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This paper presents a wavelet transform based tree structure model developed and evaluated for the classification of skin lesion images into melanoma and dysplastic nevus. The tree structure model utilizes a semantic representation of the spatial-frequency information contained in the skin lesion images including textural information. Results show that the presented method is effective in discriminating melanoma from dysplastic nevus. The results are also compared with those obtained using another method of developing tree structures utilizing the maximum channel energy criteria with a fixed energy ratio threshold. 相似文献
135.
Geometric representation and measurements of localized lumen stenosis of coronary arteries are important considerations in the diagnosis of cardiovascular diseases. This discrete narrowing of the arteries typically impairs blood flow in regions of the heart, and can be present along the entire length of the artery. Three-dimensional (3-D) reconstruction of coronary arterial tree allows clinician to visualize vascular geometry. Three-dimensional representation of tree topology facilitates calculation of hemodynamic measurements to study myocardial infarction and stenosis. The 3-D arterial tree, computed from two views, can provide more information about the tree geometry than individual views. In this paper, a 3-step algorithm for 3-D reconstruction of arterial tree using two standard views is presented. The first step is a multi-resolution segmentation of the coronary vessels followed by medial-axis detection along the entire arterial tree for both views. In the second step, arterial trees from the two views are registered using medial-axis representation at the coarsest resolution level to obtain an initial 3-D reconstruction. This initial reconstruction at the coarsest level is then modified using 3-D geometrical a priori information. In the third step, the modified reconstruction is projected on the next higher-resolution segmented medial-axis representation and an updated reconstruction is obtained at the higher resolution. The process is iterated until the final 3-D reconstruction is obtained at the finest resolution level. Linear programming based constrained optimization method is used for registering two views at the coarse resolution. This is followed by a Tree-Search method for registering detailed branches at higher resolutions. The automated 3-D reconstruction method was evaluated on computer-simulated as well as human angiogram data. Results show that the automated 3-D reconstruction method provided good registration of computer-simulated data. On human angiogram data, the computed 3-D reconstruction matched well with manual registration. 相似文献
136.
A. Dhawan V. Zaporojtchenko F. Faupel S. K. Sharma 《Journal of Materials Science》2007,42(21):9037-9044
The oxidation of the bulk amorphous alloy Zr65Cu17.5Ni10Al7.5 in air in its amorphous and the supercooled liquid states was studied in the temperature range 573–663 K using X-ray photoelectron
spectroscopy (XPS). The oxide film mainly consisted of the oxides of Zr (as ZrO2) and Al (as Al2O3). No Cu or Ni was found in the oxide film formed on the amorphous state of the alloy while significant Cu (as CuO) was present
in the oxide film formed on the alloy in its supercooled liquid state. The role of the various alloying elements during oxidation
at high temperatures in air is discussed in the paper. The XPS data from oxide film support the previously suggested mechanism
for oxidation of this alloy, i.e. the rate controlling process during oxidation of the alloy at low temperatures (in the amorphous
state) is the back-diffusion of Ni and Cu, while the oxidation at high temperatures (in the supercooled liquid state) is dominated
by the inward diffusion of oxygen. 相似文献
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This paper describes the design and fabrication of fiber-optic nanoprobes developed for optical detection in single living
cells. It is critical to fabricate probes with well-controlled nanoapertures for optimized spatial resolution and optical
transmission. The detection sensitivity of fiber-optic nanoprobe depends mainly on the extremely small excitation volume that
is determined by the aperture sizes and penetration depths. We investigate the angle dependence of the aperture in shadow
evaporation of the metal coating onto the tip wall. It was found that nanoaperture diameters of approximately 50 nm can be
achieved using a 25° tilt angle. On the other hand, the aperture size is sensitive to the subtle change of the metal evaporation
angle and could be blocked by irregular metal grains. Through focused ion beam (FIB) milling, optical nanoprobes with well-defined
aperture size as small as 200 nm can be obtained. Finally, we illustrate the use of the nanoprobes by detecting a fluorescent
species, benzo[a]pyrene tetrol (BPT), in single living cells. A quantitative estimation of the numbers of BPT molecules detected
using fiber-optic nanoprobes for BPT solutions shows that the limit of detection was approximately 100 molecules. 相似文献
140.
KM Koslowski PR Shaver JT Casey T Wilson G Yamanaka AK Sheaffer DJ Tenney NE Pederson 《Canadian Metallurgical Quarterly》1999,73(2):1704-1707
Herpes simplex virus (HSV) DNA is cleaved from concatemers and packaged into capsids in infected cell nuclei. This process requires seven viral proteins, including UL15 and UL28. UL15 expressed alone displays a nuclear localization, while UL28 remains cytoplasmic. Coexpression with UL15 enables UL28 to enter nuclei, suggesting an interaction between the two proteins. Additionally, UL28 copurified with UL15 from HSV-infected cells after ion-exchange and DNA affinity chromatography, and the complex sedimented as a 1:1 heterodimer upon sucrose gradient centrifugation. These findings are evidence of a physical interaction of UL15 and UL28 and a functional role for UL15 in directing UL28 to the nucleus. 相似文献