Reduction of disulfide bonds in an amyloidogenic Bence Jones protein leads to formation of "amyloid-like" fibrils in vitro

Motivated by the finding that the amino acid sequence of the Bence Jones protein BJP-DIA was identical to that of the main protein component of the amyloid fibrils obtained from the same patient with AL-amyloidosis, (Klafki, H.-W., Kratzin, H.-D., Pick, A.-I., Eckart, K., Karas, M. & Hilschmann, N. (1992) Biochemistry 31, 3265-3272.), we attempted to create "amyloid-like" fibrils from the Bence Jones protein in vitro, without addition of proteolytic enzymes. Reduction of BJP-DIA, solubilized in PBS, pH 7.4, overnight at 37 degrees C resulted in the formation of a precipitate which had affinity for the dye Congo red. Electron microscopy of negatively stained samples of the reduced protein revealed aggregates of linear unbranched fibrils. SDS-polyacrylamide gel electrophoresis demonstrated that the precipitate consisted almost exclusively of intact light chain molecules. This result makes it possible to deduce a molecular model of these amyloid fibrils generated in vitro.     

代理盲签名的不可跟踪性分析

张学军在《基于身份的代理盲签名方案的分析与改进》(《计算机工程》,2009年第23期)一文中分析指出,农强等的代理盲签名方案(《计算机应用》,2008年第8期)不具有不可跟踪性。对此,通过反证法证明张学军的方法有误,在此基础上提出一种新的分析方法,利用其证明胡江红的方案(《计算机工程与应用》,2007年第18期)不满足不可跟踪性,并对该方案进行改进,使其满足不可跟踪性且效率得到提高。     

Ultrastructural and biochemical changes of hepatocytes during ether and fluothane anesthesia under experimental conditions

A study was made of a possibility of using Soviet made membranes "Vladipor" with a pore diameter of 16--17 nm for the purpose of concentration and purification of antigenic extracts from stab cultures of S. typhi obtained by the action of typsin, hydrogen peroxide and detergents. Preparations, complete by antigenic structure, were obtained by ultrafiltration, with a good yield. The maximal rate of ultrafiltration was noted in case of "peroxide" antigenic preparations. The use of diafiltration provided a significant reduction of the ash content in the end products.     

The critical control of progesterone levels and pregnancy by antiprogesterone

Circulating plasma progesterone (P) has been quantitatively controlled in the rat "model" through highly specific binding by treatment with anti-P (A-P). Knowing the constant, which characterizes the binding of P to A-P in plasma, sequential assays of circulating A-P and A-P bound total P (Pt) revealed the levels of the biologically active unbound P (Pu). The studies showed that at different stages of gestation the mechanisms through which A-P reduces Pu and terminates pregnancy are the same. However, the doses of AP which effectively reduce Pu and also the critical levels of Pu at which pregnancy terminates are different. The moderate and transient physiologic P-withdrawal (Pw) at midterm permits the continuation of normal gestation, but pregnancy is terminated by a drastic and sustained reduction in Pu. In contrast, when Pu is only slightly and briefly reduced below physiologic levels, pregnancy continues and only retarded conceptus growth signals that Pw occurred. Apparently Pw has to be controlled and measured with "razor's-edge" precision to fully expose and define the regulatory significance of this steroid in the maintenance and termination of pregnancy. Short of this precision, the key regulator of the pregnant uterus will remain buried, as it has been during 40 years, in controversial findings.     

Morphology of pneumopathies and their role in the development of asphyxia neonatorum

Histories of deliveries and development records of 44 newborns with penumopathies who had died on the first days of life in the presence of phenomena of the syndrome of respiratory disorder were analysed and the newborns' lungs were morphologically investigated. The investigation made it possible to single out the following forms of pneumopathy in newborns: edematous-hemorrhagic, hyaline, atelectatic, aspirated, and mixed. In the majority of cases the syndrome of respiratory disorder was a sequela of hypoxia of the intrauterine origin which continued during the first hours and days of life after birth. Such "primary asphyxia" may lead to the development of pneumopathy in a neonate. On the other hand, "secondary asphyxia" in many cases represent sequelae of penumopathy destructural and functional immaturity of the pulmonary tissue, hypoxia, a higher permeability of vessels of the microcirculatory bed, spasm of small pulmonary arteris contributed considerably to the development of penumopathies.     

飞机训练模拟器视景系统非线性失真校正技术研究

柱面实像投影是飞机训练模拟器视景系统采用的主要投影方式之一,可以得到很大的观察视野,但是不可避免地会产生非线性失真。根据具体的工程背景,针对大视场角的柱面实像投影的非线性失真,分析了其产生的原因,并设计了投影仪的离轴安装方案,从而提出了基于预失真的柱面投影非线性失真校正算法。实际的校正结果表明,采用预失真的方法进行柱面非线性失真校正,是一种行之有效的方法。通过相应的数据结构设计,保证了程序执行的实时性。     

H.264编码工具性能及实时性研究

H．264具有更好的编码效率，但其高复杂度无法满足实时应用。针对低码率应用，分析各编码工具的性能并选取合理编码框架，同时利用单指令多数据（SIMD）技术并行实现快速搜索及整数变换。     

高炉紫铜风口的焊接

高炉紫铜风口是由铸造本体和轧制端头两个部分采用TIG手工焊接而成。随着高炉的扩容，为了延长风口使用寿命，提高焊缝物理和力学性能，成功采用了MIG自动焊，满足了高炉生产需求。     

650轧辊的堆焊修复

对轧辊断裂部位进行了分析，找出了裂纹形成的主要原因。通过采取焊前预热、低氢焊接和焊后热处理等措施，成功地实现了650轧辊端头断裂的堆焊修复，即以焊代换。同时,延长了轧辊服役周期。     

全串行单片机系统在光纤气敏传感器中的应用

针对传统电路硬件复杂及可靠性差等问题，介绍了利用AT89C51单片机和相应的SPI串行接口芯片，构成的一种光纤气敏传感器的数据采集和数据处理部分，在分析了各串行芯片的特点后，详细介绍了该单片机系统的硬件接口电路和软件程序，由于该光纤气敏传感器具有电路简单，体积小，运行可靠等特点，因而具有很好的应用前景。