Determinants of the inhibitory action of purified 14-kDa phospholipases A2 on cell-free prothrombinase complex

It has been suggested (Kini, R. R., and Evans, H. J. (1987) J. Biol. Chem. 262, 14402-14407) that the anticoagulant activity of members of the 14-kDa phospholipase A2 (PLA2) family depends on the presence of basic residues within a variable surface region (residues 54-77) distinct from both the conserved catalytic machinery and surface sites mediating the antibacterial action of these enzymes (see Weiss, J., Inada, M., Elsbach, P., and Crowl, R. M. (1994) J. Biol. Chem. 269, 26331-26337). To further define the determinants of the anticoagulant activity of PLA2, we have analyzed the inhibitory effects of purified native and recombinant PLA2 on cell-free prothrombinase. Both native and recombinant wild-type pig pancreas (net charge -1) and human "secretory" PLA2 (net charge +15) produced similar dose-dependent inhibition of prothrombinase activity that was significantly less potent than a toxic PLA2 purified from snake venom. Site-specific mutations that either increased or decreased PLA2 activity toward bactericidal/permeability-increasing protein-treated Escherichia coli by up to 50-fold had no effect on antiprothrombinase activity. In contrast, substitution of Arg for Asp-59/Gly for Ser-60 in the pig PLA2 increased antiprothrombinase activity by 5-10-fold without affecting catalytic activity toward a range of phospholipid substrates or antibacterial activity. Comparison of antiprothrombinase activity of catalytically active and inactive forms of the PLA2 and under a range of phospholipid conditions revealed that the potent antiprothrombinase activity of native toxic venom PLA2 and of the D59R.S60G mutant pancreatic PLA2 reflect combined catalytic and noncatalytic actions, the latter apparently dependent on basic residues at discrete surface sites in the enzyme.     

Iterative optimization of high-affinity proteases inhibitors using phage display. 1. Plasmin

We generated a series of libraries having variants of the first Kunitz domain of human lipoprotein-associated coagulation inhibitor (LACI-D1, also known as tissue-factor pathway inhibitor-I) displayed on bacteriophage M13 as pIII-fusions. We varied LACI-DI iteratively in two regions: the P1 region (positions 10-21) and the "second loop", (positions 31-39), which together form one end of the domain. Display-phage library Lib#1 allows 31 200 amino-acid sequences in P1 region (residues 13, 16-19). Preliminary, we screened Lib#1 against human plasmin (PLA, EC 3.4.21.7) immobolized on agarose to enrich for phage displaying variants with PLA affinity. We introduced a 1600-fold increase in second-loop diversity (residues 31, 32, 34, 39) into the population of selectants from Lib#1, yielding Lib#2. Lib#2 (allowing approximately 50 million amino-acid sequences) was screened against PLA-agarose to isolate highest affinity binders. Protein EPI-P211, derived from the best isolate of Lib#2, inhibits PLA with Ki = 2 nM (at least 500-fold better than LACI-D1) and with high specificity. We used amino-acid sequences of PLA-binding selectants to design a PLA-biased library (Lib#3) which we screened against PLA. The protein EPI-P302 (derived from the best binder obtained from Lib#3) has Ki for PLA inhibition of 87 pM, which is 25-fold better than the first-round best binder and > or = 12 500-fold better than LACI-D1. EPI-P302 also shows very high specificity for PLA vs other human proteases and is resistant to inactivation by oxidants and extremes of temperature or pH. Thus, one can use selectants from one library to design target-tailored combinatorial libraries and obtain quite stable, highly specific, very high-affinity binding molecules while maintaining an essentially human framework.     

On burst rescheduling in OBS networks with partial wavelength conversion capability

The core nodes in an optical burst switching (OBS) network are normally equipped with wavelength converters (WCs) to reduce the burst loss probability. Since WCs are expensive and still immature technologically, it is desirable to reduce the number of WCs in the network and to have partial wavelength conversion capability at the core nodes. Nevertheless, a majority of algorithms in the literature are proposed under the full wavelength conversion assumption. As a result, they do not consider the burst loss caused by insufficient WCs, i.e., bursts dropped due to the unavailability of free WCs to convert them to unused wavelengths. In this paper, we demonstrate how to use burst rescheduling to decrease the burst loss due to insufficient WCs and hence cut down on the overall burst loss probability in OBS networks. Two burst rescheduling algorithms are proposed. Their effectiveness in reducing the overall burst loss probability is verified through simulation experiments.     

Accurate online video tagging via probabilistic hybrid modeling

Accurate video tagging has been becoming increasingly crucial for online video management and search. This article documents a novel framework called comprehensive video tagger (CVTagger) to facilitate accurate tag-based video annotation. The system applies both multimodal and temporal properties combined with a novel classification framework with hierarchical structure based on multilayer concept model and regression analysis. The advanced architecture enables effective incorporation of both video concept dependency and temporal dynamics. Using a large-scale test collection containing 50,000 YouTube videos, a set of empirical studies have been carried out and experimental results demonstrate various advantages of CVTagger over the state-of-the-art techniques.     

The synthesis of elastin, collagen, and glycosaminoglycans by high density primary cultures of neonatal rat aortic smooth muscle. An ultrastructural and biochemical study

Primary cultures of neonatal rat aortic smooth muscle cells inoculated at high densities (1 X 10(6) cells/25 cm2 Falcon flask) with adequate nutrient media and pH control grow rapidly and form multilayers of cells with typical "hill and valley" organization. After 10 days growth insoluble elastin formation could be visualized by phase contrast microscopy as small particles which grew rapidly to become larger irregular refractile aggregates and later coalesced to form larger aggregates and small fibres. With light and electronmicroscopy, elastin was the predominant matrix protein formed, with the "hill regions" of cultures containing abundant elastin aggregates and some collagen. In 2-week-old cultures differentiation could be observed within the cell multilayer. The older deeper cells contained more protein synthesis organelles and myofilaments and were in close association with large often coalescing elastin aggregates; compared to younger more superficial cells which contained more free polyribosomes less myofilaments, and were associated with fewer and small elastin aggregates. In older cultures this differentiation was not apparent; the cells contained many myofilaments, dense bodies, and lysosomes. Elastin aggregates and newly formed elastic fibres were abundant in the matrix. Quantitative analysis of insoluble elastin formation in the cell layer during the 4-week culture period indicated continuous biosynthesis and deposition which paralleled that of desmosine formation. Amino-acid analysis of a hot alkali insoluble residue (regarded as elastin) from 30-day-old cultures gave a profile identical with neonatal rat aortic elastin in vivo. Insoluble collagen formation in the cell layer tended to plateau after the log phase of growth was completed (10 days). Proteoglycans were found predominantly in the supernatant media. Glycosaminoglycan analysis revealed a profile of dermatan sulphate (32%), chondroitin 4-sulphate (43%), keratan and heparan sulphate (30%), with only a trace of hyaluronic acid. This study indicates that primary cultures of neonatal rat aortic smooth muscle cells remain differentiated in culture and have the unique capacity to continue to synthesize and deposit large amounts (mg) of insoluble elastin which aggregate and from elastic fibres in vitro.     

1.55μm SiGe 2×3光开关

利用多模干涉效应和自由载流子等离子体色散效应设计和模拟了基于1.55μm波长的2×3 SiGe光开关.该光开关由两个单模输入端口、一个多模干涉区和三个单模输出端口构成.在多模干涉区,设有两个折射率调制区,可以利用来把从两个输入端口输入的光信号分别从三个输出端口输出.束传播法分析结果表明,该光开关的传输损耗小于1.43dB,串扰在-18～-32.8dB之间.     

Key Constraints Analysis with Integrated Production Scheduler

Project delays due to late availabilities of resource and information (RI) prerequisites are one of the major threats to construction management. It is desirable to avoid such delays through better means of constraint management. With most contemporary planning methodologies and tools, it is generally difficult to represent many hidden flow constraints in a construction work plan. However, evaluating the impact of flow constraints is crucial in determining their criticalities, based on which the flow constraints may be prioritized and consequently resolved to minimize project delays. Due to the fact that limited resources are often shared among various trades, it may not be practical to resolve all the constraints simultaneously so that a tradeoff is inevitable, which suggests that management should focus on the most important ones termed as key constraints (i.e., those directly contributing to project delays). This paper presents a methodology that augments the traditional critical path method with RI availability constraints to analyze the causes of delays and locate the key constraints binding on project completion without ambiguity based on the principles of the theory of constraints. The methodology of key constraint analysis has been implemented with the integrated production scheduler, a constraint-based scheduling tool which facilitates the modeling, analysis, and management of constraints at the production planning level. An illustrative example is depicted to demonstrate how the proposed methodology works.