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101.
M Kanazawa M Yano C Namchai S Yamamoto A Ohtake M Takayanagi M Mori H Niimi 《Canadian Metallurgical Quarterly》1997,239(2):580-584
cDNAs for green fluorescent protein (GFP) and for a GFP fusion protein containing the presequence of human ornithine transcarbamylase (pOTC-GFP) were transfected into cultured human fibroblasts. GFP cDNA gave diffuse fluorescence throughout the cytoplasm and the nucleus, whereas pOTC-GFP cDNA gave mitochondria-associated fluorescence. Fluorescent mitochondrial structures could be classified into five patterns: thread-like mitochondria, fine thread-like ones, rod-like ones, granular ones, and granular ones with weak cytosolic fluorescence. pOTC-GFP mutants resulted in a loss of mitochondrial fluorescence and an appearance of weak fluorescence throughout the cytoplasm. pOTC-GFP cDNA was transfected into fibroblasts from patients with various mitochondrial diseases. Higher ratios of fibroblasts with granular mitochondria and those with fine thread-like ones were observed in a patient with Reye's syndrome and a patient with Kearns-Sayre syndrome. Weak cytosolic fluorescence was sometimes observed in fibroblasts from these patients. This method will be useful to analyze mitochondrial structural alterations and disorders of mitochondrial protein import. 相似文献
102.
103.
Cresswell M.W. Allen R.A. Guthrie W.F. Sniegowski J.J. Ghoshtagore R.N. Linholm L.W. 《Semiconductor Manufacturing, IEEE Transactions on》1998,11(2):182-193
The physical widths of reference features incorporated into electrical linewidth test structures patterned in films of monocrystalline silicon have been determined from Kelvin voltage measurements. The films in which the test structures are patterned are electrically insulated from the bulk-silicon substrate by a layer of silicon dioxide provided by SIMOX (Separation by the IMplantation of OXygen) processing. The motivation is to facilitate the development of linewidth reference materials for critical-dimension (CD) metrology-instrument calibration. The selection of the (110) orientation of the starting silicon and the orientation of the structures' features relative to the crystal lattice enable a lattice-plane-selective etch to generate reference-feature properties of rectangular cross section and atomically planar sidewalls. These properties are highly desirable for CD applications in which feature widths are certified with nanometer-level uncertainty for use by a diverse range of CD instruments. End applications include the development and calibration of new generations of CD instruments directed at controlling processes for manufacturing devices having sub-quarter-micrometer features 相似文献
104.
A new commercially available diode model is described. This unified model is capable of simulating the widest range of diode technologies of any presently available. The emphasis of this paper is on describing the model's extensive features and flexibility in the different domains of operation and is of particular interest in power applications 相似文献
105.
The capacitive idling converters derived from the Cuk, SEPIC, Zeta, and flyback topologies allow soft commutation of power switches without the need for additional circuitry, making it possible to increase the switching frequency while maintaining high efficiency 相似文献
106.
John C. Hart Gordon W. Lescinsky Daniel J. Sandin Thomas A. DeFanti Louis H. Kauffman 《The Visual computer》1993,9(7):346-355
The pipelined architecture and parallel organization of the AT&T Pixel Machine image computer are described and demonstrated with applications for the visualization of multidimensional fractals, particularly linear fractals and quaternion/ stacked Julia sets. Techniques for pushing the Pixel Machine to its peak abilities are described and apply to more recent parallel image computers as well. 相似文献
107.
Kemper A. Kilger C. Moerkotte G. 《Knowledge and Data Engineering, IEEE Transactions on》1994,6(4):587-608
View materialization is a well-known optimization technique of relational database systems. We present a similar, yet more powerful, optimization concept for object-oriented data models: function materialization. Exploiting the object-oriented paradigm-namely, classification, object identity, and encapsulation-facilitates a rather easy incorporation of function materialization into (existing) object-oriented systems. Only those types (classes) whose instances are involved in some materialization are appropriately modified and recompiled, thus leaving the remainder of the object system invariant. Furthermore, the exploitation of encapsulation (information hiding) and object identity provides for additional performance tuning measures that drastically decrease the invalidation and rematerialization overhead incurred by updates in the object base. First, it allows us to cleanly separate the object instances that are irrelevant for the materialized functions from those that are involved in the materialization of some function result, and this to penalize only those involved objects upon update. Second, the principle of information hiding facilitates fine-grained control over the invalidation of precomputed results. Based on specifications given by the data type implementor, the system can exploit operational semantics to better distinguish between update operations that invalidate a materialized result and those that require no rematerialization. The paper concludes with a quantitative analysis of function materialization based on two sample performance benchmarks obtained from our experimental object base system GOM 相似文献
108.
Solotorevsky G. Gudes E. Meisels A. 《Knowledge and Data Engineering, IEEE Transactions on》1994,6(5):681-697
A general language for specifying resource allocation and time-tabling problems is presented. The language is based on an expert system paradigm that was developed previously by the authors and that enables the solution of resource allocation problems by using experts' knowledge and heuristics. The language enables the specification of a problem in terms of resources, activities, allocation rules, and constraints, and thus provides a convenient knowledge acquisition tool. The language syntax is powerful and allows the specification of rules and constraints that are very difficult to formulate with traditional approaches, and it also supports the specification of various control and backtracking strategies. We constructed a generalized inference engine that runs compiled resource allocation problem specification language (RAPS) programs and provides all necessary control structures. This engine acts as an expert system shell and is called expert system for resource allocation (ESRA). The performance of RAPS combined with ESRA is demonstrated by analyzing its solution of a typical resource allocation problem 相似文献
109.
Graefe G. Linville A. Shapiro L.D. 《Knowledge and Data Engineering, IEEE Transactions on》1994,6(6):934-944
Efficient algorithms for processing large volumes of data are very important both for relational and new object-oriented database systems. Many query-processing operations can be implemented using sort- or hash-based algorithms, e.g. intersections, joins, and duplicate elimination. In the early relational database systems, only sort-based algorithms were employed. In the last decade, hash-based algorithms have gained acceptance and popularity, and are often considered generally superior to sort-based algorithms such as merge-join. In this article, we compare the concepts behind sort- and hash-based query-processing algorithms and conclude that (1) many dualities exist between the two types of algorithms, (2) their costs differ mostly by percentages rather than by factors, (3) several special cases exist that favor one or the other choice, and (4) there is a strong reason why both hash- and sort-based algorithms should be available in a query-processing system. Our conclusions are supported by experiments performed using the Volcano query execution engine 相似文献
110.
McKinley P.K. Xu H. Esfahanian A.-H. Ni L.M. 《Parallel and Distributed Systems, IEEE Transactions on》1994,5(12):1252-1265
Multicast communication, in which the same message is delivered from a source node to an arbitrary number of destination nodes, is being increasingly demanded in parallel computing. System supported multicast services can potentially offer improved performance, increased functionality, and simplified programming, and may in turn be used to support various higher-level operations for data movement and global process control. This paper presents efficient algorithms to implement multicast communication in wormhole-routed direct networks, in the absence of hardware multicast support, by exploiting the properties of the switching technology. Minimum-time multicast algorithms are presented for n-dimensional meshes and hypercubes that use deterministic, dimension-ordered routing of unicast messages. Both algorithms can deliver a multicast message to m-1 destinations in [log 2 m] message passing steps, while avoiding contention among the constituent unicast messages. Performance results of implementations on a 64-node nCUBE-2 hypercube and a 168-node Symult 2010 2-D mesh are given 相似文献