全文获取类型
收费全文 | 2693篇 |
免费 | 33篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 8篇 |
综合类 | 1篇 |
化学工业 | 210篇 |
金属工艺 | 15篇 |
机械仪表 | 57篇 |
建筑科学 | 24篇 |
矿业工程 | 3篇 |
能源动力 | 9篇 |
轻工业 | 143篇 |
水利工程 | 7篇 |
石油天然气 | 5篇 |
无线电 | 31篇 |
一般工业技术 | 87篇 |
冶金工业 | 2060篇 |
原子能技术 | 2篇 |
自动化技术 | 65篇 |
出版年
2024年 | 2篇 |
2023年 | 10篇 |
2022年 | 30篇 |
2021年 | 49篇 |
2020年 | 26篇 |
2019年 | 30篇 |
2018年 | 24篇 |
2017年 | 24篇 |
2016年 | 19篇 |
2015年 | 13篇 |
2014年 | 25篇 |
2013年 | 56篇 |
2012年 | 50篇 |
2011年 | 33篇 |
2010年 | 26篇 |
2009年 | 26篇 |
2008年 | 32篇 |
2007年 | 24篇 |
2006年 | 30篇 |
2005年 | 20篇 |
2004年 | 11篇 |
2003年 | 14篇 |
2002年 | 10篇 |
2001年 | 10篇 |
2000年 | 20篇 |
1999年 | 84篇 |
1998年 | 623篇 |
1997年 | 364篇 |
1996年 | 226篇 |
1995年 | 143篇 |
1994年 | 104篇 |
1993年 | 122篇 |
1992年 | 13篇 |
1991年 | 20篇 |
1990年 | 16篇 |
1989年 | 20篇 |
1988年 | 24篇 |
1987年 | 14篇 |
1986年 | 16篇 |
1985年 | 17篇 |
1983年 | 8篇 |
1982年 | 8篇 |
1981年 | 20篇 |
1980年 | 28篇 |
1978年 | 6篇 |
1977年 | 66篇 |
1976年 | 160篇 |
1975年 | 3篇 |
1964年 | 1篇 |
1955年 | 3篇 |
排序方式: 共有2727条查询结果,搜索用时 15 毫秒
101.
JM César AG Avello A Vecino C Cerveró JG Lara?a IF Fuertes J Villarrubia J López JP de Oteyza JL Velasco A Cantalapiedra P Herrera S Herrero JL Navarro 《Canadian Metallurgical Quarterly》1998,111(16):601-603
BACKGROUND: To describe the main characteristics and response to desmopressin infusion in 103 patients suffering from von Willebrand disease (vWD). PATIENTS AND METHODS: The criteria for diagnosis were (except for type 2N) the coexistence of von Willebrand factor ristocetin cofactor (vWF:RCo) activity < 50 U/dl with bleeding disease or one of the following data: von Willebrand factor antigen (vWF:Ag) activity < 50 U/dl, factor VIII (FVIII) activity < 50 U/dl or the existence of a increased bleeding time (BT). Multimeric studies of vWF were performed in 51 cases and ristocetin induced platelet aggregation (RIPA) was also performed. RESULTS: Spontaneous bleeding was found in 36 patients, while in 18 cases the diagnosis was done after surgical bleeding. Thirteen patients (6 presenting with mild bleeding) were studied for abnormalities in the routine preanestesic tests. Other 22 patients were diagnosed with vWD by familial studies. There were 3 patients with type 2B, 1 case with type 2N and other patient with type 3. BT was found increased in 26 out of 58 patients. The activities of vWF:CoR and vWF:Ag were 38.4 (9.4) U/dl and 45.8 (23.2) U/dl, respectively, while the activity of FVIII was 49.9 (20.8) U/dl. Prophylactic DDAVP (desmopressin) was infused in 32 patients. After 1 h, basal activities of vWF:CoR and vWF:Ag were increased by 3.1 (3.2) and 3.4 (3.1) times, respectively, and maintained for 3 h. FVIII activity increased 3.6 (2.3) times the basal levels decreasing after 3 h (2.9 [2.1]; p < 0.01). The BT was corrected in 8 out of ten patients. CONCLUSIONS: vWD is a major cause of surgical bleeding. Preanestesic anamnesis and coagulation tests can be useful to identify vWD. Many patients with vWD have normal BT. A failure in the response to desmopressin infusion is unusual. 相似文献
102.
The recent rapid growth of protein sequence databases is outpacing the capacity of researchers to biochemically and structurally characterize new proteins. Accordingly, new methods for recognition of motifs and homologies in protein primary sequences may be useful in determining how these proteins might function. We have applied such a method, an iterative learning algorithm, to analyze possible coiled coil domains in histidine kinase receptors. The potential coiled coils have not yet been structurally characterized in any histidine kinase, and they appear outside previously noted kinase homology regions. The learning algorithm uses a combination of established sequence patterns in known coiled coil proteins and histidine kinase sequence data to learn to recognize efficiently this coiled coil-like motif in the histidine kinases. The common appearance of the structural motif in a functionally important part of the receptors suggests hypotheses for kinase regulation and signal transduction. 相似文献
103.
104.
Expression of the third component of complement, C3, in regenerating limb blastema cells of urodeles 总被引:1,自引:0,他引:1
K Del Rio-Tsonis PA Tsonis IK Zarkadis AG Tsagas JD Lambris 《Canadian Metallurgical Quarterly》1998,161(12):6819-6824
In this study we have shown that complement component C3 is expressed in the regenerating tissue during urodele limb regeneration. C3 was expressed in the dedifferentiated regeneration blastema and in the redifferentiated limb tissues in the axolotl, Amblystoma mexicanum, and in Notophthalmus viridescens. This expression was verified by immunofluorescent staining using an Ab against axolotl C3 and by in situ hybridization with an axolotl C3 cDNA probe. In the early stages of regeneration C3 appeared to be equally present in all mesenchymal cells and in the wound epithelium, whereas in the later stages it was mainly expressed in the differentiating muscle cells. Since no expression was seen in the developing limb, it appears that the C3 expression was specific to the regeneration process. We then demonstrated by hybridization experiments that a blastema cell line of myogenic origin expresses C3. All these findings implicate C3 in the dedifferentiation process and may indicate a new role for this molecule in muscle differentiation. 相似文献
105.
BACKGROUND AND OBJECTIVE: Intensive induction and post-remission therapies have improved the prognosis in adult acute lymphoblastic leukemia (ALL). However, different from children, the impact of late intensification therapy in the overall results of treatment has not been consistently evaluated. The objective of this study was to analyze the results of a multicenter prospective protocol, PETHEMA ALL-89, in which, after intensive induction and consolidation therapy, randomization to receive delayed intensification treatment was performed. DESIGN AND METHODS: One hundred and eight adults (age > or = 15 years) diagnosed with ALL (ALL L3 excluded) in 22 Spanish hospitals from 1989 to 1994 were treated with a five-drug induction therapy, followed by four cycles of early post-remission treatment during four months, and maintenance therapy for two years. Patients in remission at the end of the first year were randomized to receive one six-week cycle of late intensification therapy. Uni- and multivariate analyses of early response to treatment, complete remission (CR), leukemia-free survival (LFS) and overall survival (OS) were performed. RESULTS: The median (range) age of the series was 28 (15-74) years and leukocyte count 26 x 10(9)/L (1-600). ALL L1/L2 was present in 38/70 patients, early pre-B in 13, common in 53, pre-B in 12 and T in 30 cases. The CR rate was 86%, and refractory disease 9%. Median LFS was 34 months, with a 5-yr probability of 41% (95% CI, 29-53), whereas median OS was 51 months and 5-year probability 47% (34-59%). There were no differences in either LFS and OS between patients who did or did not receive delayed intensification therapy. Prognostic factors for CR attainment were advanced age and slow response to therapy. These two features were, in addition to high leukocyte counts, the parameters with negative influence in both LFS and OS. INTERPRETATION AND CONCLUSIONS: The results of PETHEMA ALL-89 are similar to those referred in other chemotherapy-based protocols in adult ALL. Delayed intensification has not improved the length of remission and survival. Efforts to improve the prognosis of adult ALL patients must be mainly focused in early intensification treatment. 相似文献
106.
B Arvin D Lekieffre JL Graham C Moncada AG Chapman BS Meldrum 《Canadian Metallurgical Quarterly》1994,62(4):1458-1467
The effect of the non-N-methyl-D-aspartate (non-NMDA) receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8-methylenedioxy-5H-2,3-benzodiazepine hydrochloride (GYKI 52466) on ischaemia-induced changes in the microdialysate and tissue concentrations of glutamate, aspartate, and gamma-aminobutyric acid (GABA) was studied in rats. Twenty minutes of four-vessel occlusion resulted in a transient increase in microdialysate levels of glutamate, aspartate, and GABA in striatum, cortex, and hippocampus. Administration of GYKI 52466 (10 mg/kg bolus + 10 mg/kg/60 min intravenously starting 20 min before onset of ischaemia) inhibited ischaemia-induced increases in microdialysate glutamate and GABA in striatum without affecting the increases in hippocampus or cortex. Twenty minutes of four-vessel occlusion resulted in immediate small decreases and larger delayed (72 h) decreases in tissue levels of glutamate and aspartate. Transient increases in tissue levels of GABA were shown in all three structures at the end of the ischaemic period. At 72 h, after the ischaemic period, significantly reduced GABA levels were observed in striatum and hippocampus. GYKI 52466, given under identical conditions as above, augmented the ischaemia-induced decrease in striatal tissue levels of glutamate and aspartate, without significantly affecting the decreases in hippocampus and cortex. Twenty minutes of ischaemia resulted in a large increase in microdialysate dopamine in striatum. GYKI 52466 failed to inhibit this increase. Kainic acid (500 microM infused through the probe for 20 min) caused increases in microdialysate glutamate and aspartate in the striatum. GYKI 52466 (10 mg/kg bolus + 10 mg/kg/60 min) completely inhibited the kainic acid-induced glutamate release. In conclusion, the action of the non-NMDA antagonist, GYKI 52466, in the striatum is different from that in the cortex and hippocampus. The inhibition by GYKI 52466 of ischaemia-induced and kainate-induced increases in microdialysate glutamate concentration in the striatum may be related to the neuroprotection provided by GYKI 52466 in this region. 相似文献
107.
108.
KL Hammond IM Hanson AG Brown LA Lettice RE Hill 《Canadian Metallurgical Quarterly》1998,74(1-2):121-131
The human myeloid leukemias are a diverse group of disorders characterized by massive clonal expansion of myeloid cells showing variable degrees of differentiation block. Leukemic dendritic cells were generated in culture from chronic myelogenous leukemia (CML). These were used to stimulate autologous T cells to develop leukemia-specific cytotoxicity. Available data suggest that the cells responsible for the cytolytic activity are at least in part CD8+ and HLA restricted in their function. Additional data suggest that some anti-CML cellular activity may be Fas mediated. T-cell receptor studies provide evidence for an oligoclonal response implying a recognition of a limited number of antigens. We have used culture techniques similar to those used for CML to study the ability of AML cells to differentiate toward dendritic cells. Four of five patients have shown acute leukemia-derived dendritic cells. This work offers an avenue for the development of novel strategies for the control of human myeloid leukemias. 相似文献
109.
E Sepet Z Aytepe AG Ozerkan N Yalman Y Guven S Anak O Devecioglu L Agaoglu G Gedikoglu 《Canadian Metallurgical Quarterly》1998,22(3):257-260
Five and seven membered constrained alpha-amino acid derivatives were synthesized using ring-closing metathesis reaction as a key step. 相似文献
110.