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91.
Peter Stone 《Information & Communications Technology Law》2013,22(1):14-26
In a series of rulings given in the last few years, the Court of Justice of the European Union (EU) has made use of the concept of targeting by a trader of a particular territorial market as a device for determining the scope of European legislation on private law. The concept is of particular importance in the context of trading activities conducted by means of the Internet, though its significance is not confined to such activities. In particular, the European Court has used the concept of targeting for the purpose of restricting the substantive ambit of intellectual property rights created or harmonised by EU legislation. On the other hand, it has avoided use of the concept of targeting in the context of EU legislation allocating jurisdiction between the courts of the Member States, even in respect of disputes involving intellectual property, except where a legislative text specifically invokes such a concept. The instant paper endeavours to review the position as currently established by the European case-law, and to predict the likely further developments. 相似文献
92.
Stone NJ 《Human factors》2008,50(3):534-539
OBJECTIVE: The major contributions of human factors to education are highlighted. BACKGROUND: Over the past 50 years, the education of human factors specialists has evolved, as well as the application of human factors and ergonomic knowledge to education. METHOD: Human factors and ergonomics professional documentation and literature were reviewed to identify major events relevant to human factors education or the application of human factors to education. RESULTS: Human factors education has evolved from training in experimental psychology to highly specialized accredited human factors programs and a number of undergraduate programs, leading to program accreditation and the certification of professionals. In addition, human factors specialists have applied their knowledge to human factors education and, more recently, to educational systems in general. The greatest focus has been on technology such as multimedia. Others have evaluated the design of the physical environment, focusing primarily on seating. The research also often targets undergraduate or graduate education. Therefore, it has been proposed that a greater focus is needed at the K-12 educational level, especially given the advancement and implementation of technology in the classroom. CONCLUSION: Human factors and ergonomic expertise can benefit the educational system. Yet, there is a need to constantly evaluate the benefits of new technology in the classroom as well as the environmental design aspects of the educational environment while considering learners of different age groups, ethnicities, and sexes. APPLICATION: Better application of human factors and ergonomics to the learning environment could enhance the educational experience for all learners. 相似文献
93.
A new process for stabilizing the frequency of commercially available 543-nm He-Ne lasers is described. The stabilization method is based on anomalous dispersion of the gain medium. A total of four green lasers have been stabilized-two at the National Institute of Standards and Technology and two at the Institute of Scientific Instruments of the Czech Academy of Sciences-making it possible to study frequency variations of the lasers relative to each other. We have also stabilized a 633-nm laser by the use of the same method used for 543 nm. 相似文献
94.
S Hicks G Frankel JB Kaper G Dougan AD Phillips 《Canadian Metallurgical Quarterly》1998,66(4):1570-1578
Attaching and effacing (A/E) lesion formation is central to enteropathogenic Escherichia coli (EPEC) pathogenesis. In vitro experiments with human epithelial cell lines have implicated virulence plasmid-encoded bundle-forming pili (BFP) in initial binding and intimin in intimate attachment and A/E lesion formation. This study investigated the role of BFP and intimin in EPEC interactions with pediatric small intestinal biopsy tissue in in vitro organ culture. Organ culture infections (2 to 8 h) were performed with E2348/69 (a wild-type EPEC O127:H6 clinical isolate) and E2348/69 derivatives including CVD206 (eae deficient), CVD206(pCVD438) (eae-complemented CVD206), CVD206(pCVD438/01) (expressing intimin, which is nonfunctional due to a single amino acid substitution), JPN15 (spontaneous EPEC adherence factor virulence plasmid-cured E2348/69), and 31-6-1(1) (E2348/69 with a TnphoA insertion inactivation mutation in the virulence plasmid-encoded bfpA gene). Scanning and transmission electron microscopy revealed that after 8 h E2348/69 and CVD206 (pCVD438) (both Int+ BFP+) adhered to all specimens, causing A/E lesions with surrounding microvillous elongation. JPN15 and 31-6-1(1) (both Int+ BFP-) adhered and caused A/E lesions although bacteria adhered in "flat," two-dimensional groups. CVD206 and CVD206(pCVD438/01) (both Int- BFP+) did not adhere to any sample, and no pathological tissue changes were seen. Thus, in human intestinal organ culture, BFP do not appear to be involved in the initial stages of EPEC nonintimate adhesion but are implicated in the formation of complex, three-dimensional colonies via bacterium-bacterium interactions. Intimin appears to play an essential role in establishing colonization of EPEC on pediatric small intestinal tissue. 相似文献
95.
BJ van Vlijmen NJ Pearce M Berg? B Staels JW Yates AD Gribble BC Bond MH Hofker LM Havekes PH Groot 《Canadian Metallurgical Quarterly》1998,48(4):396-402
PURPOSE: We report an investigation into the distribution of proteoglycans (PGs) in normal, organ-cultured and dextran-treated human corneas. METHODS: Immunogold labeling was carried out at the electron microscope level to localize keratan sulphate (KS), chondroitin sulphate (CS), and heparan sulphate (HS) PGs. RESULTS: High levels of labeling for CS was found in the epithelium, endothelium, and keratocytes, with light labelling present in the basement membranes and the corneal stroma. Labeling for HS was present in the epithelium, endothelium, and keratocytes, with intense labeling present at the endothelium/Descemet's membrane interface and the epithelium/Bowman's layer interface. Large filaments were also observed in these regions in cuprolinic blue-stained specimens. Keratan sulphate was present at high levels in the stroma and the basement membranes with low levels present within the keratocytes, epithelium, and endothelium. The pattern of KS labeling along the collagen fibrils in the stroma sometimes showed evidence of periodicity. Organ-cultured corneas had extensive collagen-free "lakes," the interior of which immunolabeled positively for KS and showed staining with cuprolinic blue. The lakes were greatly reduced in the dextran-treated samples. CONCLUSION: This investigation determined the ultrastructural distribution of KS, CS, and HS PGs in human cornea and showed that organ culture is associated with a change in distribution of stromal PGs. 相似文献
96.
RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs 总被引:1,自引:0,他引:1
JO Ebinu DA Bottorff EY Chan SL Stang RJ Dunn JC Stone 《Canadian Metallurgical Quarterly》1998,280(5366):1082-1086
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of "EF hands" that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling and changes in cell morphology. Signaling was associated with partitioning of RasGRP protein into the membrane fraction. Sustained ligand-induced signaling and membrane partitioning were absent when the DAG-binding domain was deleted. RasGRP is expressed in the nervous system, where it may couple changes in DAG and possibly calcium concentrations to Ras activation. 相似文献
97.
The intensity of high-order rainbows for normally incident light and certain rotation angles of a cylinder with an elliptical cross section is greatly amplified with respect to the intensity for a circular cross-sectional cylinder. The amplification is due to a number of the internal reflections occurring past the critical angle for total internal reflection, and the effect is especially strong for odd-order rainbows, beginning with the third order. Experimentally, the fourth- and the fifth-order rainbows of a nearly elliptical cross-sectional glass rod were observed and analyzed. 相似文献
98.
Targ R Steakley BC Hawley JG Ames LL Forney P Swanson D Stone R Otto RG Zarifis V Brockman P Calloway RS Klein SH Robinson PA 《Applied optics》1996,35(36):7117-7127
The use of airborne laser radar (lidar) to measure wind velocities and to detect turbulence in front of an aircraft in real time can significantly increase fuel efficiency, flight safety, and terminal area capacity. We describe the flight-test results for two coherent lidar airborne shear sensor (CLASS) systems and discuss their agreement with our theoretical simulations. The 10.6-μm CO(2) system (CLASS-10) is a flying brassboard; the 2.02-μm Tm:YAG solid-state system (CLASS-2) is configured in a rugged, light-weight, high-performance package. Both lidars have shown a wind measurement accuracy of better than 1 m/s. 相似文献
99.
100.