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101.
W Ramos N Tappe J Talamantez EC Friedberg AE Tomkinson 《Canadian Metallurgical Quarterly》1997,25(8):1485-1492
Four biochemically distinct DNA ligases have been identified in mammalian cells. One of these enzymes, DNA ligase I, is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae CDC9 gene. Cdc9 DNA ligase has been assumed to be the only species of DNA ligase in this organism. In the present study we have identified a second DNA ligase activity in mitotic extracts of S. cerevisiae with chromatographic properties different from Cdc9 DNA ligase, which is the major DNA joining activity. This minor DNA joining activity, which contributes 5-10% of the total cellular DNA joining activity, forms a 90 kDa enzyme-adenylate intermediate which, unlike the Cdc9 enzyme-adenylate intermediate, reacts with an oligo (pdT)/poly (rA) substrate. The levels of the minor DNA joining activity are not altered by mutation or by overexpression of the CDC9 gene. Furthermore, the 90 kDa polypeptide is not recognized by a Cdc9 antiserum. Since this minor species does not appear to be a modified form of Cdc9 DNA ligase, it has been designated as S. cerevisiae DNA ligase II. Based on the similarities in polynucleotide substrate specificity, this enzyme may be the functional homolog of mammalian DNA ligase III or IV. 相似文献
102.
We have examined the applicability of this simple and effective test to polymer systems widely used in the medical industry. First, it was found that not every polymer examined exhibited a distinct onset of autocatalytic oxidation detectable by DSC. However, for cases where distinct onsets are found, powerful insights can be obtained. These included activation energy of the degradation, thermal oxidative shelf life prediction, and correlation, with product performances. Actual examples are presented to illustrate the utility of the test. 相似文献
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KI Schwendner AE Mikesky WS Holt M Peacock DB Burr 《Canadian Metallurgical Quarterly》1997,52(3):M155-M160
BACKGROUND: Significant morbidity and mortality are associated with falls in older adults. We tested the hypothesis that older women with a history of falls demonstrate decreased muscle endurance and longer recovery times following fatiguing exercise. METHODS: We evaluated dynamic endurance and recoverability of the quadriceps femoris of 29 young women (YW) (M age = 21.7), 26 older women with a history of falls (FA) (M age = 73.3), and 27 older women with no history of falls (NF) (M age = 71.2) using an isokinetic dynamometer. Subjects performed repeated maximal concentric knee extensions until the force output of two consecutive repetitions fell below 50% of their maximal voluntary contraction (MVC). Recovery was defined as the time required for the return of force output > or = 80% MVC for 2 consecutive repetitions, within a set consisting of 3 maximal contractions. One minute rest was allowed between sets. We collected electromyographic (EMG) data from the quadriceps during all testing to evaluate spectral shifts. RESULTS: ANOVA with a post-hoc Bonferroni-Dunn test revealed time to fatigue was significantly faster in FA than YW (p < .02) and in FA than NF (p < .05), but not different between YW and NF. Time to recovery was significantly slower in FA than YW (p = .01), but not different between YW and NF, or between FA and NF, EMG median frequency power shift (from the beginning to the end of the test) was significantly less in FA (p < .001) than either YW (p < .002) or NF (p < .05). CONCLUSIONS: Older women with a history of falls demonstrate decreased muscular endurance compared to YW and NF, and increased time to recover from fatiguing exercise when compared to young women. 相似文献
106.
EFFECTS OF COLD EXPANSION OF A HOLE ON FATIGUE CRACK INITIATION LOCATION AND LIFE OF AN LY12CZ ALLOY
Ling Chao Zheng Xiulin† 《Fatigue & Fracture of Engineering Materials & Structures》1992,15(3):241-247
Abstract— In the present study the effects of cold expansion of a hole on the location of fatigue crack initiation and life of LY12CZ alloy sheets are experimentally investigated and a quantitative expression of fatigue crack initiation life presented. Test results and analysis show that there may exist an optimum amount of cold expansion, for which both the coefficient of the resistance to fatigue crack initiation and the threshold are increased, and that the direction of cold expansion has no appreciable effect on fatigue crack initiation life. Observations show that, after cold expansion the fatigue crack nearly always initiates on the final entry face from which the mandrel is introduced into the hole during the final cold expansion process. Therefore it may be thought that the entry face is less resistant to fatigue crack initiation. In addition, the assumption of equivalence of residual stresses and also the strain hardening conditions on both the entry and exit faces may be questionable. 相似文献
107.
Ling Tong 《Journal of Infrared, Millimeter and Terahertz Waves》1997,18(2):431-436
This paper introduce the experiment about a new type of quasi-optic cavity Making a experimental model, measuring all parameters of the cavity, giving experiment results. It shows that theoritical research in reference[1] is correct Its results coincide with theory. At same time, it also indicates the cavity is hopeful device in MMW. 相似文献
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泡沫铝的动态压缩性能和吸能性研究 总被引:9,自引:0,他引:9
通过测量泡沫铝在动态和准静态压缩条件下的应力 -应变曲线 ,研究了泡沫铝的准静态和动态压缩行为以及不同应变条件下的吸能性 ,并对其应变率效应进行了分析。结果表明 ,在高应变速率和准静态压缩下 ,泡沫铝的σ -ε曲线均表现出弹性变形段、平缓段和密实段三阶段特征 ;泡沫铝的压缩性能具有明显的应变速率敏感性 ,随应变速率的提高 ,流动应力上升 ,吸能性升高 相似文献