient topology control (RETC) algorithm for topology construction and maintenance in real application environments. Particularly, many intermittent links and accidents may result in packet loss. A reliable topology can ensure connectivity and energy efficiency, prolonging network lifetime. Thus, in the topology construction phase, a reliable topology is generated to increase network reachable probability. In the topology maintenance phase, this work applies a novel dynamic topology maintenance scheme to balance energy consumption using a multi-level energy threshold. This topology maintenance scheme can trigger the topology construction algorithm to build a new network topology with high reachable probability when needed. Experimental results demonstrate the superiority of the RETC algorithm in terms of average energy consumption and network lifetime.     

An efficient and secure approach for a cloud collaborative editing

The demand for cloud-based collaborative editing service is rising along with the tremendously increased popularity in cloud computing. In the cloud-based collaborative editing environment, the data are stored in the cloud and able to be accessed from everywhere through every compatible device with the Internet. The information is shared with every accredited user in a group. In other words, multiple authorized users of the group are able to work on the same document and edit the document collaboratively and synchronously online. Meanwhile, during the whole collaborative editing process, the encryption technique is eventually applied to protect and secure the data. The encryption for the collaborative editing, however, could require much time to operate. To elevate the efficiency of the encryption, this study first analyzes the text editing in the collaborative service and presents a framework of the Red–Black tree, named as rbTree-Doc. The rbTree-Doc can reduce the amount of data to be encrypted. Although the trade-off for creating the Red–Black tree introduces extra cost, the experimental results of using rbTree-Doc in text editing operations, such as insertion and removal, show improved efficiency compared with other whole-document encryption strategy. Using rbTree-Doc, the efficiency is improved by 31.04% compared to that 3DES encryption is applied and by 23.94% compared to that AES encryption is applied.     

Exploring the relationship between technology acceptance model and usability test

In the past, most studies used the technology acceptance model (TAM) to survey the subjective perception of users in using information technology. The usability test was also used to assess the ease of use of user interfaces. This study introduces a conceptual framework to explore the relationship between user’s beliefs of TAM and usability testing attributes. Usability testing was conducted on an eCampus learning system with a mobile device. TAM data was collected from the participants for analyzing a possible relationship. The findings of this study reveal that TAM results contradict the usability test results in certain areas. The focus of our proposed research model is supported from the causality between perceived ease of use and usability; however, the correlation between perceived usefulness and usability remains unclear.