Ligands with affinity to specific sequences of DNA base pairs. X. Synthesis and binding of netropsin analogs, containing a chelating copper ion peptide, with DNA

An analogue of netropsin has been synthesized consisting of two N-propylpyrrolcarboxamide units linked covalently to a copper-chelating tripeptide Gly-Gly-L-His by means of two and three glycine residues. Binding to DNA and synthetic polynucleotides of netropsin analogue containing three glycine residues between Gly-Gly-L-His tripeptide and the N-end of netropsin analogue (His-Nt) has been studied. It is shown that this netropsin analogue chelates a copper ion with 1:1 stoichiometry, similar to a free Gly-Gly-L-His peptide. It is found that this netropsin analogue occupies 3 to 4 base pairs upon binding to poly(dA).poly(dT) and poly[d(AT)].poly[d(AT)] polymers, irrespective of whether it binds in Cu(2+)-ligated or unligated forms. Binding constants and binding site sizes have been calculated for netropsin analogue complexes with DNA, poly(dA).poly(dT) and poly[d(AT)].poly[d(AT)] polymers at the [Cu2+]/[His-Nt] ratio equal to 0 and 1.0. In the three-component system including His-Nt and Cu(2+)-His-Nt, cooperative effects are recognized which can be explained by heterodimer generation on interaction of His-Nt and Cu(2+)-His-Nt at adjacent binding sites.     

Ex vivo expansion with stem cell factor and interleukin-11 augments both short-term recovery posttransplant and the ability to serially transplant marrow

The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-11 (IL-11) produced a 50-fold amplification of clonogenic multipotential progenitors (CFU-A). Following such ex vivo expansion, substantially fewer cells were required to rescue lethally irradiated mice. When transplanted in cell doses above threshold for engraftment, BM cells expanded ex vivo resulted in significantly more rapid hematopoietic recovery. In a serial transplantation model, unmanipulated BM was only able to consistently sustain secondary BMT recipients, but BM expanded ex vivo has sustained quaternary BMT recipients that remain alive and well more than 140 days after 4th degree BMT. These results show augmentation of both short-term recovery posttransplant and the ability to serially transplant marrow by preincubation in culture with SCF and IL-11.     

双迭片弯曲压电陶瓷换能器及振动模态分析测量

介绍了双迭片弯曲压电陶瓷换能器的制作及边缘固定金属压电陶瓷双迭片弯曲振动，并利用有限元分析了换能器的谐振模式，采用激光多普勒干涉仪对换能器的谐振特性以及振动速度和位移分布进行了测量和分析，结果表明：换能器空载性能良好，通过有限元分析与先进测量手段的结合，能够对换能器器件的制作与分析进行指导。     

松香及其衍生物在浮选分离中的应用

介绍了松香的来源、化学组成及理化性质；讨论了松香及其衍生物在非硫化物矿浮选分离、有色金属离子的浮选分离以及从水溶液中浮选分离有机物等方面的应用情况。     

鱼雷罐喷粉预处理优化研究

在已经得到的描述鱼雷罐喷粉处理基本参数具体表达式的基础上，运用多目标非线性优化方法，对工艺操作参数进行优化。     

超声波处理石油污水的实验研究

考察了在不同空化状态下超声波对石油污水化学耗氧量(COD)的降低作用。探讨了时间、功率、pH值及温度对石油污水化学耗氧量降低作用的影响。结果表明，石油污水化学耗氧量的降低率随着时间增大而增大，随着温度、pH值的增加而减小，随着功率的增加，先增加，然后再减小。     

砼坝裂缝稳定性分析

通过比较线弹性断裂力学中的两种计算应力强度因子的方法，外推法和奇异单元法，得到相应的结论；并以某砼坝下游部位出现裂缝为例，分析在不利荷载作用下裂缝的稳定性。     

磁悬浮硬盘转子控制系统中时序电路测试技术

为提高磁悬浮硬盘转子控制系统的可靠性，提出了对时序芯片的测试方法：手工测试和计算机辅助测试；及时时序电路逻辑功能的两种检测技术；基于状态表的测试技术和自动检测技术。对于时序芯片，手工测试方法效率较低，计算机辅助测试方法适用范围更广；对于电路逻辑功能，基于状态表的测试技术操作相对困难，但无须增加额外测试电路；自动检测技术操作简单，且测试速度快，但需要增加辅助检测电路。时序电路测试技术对保证悬浮硬盘转子各组成部分协同工作十分重要。     

电路板故障诊断中神经网络信息融合技术的应用

将信息融合技术应用到导弹电子设备的电路板故障诊断之中．提出了一种基于红外热成像实验的温度故障隶属度函数构造形式以及改进的BP神经网络算法。进行了BP神经网络信息融合故障诊断的实验研究．从结果看信息融合能较好地解决电路板元件故障诊断的不确定性问题。