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FS Chen PE Di Cesare AA Kale JF Lee VH Frankel SA Stuchin JD Zuckerman 《Canadian Metallurgical Quarterly》1998,13(8):867-873
We have developed a computer program for the rapid assessment of the primary structure differences between a protein of unknown sequence and a homologous known protein. Both proteins are reduced, alkylated, and digested with the same hydrolytic agent. The unfractionated peptide mixtures are submitted to automatic sequence analysis. Based on the knowledge of the reference sequence, the program utilizes the analysis data to identify all the potential peptides present in the two mixtures, determining their primary structure, homology degree, and molecular weight calculated both as integer MH+ and average mass variables. These fingerprints allow the user to easily identify the structural differences between the two proteins and clarify possible doubts by a mass spectrometric analysis of the two mixtures. In order to verify the utility of the program, we provide an application example using the already reported data of two homologous proteins. 相似文献
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AA Il''ko 《Canadian Metallurgical Quarterly》1995,81(1):41-42
Percent Consonants Correct (PCC) was computed for spontaneous connected speech samples and responses on a single-word articulation test from 29 preterm children in preschool. Maturity of speech motor control was also assessed. Moderate intercorrelations of all measures are mentioned with regard to clinical implications. 相似文献
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Exercise tolerance in postcardiac transplantation patients versus healthy controls was studied at different intervals after the surgery using bicycle ergometry. Being low after the transplantation, exercise tolerance in the patients increased throughout the rehabilitation period but still did not reach the values characteristic for healthy subjects. Initial heart rate in the above patients was higher, while chronotropic and inotropic reserves of the transplanted heart appeared lower than in the controls. The data obtained help objectively assess the condition of the patients with transplanted heart. 相似文献