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Validating smoking data from the Veteran's Affairs Health Factors dataset, an electronic data source
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Nadeem Ullah Ghazala Yasmeen Butt Mehwish Jaffer Quratul Ain 《Microscopy research and technique》2022,85(2):748-754
The study was carried out in four districts, that is, Gujranwala, Gujarat, Narowal and Sialkot of Punjab, Pakistan. The sampling was carried out randomly in different seasons from the water bodies especially from wastewater. Twenty-one species belonging to Euglenophycota were identified using light microscopy and scanning electron microscopy from which 04 species belong to genus Phacus, 02 species belonging to Trachelomonas and Euglena based on light microscopy. It was observed that Euglena was the most diverse genus and it is supposed to be the indicator species of the polluted water. It was observed that E. oblonga was found in maximum pH range, that is, 7.0–11.0. Similarly, E. brevicaudatus was found in maximum EC, that is, 169 ± 1.5 ms/cm these outcomes indicated that for internal examination along with LM, SEM was necessary for correct identification of algal sample up to specie level. 相似文献
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Basak AK; Kroone RC; Lubsen NH; Naylor CE; Jaenicke R; Slingsby C 《Protein engineering, design & selection : PEDS》1998,11(5):337-344
The 2-domain gammaS-crystallin, a highly conserved early evolutionary
off-shoot of the gamma-crystallin family, is located in the water-rich
region of eye lenses. The expressed C-terminal domain, gammaS-C, has been
crystallized and the 2.56 A X-ray structure determined. There are two
domains in the asymmetric unit which pair about a distorted twofold axis.
One of the domains has an altered conformation in a highly conserved region
of the protein, the tyrosine corner. The distorted gammaS-C dimer of
domains is compared with the highly symmetrical, equivalent recombinant
dimer of C-terminal domains from gammaB- crystallin. Sequence changes close
to the interface, that distinguish gammaS from the other gamma-crystallins,
are examined in order to evaluate their role in symmetrical domain pairing.
相似文献
169.
Muhammad Wahab Amjad Mohd Cairul Iqbal Mohd Amin Haliza Katas Adeel Masood Butt 《Nanoscale research letters》2012,7(1):687
Doxorubicin-loaded micelles were prepared from a copolymer comprising cholic acid (CA) and polyethyleneimine (PEI) for the delivery of antitumor drugs. The CA-PEI copolymer was synthesized via pairing mediated by N,N’-dicyclohexylcarbodiimide and N-hydroxysuccinimide using dichloromethane as a solvent. Fourier transform infrared and nuclear magnetic resonance analyses were performed to verify the formation of an amide linkage between CA and PEI and doxorubicin localization into the copolymer. Dynamic light scattering and transmission electron microscopy studies revealed that the copolymer could self-assemble into micelles with a spherical morphology and an average diameter of <200 nm. The CA-PEI copolymer was also characterized by X-ray diffraction and differential scanning calorimetry. Doxorubicin-loaded micelles were prepared by dialysis method. A drug release study showed reduced drug release with escalating drug content. In a cytotoxicity assay using human colorectal adenocarcinoma (DLD-1) cells, the doxorubicin-loaded CA-PEI micelles exhibited better antitumor activity than that shown by doxorubicin. This is the first study on CA-PEI micelles as doxorubicin carriers, and this study demonstrated that they are promising candidates as carriers for sustained targeted antitumor drug delivery system. 相似文献
170.
The influence of polymer surface-protein binding affinity on protein ion signals in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is examined. The surfaces of poly(vinylidene fluoride) and poly(ethylene terephthalate) polymer substrates are modified by pulsed rf plasma deposition of allylamine. By varying the on/off duty cycle of the pulsed rf plasma, the polymer substrate surfaces are coated with thin films having varying densities of surface amine groups. The varying surface amine density is shown to lead to systematic changes in the surface binding affinity for the 125I-radiolabeled peptides angiotensin I and porcine insulin. Unlabeled angiotensin I and porcine insulin are then deposited on the pulsed rf plasma-modified substrates and analyzed by MALDI mass spectrometry. The experimental approach involves applying the peptide to the modified polymer surface in an aqueous phosphate-buffered saline solution and allowing the peptide solution to dry completely under ambient conditions. Subsequently, the MALDI matrix alpha-cyano-4-hydroxycinnamic acid in methanol and 10% trifluoroacetic acid in water are added to the peptide-coated modified polymer surfaces. The results of these studies demonstrate that, for the sample preparation method employed, increases in the surface peptide binding affinity lead to decreases in the peptide MALDI ion signal. 相似文献